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Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica.
Sci Rep. 2020 07 02; 10(1):10866.SR

Abstract

The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a were expressed in most organs and could support whole plant basal auxin synthesis. MdYUCCA4a, MdYUCCA10b, and MdYUCCA11a expression indicated roles for these genes in auxin biosynthesis in vegetative organs. MdYUCCA2b, MdYUCCA11b, and MdYUCCA11d were mainly expressed in flower organs. High temperature induced the expression of MdYUCCA4a, MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a, and down-regulated the expression of MdYUCCA2b and MdYUCCA6b. Dual-luciferase assay indicated that MdPIF4 could trans-activate the MdYUCCA8a promoter. Overexpression of MdYUCCA8a increased IAA content, increased stem height, enhanced apical dominance, and led to silique malformation. These results provide a foundation for further investigation of the biological functions of apple MdYUCCAs.

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Authors+Show Affiliations

Song C
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China. College of Horticulture, Henan Agriculture University, Zhengzhou, Henan, 450002, China.
Zhang D
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Zheng L
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Shen Y
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Zuo X
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Mao J
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Meng Y
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Wu H
Gaotou Township People's Government of Wuji County, Shijiazhuang, Hebei, 050000, China.
Zhang Y
Taiyuan Academy of Agricultural Sciences, Taiyuan, Shannxi, 030000, China.
Liu X
Weinan Agro-Tech Extension Center, Weinan, Shaanxi, 714000, China.
Qian M
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Zhang J
Silkworm Tea Fruit Technology Extension Center in Nanzheng District, Hanzhong, 723100, Shaanxi, China.
Li G
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Zhao C
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Xing L
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Ma J
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Han M
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
An N
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China. anna206@nwsuaf.edu.cn. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China. anna206@nwsuaf.edu.cn.

MeSH

FlowersGene Expression ProfilingGene Expression Regulation, PlantGenome, PlantIndoleacetic AcidsMalusMultigene FamilyPhylogenyPlant DevelopmentPlant Growth RegulatorsPlant Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32616911

Citation

Song, Chunhui, et al. "Genome-wide Identification and Expression Profiling of the YUCCA Gene Family in Malus Domestica." Scientific Reports, vol. 10, no. 1, 2020, p. 10866.
Song C, Zhang D, Zheng L, et al. Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica. Sci Rep. 2020;10(1):10866.
Song, C., Zhang, D., Zheng, L., Shen, Y., Zuo, X., Mao, J., Meng, Y., Wu, H., Zhang, Y., Liu, X., Qian, M., Zhang, J., Li, G., Zhao, C., Xing, L., Ma, J., Han, M., & An, N. (2020). Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica. Scientific Reports, 10(1), 10866. https://doi.org/10.1038/s41598-020-66483-y
Song C, et al. Genome-wide Identification and Expression Profiling of the YUCCA Gene Family in Malus Domestica. Sci Rep. 2020 07 2;10(1):10866. PubMed PMID: 32616911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica. AU - Song,Chunhui, AU - Zhang,Dong, AU - Zheng,Liwei, AU - Shen,Yawen, AU - Zuo,Xiya, AU - Mao,Jiangping, AU - Meng,Yuan, AU - Wu,Haiqin, AU - Zhang,Yike, AU - Liu,Xiaoyuan, AU - Qian,Ming, AU - Zhang,Jie, AU - Li,Gaochao, AU - Zhao,Caiping, AU - Xing,Libo, AU - Ma,Juanjuan, AU - Han,Mingyu, AU - An,Na, Y1 - 2020/07/02/ PY - 2019/10/02/received PY - 2020/03/31/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/12/16/medline SP - 10866 EP - 10866 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a were expressed in most organs and could support whole plant basal auxin synthesis. MdYUCCA4a, MdYUCCA10b, and MdYUCCA11a expression indicated roles for these genes in auxin biosynthesis in vegetative organs. MdYUCCA2b, MdYUCCA11b, and MdYUCCA11d were mainly expressed in flower organs. High temperature induced the expression of MdYUCCA4a, MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a, and down-regulated the expression of MdYUCCA2b and MdYUCCA6b. Dual-luciferase assay indicated that MdPIF4 could trans-activate the MdYUCCA8a promoter. Overexpression of MdYUCCA8a increased IAA content, increased stem height, enhanced apical dominance, and led to silique malformation. These results provide a foundation for further investigation of the biological functions of apple MdYUCCAs. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32616911/Genome_wide_identification_and_expression_profiling_of_the_YUCCA_gene_family_in_Malus_domestica_ DB - PRIME DP - Unbound Medicine ER -