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Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens.
Poult Sci. 2020 Jul; 99(7):3628-3636.PS

Abstract

Avian pathogenic Escherichia coli (APEC) is a major pathogen in the poultry industry worldwide including Korea. In this study, the phenotypic and genotypic characteristics of 33 fluoroquinolone (FQ)-resistant APEC isolates from broilers were analyzed. All FQ-resistant APEC isolates showed amino acid exchanges at both gyrA and parC and high minimal inhibitory concentrations for FQs. A total of 11 (33.3%) isolates were positive for the plasmid-mediated quinolone resistance (PMQR) genes, qnrA (8 isolates) and qnrS (3 isolates), and showed multidrug resistance. Among the 11 PMQR-positive isolates, 1 and 2 isolates carried blaCTX-1 and blaCTX-15, respectively, as extended-spectrum β-lactamase (ESBL) producers, and the non-ESBL gene, blaTEM-1, was found in 4 isolates. Among 3 aminoglycoside-resistant isolates, aac(3)-II was only detected in 1 isolate. All 8 APEC isolates with resistance to tetracycline carried the tetA gene. Overall, 6 of the 7 trimethoprim-sulfamethoxazole-resistant isolates carried the sul1 or sul2 genes, while only 2 of the 8 chloramphenicol-resistant isolates carried the catA1 gene. Although 9 isolates carried class I integrons, only 4 isolates carried the gene cassettes dfrA12-aadA2 (2 isolates), dfrA17-aadA5 (1 isolate), extX-psp-aadA2 (1 isolate), and dfrA27 (1 isolate). The most common plasmid replicon was FIB (8 isolates, 72.7%), followed by K/B (4 isolates, 36.4%). Antimicrobial resistance monitoring and molecular analysis of APEC should be performed continuously to surveil the transmission between poultry farms.

Authors+Show Affiliations

Quality Management Department, Samhwa GPS Breeding Agri. Inc., Hongseong-gun, Chung Nam, 32291, Republic of Korea.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Buk-gu, Daegu, 41566, Republic of Korea.Quality Management Department, Samhwa GPS Breeding Agri. Inc., Hongseong-gun, Chung Nam, 32291, Republic of Korea.Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, 39762, USA.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Buk-gu, Daegu, 41566, Republic of Korea.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Buk-gu, Daegu, 41566, Republic of Korea.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Buk-gu, Daegu, 41566, Republic of Korea. Electronic address: youngju@knu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32616259

Citation

Yoon, Mi Young, et al. "Molecular Characteristics of Fluoroquinolone-resistant Avian Pathogenic Escherichia Coli Isolated From Broiler Chickens." Poultry Science, vol. 99, no. 7, 2020, pp. 3628-3636.
Yoon MY, Kim YB, Ha JS, et al. Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens. Poult Sci. 2020;99(7):3628-3636.
Yoon, M. Y., Kim, Y. B., Ha, J. S., Seo, K. W., Noh, E. B., Son, S. H., & Lee, Y. J. (2020). Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens. Poultry Science, 99(7), 3628-3636. https://doi.org/10.1016/j.psj.2020.03.029
Yoon MY, et al. Molecular Characteristics of Fluoroquinolone-resistant Avian Pathogenic Escherichia Coli Isolated From Broiler Chickens. Poult Sci. 2020;99(7):3628-3636. PubMed PMID: 32616259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characteristics of fluoroquinolone-resistant avian pathogenic Escherichia coli isolated from broiler chickens. AU - Yoon,Mi Young, AU - Kim,Yeong Bin, AU - Ha,Jong Su, AU - Seo,Kwang Won, AU - Noh,Eun Bi, AU - Son,Se Hyun, AU - Lee,Young Ju, Y1 - 2020/04/15/ PY - 2019/10/18/received PY - 2020/02/13/revised PY - 2020/03/21/accepted PY - 2020/7/4/entrez KW - APEC KW - antimicrobial-resistant gene KW - broiler KW - multidrug resistance KW - plasmid-mediated quinolone resistance SP - 3628 EP - 3636 JF - Poultry science JO - Poult. Sci. VL - 99 IS - 7 N2 - Avian pathogenic Escherichia coli (APEC) is a major pathogen in the poultry industry worldwide including Korea. In this study, the phenotypic and genotypic characteristics of 33 fluoroquinolone (FQ)-resistant APEC isolates from broilers were analyzed. All FQ-resistant APEC isolates showed amino acid exchanges at both gyrA and parC and high minimal inhibitory concentrations for FQs. A total of 11 (33.3%) isolates were positive for the plasmid-mediated quinolone resistance (PMQR) genes, qnrA (8 isolates) and qnrS (3 isolates), and showed multidrug resistance. Among the 11 PMQR-positive isolates, 1 and 2 isolates carried blaCTX-1 and blaCTX-15, respectively, as extended-spectrum β-lactamase (ESBL) producers, and the non-ESBL gene, blaTEM-1, was found in 4 isolates. Among 3 aminoglycoside-resistant isolates, aac(3)-II was only detected in 1 isolate. All 8 APEC isolates with resistance to tetracycline carried the tetA gene. Overall, 6 of the 7 trimethoprim-sulfamethoxazole-resistant isolates carried the sul1 or sul2 genes, while only 2 of the 8 chloramphenicol-resistant isolates carried the catA1 gene. Although 9 isolates carried class I integrons, only 4 isolates carried the gene cassettes dfrA12-aadA2 (2 isolates), dfrA17-aadA5 (1 isolate), extX-psp-aadA2 (1 isolate), and dfrA27 (1 isolate). The most common plasmid replicon was FIB (8 isolates, 72.7%), followed by K/B (4 isolates, 36.4%). Antimicrobial resistance monitoring and molecular analysis of APEC should be performed continuously to surveil the transmission between poultry farms. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/32616259/Molecular_characteristics_of_fluoroquinolone-resistant_avian_pathogenic_Escherichia_coli_isolated_from_broiler_chickens L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(20)30189-9 DB - PRIME DP - Unbound Medicine ER -
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