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Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models.
Urol J. 2020 06 23; 17(4):413-421.UJ

Abstract

PURPOSE

To evaluate the effect of a new mesenchymal stem cell type derived from the neonatal bladder (nMSC-B) on diabetic bladder dysfunction (DBD).

MATERIALS AND METHODS

nMSC-B were harvested from neonatal male Sprague-Dawley rat's bladder and expanded in culture. nMSC-B were transferred to Type-1 diabetic rats which were induced by a single dose 45 mg/kg Streptozocin (STZ). Stem cells were transferred via intraperitoneally (IP) (DM-IP group, n:6) and by direct injection to the detrusor (DM-D group, n:6) at 12th week following diabetes and compared with Phosphate Buffered Saline (PBS) injected diabetic rats (DM-PBS group, n:6) and age-matched PBS injected non-diabetic normal rats (NR-PBS group, n:6). All rats were evaluated histopathologically and functionally four weeks after the stem cell treatment.

RESULTS

nMSC-B showed improvement in both voiding function and bladder structure. The maximum voiding pressure (MVP) values in the DM-PBS group were lower compare to DM-IP, DM-D and NR-PBS groups (13.27 ± 0.78 vs 16.27 ± 0.61, 28.59 ± 2.09, 21.54 ± 1.00, respectively, P < .001). There was a significant improvement for MVP values in stem cell-treated groups. Immunohistochemical examination revealed decreased bladder smooth muscle (SM), increased fibrosis and desquamation in urothelia in diabetic groups compared to normal group(P < .001). We detected recovery in the stem cell groups. This recovery was more evident in DM-D group. No statistical difference was observed in SM and fibrosis between DM-D and NR-PBS groups (P = .9).

CONCLUSION

It was shown that nMSCBs provided amelioration of DBD. We think that nMSC-B constitutes an effective treatment method in DBD.

Authors+Show Affiliations

Department of Urology, Health Sciences University, Antalya Training and Research Hospital, Antalya, Turkey. msalihboga@yahoo.com.Department of Urology, Ufuk University, School of Medicine, Ankara, Turkey.Department of Urology, Ankara University, School of Medicine, Ankara, Turkey.Department of Urology, Health Sciences University, Atatürk Training and Research Hospital, Ankara, Turkey.Department of Urology, Necmettin Erbakan University, Meram School of Medicine, Konya, Turkey.Department of Genetics and Cell Research, Health Sciences University, Diskapi Yildirim Beyazit Training And Research Hospital, Ankara, Turkey.Independent Researcher, Copenhagen, Denmark.Department of Pathology, Health Sciences University, Antalya Training and Research Hospital, Antalya, Turkey.Department of Cell Research, Health Sciences University, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.Department of Urology, Ufuk University, School of Medicine, Ankara, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619015

Citation

Boga, Mehmet Salih, et al. "Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models." Urology Journal, vol. 17, no. 4, 2020, pp. 413-421.
Boga MS, Haliloğlu AH, Gülpınar Ö, et al. Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models. Urol J. 2020;17(4):413-421.
Boga, M. S., Haliloğlu, A. H., Gülpınar, Ö., Özayar, A., Sönmez, M. G., Pinarli, F. A., Boğa, E., Pinarci, T., Tiryaki, M., & Göğüş, O. (2020). Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models. Urology Journal, 17(4), 413-421. https://doi.org/10.22037/uj.v0i0.5504
Boga MS, et al. Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models. Urol J. 2020 06 23;17(4):413-421. PubMed PMID: 32619015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neonatal Bladder-derived Mesenchymal Stem Cells Ameliorate Bladder Dysfunction in Diabetic Rat Models. AU - Boga,Mehmet Salih, AU - Haliloğlu,Ahmet Hakan, AU - Gülpınar,Ömer, AU - Özayar,Asım, AU - Sönmez,Mehmet Giray, AU - Pinarli,Ferda Alpaslan, AU - Boğa,Emre, AU - Pinarci,Tangül, AU - Tiryaki,Meral, AU - Göğüş,Orhan, Y1 - 2020/06/23/ PY - 2019/08/06/received PY - 2020/01/15/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline SP - 413 EP - 421 JF - Urology journal JO - Urol J VL - 17 IS - 4 N2 - PURPOSE: To evaluate the effect of a new mesenchymal stem cell type derived from the neonatal bladder (nMSC-B) on diabetic bladder dysfunction (DBD). MATERIALS AND METHODS: nMSC-B were harvested from neonatal male Sprague-Dawley rat's bladder and expanded in culture. nMSC-B were transferred to Type-1 diabetic rats which were induced by a single dose 45 mg/kg Streptozocin (STZ). Stem cells were transferred via intraperitoneally (IP) (DM-IP group, n:6) and by direct injection to the detrusor (DM-D group, n:6) at 12th week following diabetes and compared with Phosphate Buffered Saline (PBS) injected diabetic rats (DM-PBS group, n:6) and age-matched PBS injected non-diabetic normal rats (NR-PBS group, n:6). All rats were evaluated histopathologically and functionally four weeks after the stem cell treatment. RESULTS: nMSC-B showed improvement in both voiding function and bladder structure. The maximum voiding pressure (MVP) values in the DM-PBS group were lower compare to DM-IP, DM-D and NR-PBS groups (13.27 ± 0.78 vs 16.27 ± 0.61, 28.59 ± 2.09, 21.54 ± 1.00, respectively, P < .001). There was a significant improvement for MVP values in stem cell-treated groups. Immunohistochemical examination revealed decreased bladder smooth muscle (SM), increased fibrosis and desquamation in urothelia in diabetic groups compared to normal group(P < .001). We detected recovery in the stem cell groups. This recovery was more evident in DM-D group. No statistical difference was observed in SM and fibrosis between DM-D and NR-PBS groups (P = .9). CONCLUSION: It was shown that nMSCBs provided amelioration of DBD. We think that nMSC-B constitutes an effective treatment method in DBD. SN - 1735-546X UR - https://www.unboundmedicine.com/medline/citation/32619015/Neonatal_Bladder-derived_Mesenchymal_Stem_Cells_Ameliorate_Bladder_Dysfunction_in_Diabetic_Rat_Models L2 - http://journals.sbmu.ac.ir/urolj/index.php/uj/article/view/5504/3936 DB - PRIME DP - Unbound Medicine ER -
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