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An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling.
Sci Rep. 2020 Jul 02; 10(1):10857.SR

Abstract

PHRF1 is involved in transforming growth factor β (TGF-β) signaling to constrain the formation of acute promyelocytic leukemia (APL) in mouse APL models. PHRF1 also participates in modulating non-homologous end-joining. However, the role of PHRF1 in mammalian dendrite architecture and synaptic plasticity is unclear. Here, we investigated the role of PHRF1 in dendritic formation in the murine hippocampus using Camk2a promoter driven-iCre recombinase to conduct a PHRF1 conditional knockout, namely PHRF1Δ/Δ, in the forebrain region. PHRF1Δ/Δ mice developed normally, but exhibited anxiety-like behaviors and displayed defective spatial memory. Alterations of dendritic complexity in apical and basal dendrites of pyramidal neurons were noticed in PHRF1Δ/Δ mutants. Furthermore, electrical stimulation in the hippocampal CA1 region after the TGF-β1 treatment showed a reduced synaptic plasticity in PHRF1Δ/Δ mice. Immunoblotting analysis indicated that PHRF1 ablation affected the TGF-β signaling. Collectively, our results demonstrate that PHRF1 is important for the dendritic architecture and required for spatial memory formation in the hippocampus.

Authors+Show Affiliations

Institute of Biochemical Sciences, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.Graduate Institute of Anatomy and Cell Biology, National Taiwan University, Taipei, Taiwan. Institute of Brain and Mind Sciences, National Taiwan University, Taipei, Taiwan. Neurobiology and Cognitive Science Center, National Taiwan University, Taipei, Taiwan.Graduate Institute of Anatomy and Cell Biology, National Taiwan University, Taipei, Taiwan.Institute of Biochemical Sciences, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.Institute of Biochemical Sciences, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan. mschang@ntu.edu.tw. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan. mschang@ntu.edu.tw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32616804

Citation

Shih, Ting-Wei, et al. "An Important Role of PHRF1 in Dendritic Architecture and Memory Formation By Modulating TGF-β Signaling." Scientific Reports, vol. 10, no. 1, 2020, p. 10857.
Shih TW, Lee LJ, Chang HC, et al. An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling. Sci Rep. 2020;10(1):10857.
Shih, T. W., Lee, L. J., Chang, H. C., Lin, H. W., & Chang, M. S. (2020). An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling. Scientific Reports, 10(1), 10857. https://doi.org/10.1038/s41598-020-67675-2
Shih TW, et al. An Important Role of PHRF1 in Dendritic Architecture and Memory Formation By Modulating TGF-β Signaling. Sci Rep. 2020 Jul 2;10(1):10857. PubMed PMID: 32616804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling. AU - Shih,Ting-Wei, AU - Lee,Li-Jen, AU - Chang,Ho-Ching, AU - Lin,Hung-Wei, AU - Chang,Mau-Sun, Y1 - 2020/07/02/ PY - 2019/11/21/received PY - 2020/06/02/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline SP - 10857 EP - 10857 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - PHRF1 is involved in transforming growth factor β (TGF-β) signaling to constrain the formation of acute promyelocytic leukemia (APL) in mouse APL models. PHRF1 also participates in modulating non-homologous end-joining. However, the role of PHRF1 in mammalian dendrite architecture and synaptic plasticity is unclear. Here, we investigated the role of PHRF1 in dendritic formation in the murine hippocampus using Camk2a promoter driven-iCre recombinase to conduct a PHRF1 conditional knockout, namely PHRF1Δ/Δ, in the forebrain region. PHRF1Δ/Δ mice developed normally, but exhibited anxiety-like behaviors and displayed defective spatial memory. Alterations of dendritic complexity in apical and basal dendrites of pyramidal neurons were noticed in PHRF1Δ/Δ mutants. Furthermore, electrical stimulation in the hippocampal CA1 region after the TGF-β1 treatment showed a reduced synaptic plasticity in PHRF1Δ/Δ mice. Immunoblotting analysis indicated that PHRF1 ablation affected the TGF-β signaling. Collectively, our results demonstrate that PHRF1 is important for the dendritic architecture and required for spatial memory formation in the hippocampus. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32616804/An_important_role_of_PHRF1_in_dendritic_architecture_and_memory_formation_by_modulating_TGF-β_signaling L2 - http://dx.doi.org/10.1038/s41598-020-67675-2 DB - PRIME DP - Unbound Medicine ER -
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