Tags

Type your tag names separated by a space and hit enter

Acinetobacter portensis sp. nov. and Acinetobacter guerrae sp. nov., isolated from raw meat.
Int J Syst Evol Microbiol. 2020 Jul 03 [Online ahead of print]IJ

Abstract

The taxonomic status of six strains of Acinetobacter obtained from meat samples, collected from supermarkets in Porto, Portugal, was investigated using polyphasic analysis. Partial rpoB sequence similarities lower than 95 % to other Acinetobacter species with validly published names led to the hypothesis that these strains represented novel species. This was confirmed based on comparative multilocus sequence analysis, which included the gyrB, recA and 16S rRNA genes, revealing that these strains represented two coherent lineages that were distinct from each other and from all known species. The names Acinetobacter portensis sp. nov. (comprising four strains) and Acinetobacter guerrae sp. nov. (comprising two strains) are proposed for these novel species. The species status of these two groups was confirmed by low (below 95 %) whole-genome sequence average nucleotide identity values and low (below 70 %) digital DNA-DNA hybridization similarities between the whole-genome sequences of the proposed type strains of each novel species and the representatives of the known Acinetobacter species. Phylogenomic treeing from core genome analysis supported these results. The coherence of each new species lineage was supported by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry differentiation of the species at the protein level, by cellular fatty acid profiles, and by unique and differential combinations of metabolic and physiological properties shared by each novel species. The type strain of A. portensis sp. nov. is AC 877T (=CCUG 68672T=CCM 8789T) and the type strain of A. guerrae sp. nov. is AC 1271T (=CCUG 68674T=CCM 8791T).

Authors+Show Affiliations

Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.Centre for Antibiotic Resistance Research (CARe) at University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden. Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden.Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden. Microbiology, Department of Biology, University of the Balearic Islands, Palma de Mallorca, Spain. Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Centre for Antibiotic Resistance Research (CARe) at University of Gothenburg, Gothenburg, Sweden. Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden.Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden.Center for Translational Microbiome Research, Department of Microbiology, Cell and Tumor biology, Karolinska Institutet, Stockholm, Sweden. Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden.Centre for Antibiotic Resistance Research (CARe) at University of Gothenburg, Gothenburg, Sweden. Nanoxis Consulting AB, Gothenburg, Sweden. Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden.Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Centre for Antibiotic Resistance Research (CARe) at University of Gothenburg, Gothenburg, Sweden. Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32618559

Citation

Carvalheira, Ana, et al. "Acinetobacter Portensis Sp. Nov. and Acinetobacter Guerrae Sp. Nov., Isolated From Raw Meat." International Journal of Systematic and Evolutionary Microbiology, 2020.
Carvalheira A, Gonzales-Siles L, Salvà-Serra F, et al. Acinetobacter portensis sp. nov. and Acinetobacter guerrae sp. nov., isolated from raw meat. Int J Syst Evol Microbiol. 2020.
Carvalheira, A., Gonzales-Siles, L., Salvà-Serra, F., Lindgren, Å., Svensson-Stadler, L., Thorell, K., Piñeiro-Iglesias, B., Karlsson, R., Silva, J., Teixeira, P., & Moore, E. R. B. (2020). Acinetobacter portensis sp. nov. and Acinetobacter guerrae sp. nov., isolated from raw meat. International Journal of Systematic and Evolutionary Microbiology. https://doi.org/10.1099/ijsem.0.004311
Carvalheira A, et al. Acinetobacter Portensis Sp. Nov. and Acinetobacter Guerrae Sp. Nov., Isolated From Raw Meat. Int J Syst Evol Microbiol. 2020 Jul 3; PubMed PMID: 32618559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acinetobacter portensis sp. nov. and Acinetobacter guerrae sp. nov., isolated from raw meat. AU - Carvalheira,Ana, AU - Gonzales-Siles,Lucia, AU - Salvà-Serra,Francisco, AU - Lindgren,Åsa, AU - Svensson-Stadler,Liselott, AU - Thorell,Kaisa, AU - Piñeiro-Iglesias,Beatriz, AU - Karlsson,Roger, AU - Silva,Joana, AU - Teixeira,Paula, AU - Moore,Edward R B, Y1 - 2020/07/03/ PY - 2020/7/4/entrez KW - Acinetobacter KW - MLSA KW - core genome KW - novel species in meat KW - phylogenomics KW - whole-genome sequence JF - International journal of systematic and evolutionary microbiology JO - Int. J. Syst. Evol. Microbiol. N2 - The taxonomic status of six strains of Acinetobacter obtained from meat samples, collected from supermarkets in Porto, Portugal, was investigated using polyphasic analysis. Partial rpoB sequence similarities lower than 95 % to other Acinetobacter species with validly published names led to the hypothesis that these strains represented novel species. This was confirmed based on comparative multilocus sequence analysis, which included the gyrB, recA and 16S rRNA genes, revealing that these strains represented two coherent lineages that were distinct from each other and from all known species. The names Acinetobacter portensis sp. nov. (comprising four strains) and Acinetobacter guerrae sp. nov. (comprising two strains) are proposed for these novel species. The species status of these two groups was confirmed by low (below 95 %) whole-genome sequence average nucleotide identity values and low (below 70 %) digital DNA-DNA hybridization similarities between the whole-genome sequences of the proposed type strains of each novel species and the representatives of the known Acinetobacter species. Phylogenomic treeing from core genome analysis supported these results. The coherence of each new species lineage was supported by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry differentiation of the species at the protein level, by cellular fatty acid profiles, and by unique and differential combinations of metabolic and physiological properties shared by each novel species. The type strain of A. portensis sp. nov. is AC 877T (=CCUG 68672T=CCM 8789T) and the type strain of A. guerrae sp. nov. is AC 1271T (=CCUG 68674T=CCM 8791T). SN - 1466-5034 UR - https://www.unboundmedicine.com/medline/citation/32618559/Acinetobacter_portensis_sp._nov._and_Acinetobacter_guerrae_sp._nov.,_isolated_from_raw_meat L2 - http://ijs.microbiologyresearch.org/pubmed/content/journal/ijsem/10.1099/ijsem.0.004311 DB - PRIME DP - Unbound Medicine ER -