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Isolation and experimental challenge of cultured burbot (Lota lota maculosa) with Flavobacterium columnare and Aeromonas sp. isolates.
J Fish Dis. 2020 Aug; 43(8):839-851.JF

Abstract

Burbot (Lota lota maculosa) are a potential new species for commercial aquaculture. As burbot culture expands, there is a need to further define pathogen susceptibility and characterize aspects of the burbot immune response in an effort to assess fish health. A recent clinical diagnostic case from juvenile burbot reared at a commercial production facility resulted in the isolation and identification of Flavobacterium columnare along with several Aeromonas spp. The F. columnare isolate was assigned to genetic group 1 via multiplex PCR, a genetic group commonly associated with columnaris disease cases in rainbow trout (Oncorhynchus mykiss). Virulence of the F. columnare isolate was assessed in vivo in both juvenile burbot and rainbow trout. Additionally, several of the Aeromonas sp. case isolates were identified via sequencing (16S rRNA, gyrB and rpoD) and a putative A. sobria isolate (BI-3) was used to challenge burbot, along with a known virulent Aeromonas sp. (A141), but BI-3 was not found to be virulent. Burbot were refractory to F. columnare when challenged by immersion, and it is likely that this is a secondary pathogen for burbot. Although refractory in burbot, the identified F. columnare isolate (BI-1) was found to be virulent in rainbow trout.

Authors+Show Affiliations

Department of Fish and Wildlife Sciences, University of Idaho, Moscow, ID, USA.Department of Fish and Wildlife Sciences, University of Idaho, Moscow, ID, USA.Department of Fish and Wildlife Sciences, University of Idaho, Moscow, ID, USA.Department of Fish and Wildlife Sciences, University of Idaho, Moscow, ID, USA.USDA-ARS Aquatic Animal Health Research Unit, Auburn, AL, USA.Department of Fish and Wildlife Sciences, University of Idaho, Moscow, ID, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32618015

Citation

Bruce, Timothy J., et al. "Isolation and Experimental Challenge of Cultured Burbot (Lota Lota Maculosa) With Flavobacterium Columnare and Aeromonas Sp. Isolates." Journal of Fish Diseases, vol. 43, no. 8, 2020, pp. 839-851.
Bruce TJ, Ma J, Oliver LP, et al. Isolation and experimental challenge of cultured burbot (Lota lota maculosa) with Flavobacterium columnare and Aeromonas sp. isolates. J Fish Dis. 2020;43(8):839-851.
Bruce, T. J., Ma, J., Oliver, L. P., Jones, E. M., LaFrentz, B. R., & Cain, K. D. (2020). Isolation and experimental challenge of cultured burbot (Lota lota maculosa) with Flavobacterium columnare and Aeromonas sp. isolates. Journal of Fish Diseases, 43(8), 839-851. https://doi.org/10.1111/jfd.13169
Bruce TJ, et al. Isolation and Experimental Challenge of Cultured Burbot (Lota Lota Maculosa) With Flavobacterium Columnare and Aeromonas Sp. Isolates. J Fish Dis. 2020;43(8):839-851. PubMed PMID: 32618015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and experimental challenge of cultured burbot (Lota lota maculosa) with Flavobacterium columnare and Aeromonas sp. isolates. AU - Bruce,Timothy J, AU - Ma,Jie, AU - Oliver,Luke P, AU - Jones,Evan M, AU - LaFrentz,Benjamin R, AU - Cain,Kenneth D, Y1 - 2020/07/02/ PY - 2020/01/07/received PY - 2020/03/19/revised PY - 2020/03/20/accepted PY - 2020/7/4/pubmed PY - 2020/7/4/medline PY - 2020/7/4/entrez KW - Aeromonas KW - Flavobacterium KW - burbot KW - diagnostics KW - fish pathology SP - 839 EP - 851 JF - Journal of fish diseases JO - J. Fish Dis. VL - 43 IS - 8 N2 - Burbot (Lota lota maculosa) are a potential new species for commercial aquaculture. As burbot culture expands, there is a need to further define pathogen susceptibility and characterize aspects of the burbot immune response in an effort to assess fish health. A recent clinical diagnostic case from juvenile burbot reared at a commercial production facility resulted in the isolation and identification of Flavobacterium columnare along with several Aeromonas spp. The F. columnare isolate was assigned to genetic group 1 via multiplex PCR, a genetic group commonly associated with columnaris disease cases in rainbow trout (Oncorhynchus mykiss). Virulence of the F. columnare isolate was assessed in vivo in both juvenile burbot and rainbow trout. Additionally, several of the Aeromonas sp. case isolates were identified via sequencing (16S rRNA, gyrB and rpoD) and a putative A. sobria isolate (BI-3) was used to challenge burbot, along with a known virulent Aeromonas sp. (A141), but BI-3 was not found to be virulent. Burbot were refractory to F. columnare when challenged by immersion, and it is likely that this is a secondary pathogen for burbot. Although refractory in burbot, the identified F. columnare isolate (BI-1) was found to be virulent in rainbow trout. SN - 1365-2761 UR - https://www.unboundmedicine.com/medline/citation/32618015/Isolation_and_experimental_challenge_of_cultured_burbot_(Lota_lota_maculosa)_with_Flavobacterium_columnare_and_Aeromonas_sp._isolates L2 - https://doi.org/10.1111/jfd.13169 DB - PRIME DP - Unbound Medicine ER -
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