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Expression of Cell Wall-Modifying Enzymes in Aspen for Improved Lignocellulose Processing.
Methods Mol Biol. 2020; 2149:145-164.MM

Abstract

Wood is an important source of biomass for materials and chemicals, and a target for genetic engineering of its properties for different applications or for research. Wood properties can be altered by using different enzymes acting on cell wall polymers postsynthetically in cell walls. This approach allows for a precise polymer structure modification thanks to the specificity of enzymes used. Such enzymes can originate from all kinds of organisms, or even be modified in a desired way for novel attributes. Here we present a general strategy for expressing a microbial enzyme in aspen and targeting it to cell wall, using an example of fungal glucuronoyl esterase. We describe methods of vector cloning, plant transformation, transgenic line selection and multiplication, testing for the presence of enzymatic activity in different cell compartments, and finally the method of plant transferring from sterile culture to the greenhouse conditions.

Authors+Show Affiliations

Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden. marta.derba-maceluch@slu.se.Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32617934

Citation

Derba-Maceluch, Marta, and Ewa J. Mellerowicz. "Expression of Cell Wall-Modifying Enzymes in Aspen for Improved Lignocellulose Processing." Methods in Molecular Biology (Clifton, N.J.), vol. 2149, 2020, pp. 145-164.
Derba-Maceluch M, Mellerowicz EJ. Expression of Cell Wall-Modifying Enzymes in Aspen for Improved Lignocellulose Processing. Methods Mol Biol. 2020;2149:145-164.
Derba-Maceluch, M., & Mellerowicz, E. J. (2020). Expression of Cell Wall-Modifying Enzymes in Aspen for Improved Lignocellulose Processing. Methods in Molecular Biology (Clifton, N.J.), 2149, 145-164. https://doi.org/10.1007/978-1-0716-0621-6_9
Derba-Maceluch M, Mellerowicz EJ. Expression of Cell Wall-Modifying Enzymes in Aspen for Improved Lignocellulose Processing. Methods Mol Biol. 2020;2149:145-164. PubMed PMID: 32617934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Cell Wall-Modifying Enzymes in Aspen for Improved Lignocellulose Processing. AU - Derba-Maceluch,Marta, AU - Mellerowicz,Ewa J, PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - Aspen transformation KW - Fungal wood degrading enzymes KW - Gateway® cloning KW - In vitro culture KW - Populus tremula × tremuloides KW - Protein targeting to cell wall KW - Transgenic trees KW - Vector design SP - 145 EP - 164 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol. Biol. VL - 2149 N2 - Wood is an important source of biomass for materials and chemicals, and a target for genetic engineering of its properties for different applications or for research. Wood properties can be altered by using different enzymes acting on cell wall polymers postsynthetically in cell walls. This approach allows for a precise polymer structure modification thanks to the specificity of enzymes used. Such enzymes can originate from all kinds of organisms, or even be modified in a desired way for novel attributes. Here we present a general strategy for expressing a microbial enzyme in aspen and targeting it to cell wall, using an example of fungal glucuronoyl esterase. We describe methods of vector cloning, plant transformation, transgenic line selection and multiplication, testing for the presence of enzymatic activity in different cell compartments, and finally the method of plant transferring from sterile culture to the greenhouse conditions. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/32617934/Expression_of_Cell_Wall-Modifying_Enzymes_in_Aspen_for_Improved_Lignocellulose_Processing L2 - https://dx.doi.org/10.1007/978-1-0716-0621-6_9 DB - PRIME DP - Unbound Medicine ER -
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