Tags

Type your tag names separated by a space and hit enter

Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids.
Microb Ecol. 2020 Jul 02 [Online ahead of print]ME

Abstract

Metagenomic studies revealed the prevalence of Acidobacteria in soils, but the physiological and ecological reasons for their success are not well understood. Many Acidobacteria exhibit carotenoid-related pigments, which may be involved in their tolerance of environmental stress. The aim of this work was to investigate the role of the orange pigments produced by Acidobacteria strain AB23 isolated from a savannah-like soil and to identify putative carotenoid genes in Acidobacteria genomes. Phylogenetic analysis revealed that strain AB23 belongs to the Occallatibacter genus from the class Acidobacteriia (subdivision 1). Strain AB23 produced carotenoids in the presence of light and vitamins; however, the growth rate and biomass decreased when cells were exposed to light. The presence of carotenoids resulted in tolerance to hydrogen peroxide. Comparative genomics revealed that all members of Acidobacteriia with available genomes possess the complete gene cluster for phytoene production. Some Acidobacteriia members have an additional gene cluster that may be involved in the production of colored carotenoids. Both colored and colorless carotenoids are involved in tolerance to oxidative stress. These results show that the presence of carotenoid genes is widespread among Acidobacteriia. Light and atmospheric oxygen stimulate carotenoid synthesis, but there are other natural sources of oxidative stress in soils. Tolerance to environmental oxidative stress provided by carotenoids may offer a competitive advantage for Acidobacteria in soils.

Authors+Show Affiliations

Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, SGAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, 70790-160, Brazil. Laboratory of Enzymology, Institute of Biological Sciences, Department of Cell Biology, University of Brasília, Brasília, 70910-900, Brazil.Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, SGAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, 70790-160, Brazil. Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743, Jena, Germany.Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, SGAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, 70790-160, Brazil.Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, SGAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, 70790-160, Brazil.Research Center René Rachou, Oswaldo Cruz Foundation (Fiocruz), Avenida Augusto de Lima 1715, Barro Preto, Belo Horizonte, 30190-002, Brazil.Department of Physiological Sciences, Institute of Biological Sciences, Universidade de Brasília, Brasília, DF, 70910-900, Brazil.Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, SGAN 916 Módulo B Avenida W5 - Asa Norte, Brasília, 70790-160, Brazil. criscbarreto@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32617619

Citation

Pinto, Otávio Henrique Bezerra, et al. "Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids." Microbial Ecology, 2020.
Pinto OHB, Costa FS, Rodrigues GR, et al. Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids. Microb Ecol. 2020.
Pinto, O. H. B., Costa, F. S., Rodrigues, G. R., da Costa, R. A., da Rocha Fernandes, G., Júnior, O. R. P., & Barreto, C. C. (2020). Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids. Microbial Ecology. https://doi.org/10.1007/s00248-020-01548-z
Pinto OHB, et al. Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids. Microb Ecol. 2020 Jul 2; PubMed PMID: 32617619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids. AU - Pinto,Otávio Henrique Bezerra, AU - Costa,Flávio Silva, AU - Rodrigues,Gisele Regina, AU - da Costa,Rosiane Andrade, AU - da Rocha Fernandes,Gabriel, AU - Júnior,Osmindo Rodrigues Pires, AU - Barreto,Cristine Chaves, Y1 - 2020/07/02/ PY - 2020/01/22/received PY - 2020/06/16/accepted PY - 2020/7/4/entrez KW - Acidobacteria genome KW - Carotenoids KW - Occallatibacter KW - Oxidative stress KW - Pigments JF - Microbial ecology JO - Microb. Ecol. N2 - Metagenomic studies revealed the prevalence of Acidobacteria in soils, but the physiological and ecological reasons for their success are not well understood. Many Acidobacteria exhibit carotenoid-related pigments, which may be involved in their tolerance of environmental stress. The aim of this work was to investigate the role of the orange pigments produced by Acidobacteria strain AB23 isolated from a savannah-like soil and to identify putative carotenoid genes in Acidobacteria genomes. Phylogenetic analysis revealed that strain AB23 belongs to the Occallatibacter genus from the class Acidobacteriia (subdivision 1). Strain AB23 produced carotenoids in the presence of light and vitamins; however, the growth rate and biomass decreased when cells were exposed to light. The presence of carotenoids resulted in tolerance to hydrogen peroxide. Comparative genomics revealed that all members of Acidobacteriia with available genomes possess the complete gene cluster for phytoene production. Some Acidobacteriia members have an additional gene cluster that may be involved in the production of colored carotenoids. Both colored and colorless carotenoids are involved in tolerance to oxidative stress. These results show that the presence of carotenoid genes is widespread among Acidobacteriia. Light and atmospheric oxygen stimulate carotenoid synthesis, but there are other natural sources of oxidative stress in soils. Tolerance to environmental oxidative stress provided by carotenoids may offer a competitive advantage for Acidobacteria in soils. SN - 1432-184X UR - https://www.unboundmedicine.com/medline/citation/32617619/Soil_Acidobacteria_Strain_AB23_Resistance_to_Oxidative_Stress_Through_Production_of_Carotenoids L2 - https://dx.doi.org/10.1007/s00248-020-01548-z DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.