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Anti-Sporothrix activity of ibuprofen combined with antifungal.
Braz J Microbiol. 2020 Jul 02 [Online ahead of print]BJ

Abstract

The in vitro activity of ibuprofen, a nonsteroidal anti-inflammatory drug, was evaluated against Sporothrix brasiliensis and S. schenckii, either alone or in combination with amphotericin B, itraconazole, or terbinafine. The inhibitory activity of ibuprofen as a single agent was determined according to minimum inhibitory concentration (MIC) values, while the effect of ibuprofen combined with amphotericin B, itraconazole, or terbinafine was estimated by microdilution checkerboard methodology. The ultrastructural alterations of S. schenckii after exposure to the combination of ibuprofen and amphotericin B were evaluated by scanning electron microscopy (SEM) and flow cytometry analysis. As a single agent, ibuprofen inhibited Sporothrix growth with a MIC median of 256 μg/mL, while the MIC medians of ibuprofen in combination with antifungals were 16 μg/mL and 128 μg/mL. The MIC values of amphotericin B, itraconazole, and terbinafine were reduced when isolates were co-incubated with ibuprofen, mainly the polyene. The major alteration after treatment with the ibuprofen/amphotericin B combination was the increase in the presence of filamentous forms and high membrane damage with loss of plasma membrane integrity. In summary, we demonstrated that ibuprofen increases the in vitro activity of antifungals, mainly amphotericin B, against S. brasiliensis and S. schenckii. Future in vivo studies exploring combination therapy with ibuprofen and antifungals in animal models are needed to confirm its efficacy.

Authors+Show Affiliations

Laboratório de Biologia Celular de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Centro de Ciências da Saúde, Bloco G, Sala G0-044, Rio de Janeiro, RJ, 21941-902, Brazil. luanaborba@biof.ufrj.br.Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Microbiologia Paulo de Goés, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Laboratório de Biologia Celular de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Centro de Ciências da Saúde, Bloco G, Sala G0-044, Rio de Janeiro, RJ, 21941-902, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32617835

Citation

Borba-Santos, Luana Pereira, et al. "Anti-Sporothrix Activity of Ibuprofen Combined With Antifungal." Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], 2020.
Borba-Santos LP, Nucci M, Ferreira-Pereira A, et al. Anti-Sporothrix activity of ibuprofen combined with antifungal. Braz J Microbiol. 2020.
Borba-Santos, L. P., Nucci, M., Ferreira-Pereira, A., & Rozental, S. (2020). Anti-Sporothrix activity of ibuprofen combined with antifungal. Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]. https://doi.org/10.1007/s42770-020-00327-9
Borba-Santos LP, et al. Anti-Sporothrix Activity of Ibuprofen Combined With Antifungal. Braz J Microbiol. 2020 Jul 2; PubMed PMID: 32617835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-Sporothrix activity of ibuprofen combined with antifungal. AU - Borba-Santos,Luana Pereira, AU - Nucci,Marcio, AU - Ferreira-Pereira,Antonio, AU - Rozental,Sonia, Y1 - 2020/07/02/ PY - 2019/11/10/received PY - 2020/06/25/accepted PY - 2020/7/4/entrez KW - Amphotericin B KW - Ibuprofen KW - Sporothrix brasiliensis KW - Sporothrix schenckii JF - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] JO - Braz. J. Microbiol. N2 - The in vitro activity of ibuprofen, a nonsteroidal anti-inflammatory drug, was evaluated against Sporothrix brasiliensis and S. schenckii, either alone or in combination with amphotericin B, itraconazole, or terbinafine. The inhibitory activity of ibuprofen as a single agent was determined according to minimum inhibitory concentration (MIC) values, while the effect of ibuprofen combined with amphotericin B, itraconazole, or terbinafine was estimated by microdilution checkerboard methodology. The ultrastructural alterations of S. schenckii after exposure to the combination of ibuprofen and amphotericin B were evaluated by scanning electron microscopy (SEM) and flow cytometry analysis. As a single agent, ibuprofen inhibited Sporothrix growth with a MIC median of 256 μg/mL, while the MIC medians of ibuprofen in combination with antifungals were 16 μg/mL and 128 μg/mL. The MIC values of amphotericin B, itraconazole, and terbinafine were reduced when isolates were co-incubated with ibuprofen, mainly the polyene. The major alteration after treatment with the ibuprofen/amphotericin B combination was the increase in the presence of filamentous forms and high membrane damage with loss of plasma membrane integrity. In summary, we demonstrated that ibuprofen increases the in vitro activity of antifungals, mainly amphotericin B, against S. brasiliensis and S. schenckii. Future in vivo studies exploring combination therapy with ibuprofen and antifungals in animal models are needed to confirm its efficacy. SN - 1678-4405 UR - https://www.unboundmedicine.com/medline/citation/32617835/Anti-Sporothrix_activity_of_ibuprofen_combined_with_antifungal L2 - https://dx.doi.org/10.1007/s42770-020-00327-9 DB - PRIME DP - Unbound Medicine ER -
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