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Peptides in Blood, Urine and Dialysate: Towards Unravelling Renal Peptide Handling.
Proteomics Clin Appl. 2020 Jul 03 [Online ahead of print]PC

Abstract

PURPOSE

We investigated the peptidomes of spent hemodialysate, urine, and plasma, to shed light on peptide handling in the kidney.

EXPERIMENTAL DESIGN

We collected 15 plasma, 15 urine and 13 spent hemodialysate samples from age and sex-matched subjects with chronic kidney disease. Peptide identification and quantification were performed with capillary electrophoresis-coupled mass spectrometry.

RESULTS

We detected 6278 urinary peptides, 1743 plasma peptides and 1727 peptides from spent hemodialysate. Of these, we could assign sequences to 1580, 419 and 352 peptides respectively. We found a strong correlation in peptide abundance between urine and spent hemodialysate (P = 3e-21, Rho = 0.52), a moderately strong correlation between spent hemodialysate and plasma (P = 4.5e-5, Rho = 0.30), and no significant correlation between urine and plasma (P = 0.11, Rho = 0.094). Collagen and fibrinogen alpha peptides are highly abundant in all three body fluids. In spent hemodialysate, thymosin β4 is one of the most abundant peptides, which is shown to be negatively associated with the estimated glomerular filtration rate (Rho = -0.39, p-value = 3.9e-81).

CONCLUSION AND CLINICAL RELEVANCE

The correlation of peptide abundance in these three body fluids is lower than expected, supporting the hypothesis that tubular reabsorption has a major impact on urinary peptide content. Further investigation of thymosin β4 in hemodialysis is thus warranted. This article is protected by copyright. All rights reserved.

Authors+Show Affiliations

T. He, M. Pejchinovski, H. Mischak, Mosaiques Diagnostics GmbH, Hannover, Germany. T. He, V. Jankowski, Institute for Molecular Cardiovascular Research (IMCAR), University of Aachen, Weinheim, Germany.T. He, M. Pejchinovski, H. Mischak, Mosaiques Diagnostics GmbH, Hannover, Germany.W. Mullen, H. Mischak, Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow, UK.J. Beige, Department of Nephrology and Kuratorium for Dialysis and Transplantation (KfH) Renal Unit, Hospital St. Georg, Leipzig, Germany.T. He, M. Pejchinovski, H. Mischak, Mosaiques Diagnostics GmbH, Hannover, Germany. W. Mullen, H. Mischak, Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow, UK.T. He, V. Jankowski, Institute for Molecular Cardiovascular Research (IMCAR), University of Aachen, Weinheim, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32618437

Citation

He, Tianlin, et al. "Peptides in Blood, Urine and Dialysate: Towards Unravelling Renal Peptide Handling." Proteomics. Clinical Applications, 2020, pp. e2000029.
He T, Pejchinovski M, Mullen W, et al. Peptides in Blood, Urine and Dialysate: Towards Unravelling Renal Peptide Handling. Proteomics Clin Appl. 2020.
He, T., Pejchinovski, M., Mullen, W., Beige, J., Mischak, H., & Jankowski, V. (2020). Peptides in Blood, Urine and Dialysate: Towards Unravelling Renal Peptide Handling. Proteomics. Clinical Applications, e2000029. https://doi.org/10.1002/prca.202000029
He T, et al. Peptides in Blood, Urine and Dialysate: Towards Unravelling Renal Peptide Handling. Proteomics Clin Appl. 2020 Jul 3;e2000029. PubMed PMID: 32618437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peptides in Blood, Urine and Dialysate: Towards Unravelling Renal Peptide Handling. AU - He,Tianlin, AU - Pejchinovski,Martin, AU - Mullen,William, AU - Beige,Joachim, AU - Mischak,Harald, AU - Jankowski,Vera, Y1 - 2020/07/03/ PY - 2020/7/4/entrez KW - hemodialysis fluid KW - peptidomes KW - plasma KW - urine SP - e2000029 EP - e2000029 JF - Proteomics. Clinical applications JO - Proteomics Clin Appl N2 - PURPOSE: We investigated the peptidomes of spent hemodialysate, urine, and plasma, to shed light on peptide handling in the kidney. EXPERIMENTAL DESIGN: We collected 15 plasma, 15 urine and 13 spent hemodialysate samples from age and sex-matched subjects with chronic kidney disease. Peptide identification and quantification were performed with capillary electrophoresis-coupled mass spectrometry. RESULTS: We detected 6278 urinary peptides, 1743 plasma peptides and 1727 peptides from spent hemodialysate. Of these, we could assign sequences to 1580, 419 and 352 peptides respectively. We found a strong correlation in peptide abundance between urine and spent hemodialysate (P = 3e-21, Rho = 0.52), a moderately strong correlation between spent hemodialysate and plasma (P = 4.5e-5, Rho = 0.30), and no significant correlation between urine and plasma (P = 0.11, Rho = 0.094). Collagen and fibrinogen alpha peptides are highly abundant in all three body fluids. In spent hemodialysate, thymosin β4 is one of the most abundant peptides, which is shown to be negatively associated with the estimated glomerular filtration rate (Rho = -0.39, p-value = 3.9e-81). CONCLUSION AND CLINICAL RELEVANCE: The correlation of peptide abundance in these three body fluids is lower than expected, supporting the hypothesis that tubular reabsorption has a major impact on urinary peptide content. Further investigation of thymosin β4 in hemodialysis is thus warranted. This article is protected by copyright. All rights reserved. SN - 1862-8354 UR - https://www.unboundmedicine.com/medline/citation/32618437/Peptides_in_Blood,_Urine_and_Dialysate:_Towards_Unravelling_Renal_Peptide_Handling L2 - https://doi.org/10.1002/prca.202000029 DB - PRIME DP - Unbound Medicine ER -
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