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Web portal for analytical validation of MRM-MS assay abided with integrative multinational guidelines.
Sci Rep. 2020 Jul 02; 10(1):10848.SR

Abstract

Multiple reaction monitoring-mass spectrometry became a mainstream method for quantitative proteomics, which made the validation of a method and the analyzed data important. In this portal for validation of the MRM-MS assay, we developed a website that automatically evaluates uploaded MRM-MS data, based on biomarker assay guidelines from the European Medicines Agency, the US Food & Drug Administration, and the Korea Food & Drug Administration. The portal reads a Skyline output file and produces the following results-calibration curve, specificity, sensitivity, carryover, precision, recovery, matrix effect, recovery, dilution integrity, stability, and QC-according to the standards of each independent agency. The final tables and figures that pertain to the 11 evaluation categories are displayed in an individual page. Spring boot was used as a framework for development of the webpage, which follows MVC Pattern. JSP, HTML, XML, and Java Script were used to develop the webpage. A server was composed of Apache Tomcat, MySQL. Input files were skyline-derived output files (csv file), and each files were organized by specific columns in order. SQL, JAVA were interworked to evaluate all the categories and show the results. Method Validation Portal can be accessed via any kind of explorer from https://pnbvalid.snu.ac.kr.

Authors+Show Affiliations

Interdisciplinary Program of Bioengineering, Seoul National University College of Engineering, Seoul, South Korea.Interdisciplinary Program of Bioengineering, Seoul National University College of Engineering, Seoul, South Korea.Institute of Medical and Biological Engineering, MRC, Seoul National University, Seoul, South Korea.Institute of Medical and Biological Engineering, MRC, Seoul National University, Seoul, South Korea.Interdisciplinary Program of Bioengineering, Seoul National University College of Engineering, Seoul, South Korea.Interdisciplinary Program of Bioengineering, Seoul National University College of Engineering, Seoul, South Korea. biolab@snu.ac.kr. Institute of Medical and Biological Engineering, MRC, Seoul National University, Seoul, South Korea. biolab@snu.ac.kr. Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, South Korea. biolab@snu.ac.kr. Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, South Korea. biolab@snu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32616742

Citation

Kim, Jaenyeon, et al. "Web Portal for Analytical Validation of MRM-MS Assay Abided With Integrative Multinational Guidelines." Scientific Reports, vol. 10, no. 1, 2020, p. 10848.
Kim J, Yeo I, Kim H, et al. Web portal for analytical validation of MRM-MS assay abided with integrative multinational guidelines. Sci Rep. 2020;10(1):10848.
Kim, J., Yeo, I., Kim, H., Sohn, A., Kim, Y., & Kim, Y. (2020). Web portal for analytical validation of MRM-MS assay abided with integrative multinational guidelines. Scientific Reports, 10(1), 10848. https://doi.org/10.1038/s41598-020-67731-x
Kim J, et al. Web Portal for Analytical Validation of MRM-MS Assay Abided With Integrative Multinational Guidelines. Sci Rep. 2020 Jul 2;10(1):10848. PubMed PMID: 32616742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Web portal for analytical validation of MRM-MS assay abided with integrative multinational guidelines. AU - Kim,Jaenyeon, AU - Yeo,Injoon, AU - Kim,Hyunsoo, AU - Sohn,Areum, AU - Kim,Yoseop, AU - Kim,Youngsoo, Y1 - 2020/07/02/ PY - 2019/11/30/received PY - 2020/06/15/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline SP - 10848 EP - 10848 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - Multiple reaction monitoring-mass spectrometry became a mainstream method for quantitative proteomics, which made the validation of a method and the analyzed data important. In this portal for validation of the MRM-MS assay, we developed a website that automatically evaluates uploaded MRM-MS data, based on biomarker assay guidelines from the European Medicines Agency, the US Food & Drug Administration, and the Korea Food & Drug Administration. The portal reads a Skyline output file and produces the following results-calibration curve, specificity, sensitivity, carryover, precision, recovery, matrix effect, recovery, dilution integrity, stability, and QC-according to the standards of each independent agency. The final tables and figures that pertain to the 11 evaluation categories are displayed in an individual page. Spring boot was used as a framework for development of the webpage, which follows MVC Pattern. JSP, HTML, XML, and Java Script were used to develop the webpage. A server was composed of Apache Tomcat, MySQL. Input files were skyline-derived output files (csv file), and each files were organized by specific columns in order. SQL, JAVA were interworked to evaluate all the categories and show the results. Method Validation Portal can be accessed via any kind of explorer from https://pnbvalid.snu.ac.kr. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32616742/Web_portal_for_analytical_validation_of_MRM-MS_assay_abided_with_integrative_multinational_guidelines L2 - http://dx.doi.org/10.1038/s41598-020-67731-x DB - PRIME DP - Unbound Medicine ER -
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