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Isolation and maintenance of Batrachochytrium salamandrivorans cultures.
Dis Aquat Organ. 2020 Jun 18; 140:1-11.DA

Abstract

Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue.

Authors+Show Affiliations

Department of Biology, University of Massachusetts, Amherst, MA 01003, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32618283

Citation

Robinson, Kristyn A., et al. "Isolation and Maintenance of Batrachochytrium Salamandrivorans Cultures." Diseases of Aquatic Organisms, vol. 140, 2020, pp. 1-11.
Robinson KA, Pereira KE, Bletz MC, et al. Isolation and maintenance of Batrachochytrium salamandrivorans cultures. Dis Aquat Org. 2020;140:1-11.
Robinson, K. A., Pereira, K. E., Bletz, M. C., Carter, E. D., Gray, M. J., Piovia-Scott, J., Romansic, J. M., Woodhams, D. C., & Fritz-Laylin, L. (2020). Isolation and maintenance of Batrachochytrium salamandrivorans cultures. Diseases of Aquatic Organisms, 140, 1-11. https://doi.org/10.3354/dao03488
Robinson KA, et al. Isolation and Maintenance of Batrachochytrium Salamandrivorans Cultures. Dis Aquat Org. 2020 Jun 18;140:1-11. PubMed PMID: 32618283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and maintenance of Batrachochytrium salamandrivorans cultures. AU - Robinson,Kristyn A, AU - Pereira,Kenzie E, AU - Bletz,Molly C, AU - Carter,Edward Davis, AU - Gray,Matthew J, AU - Piovia-Scott,Jonah, AU - Romansic,John M, AU - Woodhams,Douglas C, AU - Fritz-Laylin,Lillian, Y1 - 2020/06/18/ PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/14/medline KW - Amphibian disease KW - Chytridiomycosis KW - Emerging infectious disease KW - Life cycle KW - Management SP - 1 EP - 11 JF - Diseases of aquatic organisms JO - Dis. Aquat. Org. VL - 140 N2 - Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue. SN - 0177-5103 UR - https://www.unboundmedicine.com/medline/citation/32618283/Isolation_and_maintenance_of_Batrachochytrium_salamandrivorans_cultures L2 - https://doi.org/10.3354/dao03488 DB - PRIME DP - Unbound Medicine ER -