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The Leaf Extracts of Toona sinensis and Fermented Culture Broths of Antrodia camphorata Synergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells.
Integr Cancer Ther. 2020 Jan-Dec; 19:1534735420923734.IC

Abstract

Toona sinensis is a common edible vegetable that is used in certain Chinese dishes and has importance in folk medicine. The leaf extracts of T sinensis possess and exhibit anticancer efficacy against various cancer cell types. In Taiwanese folklore, Antrodia camphorata, also known as "Niu-Cheng-Zi," is used in traditional medicine to treat various illnesses. Its fruit and mycelium possess various potent antiproliferative properties. Two studies from our group have reported that T sinensis or A camphorata has the ability to cause apoptosis in various cancer cells. Conversely, underlying molecular mechanisms and any beneficial effects remain unknown. This study shows anticancer efficacy for both T sinensis and A camphorata co-treatments that target HL-60 cells. The combination index values indicate that 40 µg/mL of T sinensis and 25 µg/mL of A camphorata as a combined treatment shows a synergetic effect, which reduces HL-60 cell proliferation. Alternately, this treatment exhibited no cytotoxic effects for human umbilical vein endothelial cells. Western blot data showed that T sinensis and A camphorata as a combined treatment result in augmented expression of apoptosis, cytochrome c release, Bcl-2 inhibition, expression of Bax, Fas, and FasL, as well as the cleavage of Bid in HL-60 cells. Moreover, this combined treatment overshadowed monotherapy in its ability to inhibit uPAR, MMP-9, MMP-2, COX-2 expression, and PGE2 secretions. Our study strongly implies that this combined treatment offers more beneficial effects to suppress and treat leukemia due to apoptosis-mediated cell inhibition. Further in vivo studies related to the combined treatment could establish its future potential.

Authors+Show Affiliations

Department of Nutrition, China Medical University, Taichung, Taiwan.Department of Nutrition, China Medical University, Taichung, Taiwan.Department of Cosmeceutics, School of Pharmacy, China Medical University, Taichung, Taiwan.Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.School of Pharmacy, China Medical University, Taichung, Taiwan.Department of Cosmeceutics, School of Pharmacy, China Medical University, Taichung, Taiwan. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan. Chinese Medicine Research Center, China Medical University, Taichung, Taiwan. Research Center of Chinese Herbal Medicine, China Medical University, Taichung, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32618215

Citation

Yang, Hsin-Ling, et al. "The Leaf Extracts of Toona Sinensis and Fermented Culture Broths of Antrodia Camphorata Synergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells." Integrative Cancer Therapies, vol. 19, 2020, p. 1534735420923734.
Yang HL, Kuo YT, Vudhya Gowrisankar Y, et al. The Leaf Extracts of Toona sinensis and Fermented Culture Broths of Antrodia camphorata Synergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells. Integr Cancer Ther. 2020;19:1534735420923734.
Yang, H. L., Kuo, Y. T., Vudhya Gowrisankar, Y., Lin, K. Y., Hsu, L. S., Huang, P. J., Lin, H. C., & Hseu, Y. C. (2020). The Leaf Extracts of Toona sinensis and Fermented Culture Broths of Antrodia camphorata Synergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells. Integrative Cancer Therapies, 19, 1534735420923734. https://doi.org/10.1177/1534735420923734
Yang HL, et al. The Leaf Extracts of Toona Sinensis and Fermented Culture Broths of Antrodia Camphorata Synergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells. Integr Cancer Ther. 2020 Jan-Dec;19:1534735420923734. PubMed PMID: 32618215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Leaf Extracts of Toona sinensis and Fermented Culture Broths of Antrodia camphorata Synergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells. AU - Yang,Hsin-Ling, AU - Kuo,Ya-Ting, AU - Vudhya Gowrisankar,Yugandhar, AU - Lin,Kai-Yuan, AU - Hsu,Li-Sung, AU - Huang,Pei-Jane, AU - Lin,Hui-Chang, AU - Hseu,You-Cheng, PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - Antrodia camphorate KW - COX-2 KW - HL-60 cells KW - MMP KW - Toona sinensis KW - apoptosis KW - leukemia SP - 1534735420923734 EP - 1534735420923734 JF - Integrative cancer therapies JO - Integr Cancer Ther VL - 19 N2 - Toona sinensis is a common edible vegetable that is used in certain Chinese dishes and has importance in folk medicine. The leaf extracts of T sinensis possess and exhibit anticancer efficacy against various cancer cell types. In Taiwanese folklore, Antrodia camphorata, also known as "Niu-Cheng-Zi," is used in traditional medicine to treat various illnesses. Its fruit and mycelium possess various potent antiproliferative properties. Two studies from our group have reported that T sinensis or A camphorata has the ability to cause apoptosis in various cancer cells. Conversely, underlying molecular mechanisms and any beneficial effects remain unknown. This study shows anticancer efficacy for both T sinensis and A camphorata co-treatments that target HL-60 cells. The combination index values indicate that 40 µg/mL of T sinensis and 25 µg/mL of A camphorata as a combined treatment shows a synergetic effect, which reduces HL-60 cell proliferation. Alternately, this treatment exhibited no cytotoxic effects for human umbilical vein endothelial cells. Western blot data showed that T sinensis and A camphorata as a combined treatment result in augmented expression of apoptosis, cytochrome c release, Bcl-2 inhibition, expression of Bax, Fas, and FasL, as well as the cleavage of Bid in HL-60 cells. Moreover, this combined treatment overshadowed monotherapy in its ability to inhibit uPAR, MMP-9, MMP-2, COX-2 expression, and PGE2 secretions. Our study strongly implies that this combined treatment offers more beneficial effects to suppress and treat leukemia due to apoptosis-mediated cell inhibition. Further in vivo studies related to the combined treatment could establish its future potential. SN - 1552-695X UR - https://www.unboundmedicine.com/medline/citation/32618215/The_Leaf_Extracts_of_Toona_sinensis_and_Fermented_Culture_Broths_of_Antrodia_camphorata_Synergistically_Cause_Apoptotic_Cell_Death_in_Promyelocytic_Leukemia_Cells L2 - https://journals.sagepub.com/doi/10.1177/1534735420923734?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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