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Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer.
J Cell Physiol. 2020 Jul 02 [Online ahead of print]JC

Abstract

We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalization indicating that its membrane location requires Dab2. In mice, we found that 3 days of dextran sulfate sodium-induced colitis increased Dab2/E-cadherin colocalization, which was decreased as colitis progressed to 6 and 9 days. In agreement, Dab2/E-cadherin colocalization increased in human mild and severe ulcerative colitis and in polyps, being reduced in colon adenocarcinomas, which even showed epithelial Dab2 absence and E-cadherin delocalization. Epithelial Dab2 decrement preceded that of E-cadherin. We suggest that Dab2, by inhibiting E-cadherin internalization, stabilizes adherens junctions, and its absence from the epithelium may contribute to development of colon inflammation and cancer.

Authors+Show Affiliations

Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.Department of Cell Biology, Institute of Ophthalmology, University College London, London, UK.Department of Cell Biology, Institute of Ophthalmology, University College London, London, UK.Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32617970

Citation

Vazquez-Carretero, María D., et al. "Proper E-cadherin Membrane Location in Colon Requires Dab2 and It Modifies By Inflammation and Cancer." Journal of Cellular Physiology, 2020.
Vazquez-Carretero MD, García-Miranda P, Balda MS, et al. Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer. J Cell Physiol. 2020.
Vazquez-Carretero, M. D., García-Miranda, P., Balda, M. S., Matter, K., Ilundáin, A. A., & Peral, M. J. (2020). Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer. Journal of Cellular Physiology. https://doi.org/10.1002/jcp.29917
Vazquez-Carretero MD, et al. Proper E-cadherin Membrane Location in Colon Requires Dab2 and It Modifies By Inflammation and Cancer. J Cell Physiol. 2020 Jul 2; PubMed PMID: 32617970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer. AU - Vazquez-Carretero,María D, AU - García-Miranda,Pablo, AU - Balda,María S, AU - Matter,Karl, AU - Ilundáin,Anunciación A, AU - Peral,María J, Y1 - 2020/07/02/ PY - 2020/03/23/received PY - 2020/06/03/revised PY - 2020/06/21/accepted PY - 2020/7/4/pubmed PY - 2020/7/4/medline PY - 2020/7/4/entrez KW - Dab2 KW - E-cadherin KW - cancer KW - colon KW - inflammation KW - junctions JF - Journal of cellular physiology JO - J. Cell. Physiol. N2 - We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalization indicating that its membrane location requires Dab2. In mice, we found that 3 days of dextran sulfate sodium-induced colitis increased Dab2/E-cadherin colocalization, which was decreased as colitis progressed to 6 and 9 days. In agreement, Dab2/E-cadherin colocalization increased in human mild and severe ulcerative colitis and in polyps, being reduced in colon adenocarcinomas, which even showed epithelial Dab2 absence and E-cadherin delocalization. Epithelial Dab2 decrement preceded that of E-cadherin. We suggest that Dab2, by inhibiting E-cadherin internalization, stabilizes adherens junctions, and its absence from the epithelium may contribute to development of colon inflammation and cancer. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/32617970/Proper_E-cadherin_membrane_location_in_colon_requires_Dab2_and_it_modifies_by_inflammation_and_cancer L2 - https://doi.org/10.1002/jcp.29917 DB - PRIME DP - Unbound Medicine ER -
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