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Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines.
Evid Based Complement Alternat Med. 2020; 2020:2596082.EB

Abstract

Berberis lycium Royle has been traditionally used to cure rheumatism, eye and ear diseases, malarial fever, diabetes, stomach disorders, and skin diseases. There is a least amount of data available on cytotoxic capacity of Berberis lycium from Pakistani origin, so on this basis, the present study was aimed to screen Berberis lycium root bark extracts for cytotoxicity against cancer cell lines and isolation of chemical constituents from the most cytotoxic extract. Initial screening of extracts was performed on HepG2 cells at 100 μg/mL for 72 hours of treatment by using an MTT assay. Active fractions were subjected to a series of column chromatographies for the isolation of cytotoxic compounds. Molecular structures were elucidated by using combined data from 1H-NMR, 13C-NMR, and ESI-MS graphs. Assessment of reduction in cell proliferation by isolated compounds was performed on three human cancer cell lines (SK-Hep-1, HepG2, and NCI-H1299). Both n-hexane and chloroform fractions were found active with percent cell viabilities of 8.41 ± 2.23 and 22.31 ± 9.11 in HepG2 cells compared with lupeol 35.43 ± 3.35 percent viability. A protoberberine alkaloid identified as oxyberberine was isolated from chloroform fraction while β-sitosterol was isolated from n-hexane fraction. Oxyberberine inhibited SK-Hep-1 cell proliferation under a dose-dependent manner with an IC50 value of 34.26 ± 3.34 μM while HepG2 cells showed 50% inhibition at 62.96 ± 4.12 μM. β-Sitosterol showed reduction in cell viability in SK-Hep-1 cells and HepG2 cells with IC50 values of 123.12 ± 3.51 μM and 140 ± 4.21 μM. This is the first report on the isolation of oxyberberine and β-sitosterol from Berberis lycium root bark and their cytotoxic evaluation against SK-Hep-1 and NCI-H1299 cells. The cytotoxic potential of Berberis lycium Royle extracts and isolated compounds is suggesting that it is a promising candidate for anticancer drug discovery.

Authors+Show Affiliations

University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.University Institute of Biochemistry and Biotechnology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32617102

Citation

Anwar, Muhammad Asad, et al. "Isolation of Oxyberberine and β-Sitosterol From Berberis Lycium Royle Root Bark Extract and in Vitro Cytotoxicity Against Liver and Lung Cancer Cell Lines." Evidence-based Complementary and Alternative Medicine : ECAM, vol. 2020, 2020, p. 2596082.
Anwar MA, Tabassam S, Gulfraz M, et al. Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evid Based Complement Alternat Med. 2020;2020:2596082.
Anwar, M. A., Tabassam, S., Gulfraz, M., Sheeraz Ahmad, M., Raja, G. K., & Arshad, M. (2020). Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evidence-based Complementary and Alternative Medicine : ECAM, 2020, 2596082. https://doi.org/10.1155/2020/2596082
Anwar MA, et al. Isolation of Oxyberberine and β-Sitosterol From Berberis Lycium Royle Root Bark Extract and in Vitro Cytotoxicity Against Liver and Lung Cancer Cell Lines. Evid Based Complement Alternat Med. 2020;2020:2596082. PubMed PMID: 32617102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. AU - Anwar,Muhammad Asad, AU - Tabassam,Shahzadi, AU - Gulfraz,Muhammad, AU - Sheeraz Ahmad,Muhammad, AU - Raja,Ghazala Kaukab, AU - Arshad,Muhammad, Y1 - 2020/06/15/ PY - 2020/02/27/received PY - 2020/05/01/revised PY - 2020/05/20/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline SP - 2596082 EP - 2596082 JF - Evidence-based complementary and alternative medicine : eCAM JO - Evid Based Complement Alternat Med VL - 2020 N2 - Berberis lycium Royle has been traditionally used to cure rheumatism, eye and ear diseases, malarial fever, diabetes, stomach disorders, and skin diseases. There is a least amount of data available on cytotoxic capacity of Berberis lycium from Pakistani origin, so on this basis, the present study was aimed to screen Berberis lycium root bark extracts for cytotoxicity against cancer cell lines and isolation of chemical constituents from the most cytotoxic extract. Initial screening of extracts was performed on HepG2 cells at 100 μg/mL for 72 hours of treatment by using an MTT assay. Active fractions were subjected to a series of column chromatographies for the isolation of cytotoxic compounds. Molecular structures were elucidated by using combined data from 1H-NMR, 13C-NMR, and ESI-MS graphs. Assessment of reduction in cell proliferation by isolated compounds was performed on three human cancer cell lines (SK-Hep-1, HepG2, and NCI-H1299). Both n-hexane and chloroform fractions were found active with percent cell viabilities of 8.41 ± 2.23 and 22.31 ± 9.11 in HepG2 cells compared with lupeol 35.43 ± 3.35 percent viability. A protoberberine alkaloid identified as oxyberberine was isolated from chloroform fraction while β-sitosterol was isolated from n-hexane fraction. Oxyberberine inhibited SK-Hep-1 cell proliferation under a dose-dependent manner with an IC50 value of 34.26 ± 3.34 μM while HepG2 cells showed 50% inhibition at 62.96 ± 4.12 μM. β-Sitosterol showed reduction in cell viability in SK-Hep-1 cells and HepG2 cells with IC50 values of 123.12 ± 3.51 μM and 140 ± 4.21 μM. This is the first report on the isolation of oxyberberine and β-sitosterol from Berberis lycium root bark and their cytotoxic evaluation against SK-Hep-1 and NCI-H1299 cells. The cytotoxic potential of Berberis lycium Royle extracts and isolated compounds is suggesting that it is a promising candidate for anticancer drug discovery. SN - 1741-427X UR - https://www.unboundmedicine.com/medline/citation/32617102/Isolation_of_Oxyberberine_and_β-Sitosterol_from_Berberis_lycium_Royle_Root_Bark_Extract_and_In_Vitro_Cytotoxicity_against_Liver_and_Lung_Cancer_Cell_Lines DB - PRIME DP - Unbound Medicine ER -
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