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Protective effect of microRNA-134-3p on multiple sclerosis through inhibiting PRSS57 and promotion of CD34+ cell proliferation in rats.
J Cell Biochem. 2020 Jul 03 [Online ahead of print]JC

Abstract

MicroRNAs (miRs) have been extensively studied for their involvement in multiple sclerosis (MS). We investigated the involvement of miR-134-3p on MS. The MS rat model was established, and positive expression of interleukin-17 (IL-17) was detected using the immunohistochemical method while the expression of miR-134-3p and serine protease 57 (PRSS57) was determined by means of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis. Second, the miR-134-3p overexpression or short hairpin RNA against PRSS57 was introduced into the CD34+ cells to investigate the levels of proliferation and apoptosis-related genes by RT-qPCR and Western blot analysis. In addition, analysis of the targeting relations of miR-134-3p and PRSS57 was conducted using online software and dual-luciferase reporter gene assay. Furthermore, neuronal functions, inflammatory response, proliferation, and apoptosis of CD34+ cells were assayed by flow cytometry, enzyme-linked immunosorbent assay, and methyl thiazolyl tetrazolium. IL-17 and PRSS57 expression increased while miR-134-3p expression decreased in the spinal cord from MS rats. miR-134-3p could target PRSS57. miR-134-3p overexpression or PRSS57 silencing enhanced mitochondrial activity of neurons, mitochondrial membrane potential content, CD34+ cell proliferation, while decreasing Cyt C content, inflammatory response, and cell apoptosis. Collectively, overexpression of miR-134-3p promotes CD34+ cell proliferation via inhibition of PRSS57 in MS, which may serve as a promising target for MS intervention.

Authors+Show Affiliations

Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing, China. Department of Neurology, The No.2 Hospital of Baoding, Baoding, China.Department of Neurology, The No.2 Hospital of Baoding, Baoding, China.Department of Neurology, Beijing Tiantan Hospital of Capital Medical University, Beijing, China.Department of Neurology, The No.2 Hospital of Baoding, Baoding, China.Department of Neurology, The No.2 Hospital of Baoding, Baoding, China.Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619071

Citation

Song, Qihan, et al. "Protective Effect of microRNA-134-3p On Multiple Sclerosis Through Inhibiting PRSS57 and Promotion of CD34+ Cell Proliferation in Rats." Journal of Cellular Biochemistry, 2020.
Song Q, Zhao F, Yao J, et al. Protective effect of microRNA-134-3p on multiple sclerosis through inhibiting PRSS57 and promotion of CD34+ cell proliferation in rats. J Cell Biochem. 2020.
Song, Q., Zhao, F., Yao, J., Dai, H., Hu, L., & Yu, S. (2020). Protective effect of microRNA-134-3p on multiple sclerosis through inhibiting PRSS57 and promotion of CD34+ cell proliferation in rats. Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.29643
Song Q, et al. Protective Effect of microRNA-134-3p On Multiple Sclerosis Through Inhibiting PRSS57 and Promotion of CD34+ Cell Proliferation in Rats. J Cell Biochem. 2020 Jul 3; PubMed PMID: 32619071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of microRNA-134-3p on multiple sclerosis through inhibiting PRSS57 and promotion of CD34+ cell proliferation in rats. AU - Song,Qihan, AU - Zhao,Fengli, AU - Yao,Jingfan, AU - Dai,Hailin, AU - Hu,Lei, AU - Yu,Shun, Y1 - 2020/07/03/ PY - 2019/08/11/received PY - 2019/12/19/accepted PY - 2020/7/4/entrez KW - CD34+ cells KW - PRSS57 KW - apoptosis KW - microRNA-134-3p KW - multiple sclerosis KW - proliferation JF - Journal of cellular biochemistry JO - J. Cell. Biochem. N2 - MicroRNAs (miRs) have been extensively studied for their involvement in multiple sclerosis (MS). We investigated the involvement of miR-134-3p on MS. The MS rat model was established, and positive expression of interleukin-17 (IL-17) was detected using the immunohistochemical method while the expression of miR-134-3p and serine protease 57 (PRSS57) was determined by means of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis. Second, the miR-134-3p overexpression or short hairpin RNA against PRSS57 was introduced into the CD34+ cells to investigate the levels of proliferation and apoptosis-related genes by RT-qPCR and Western blot analysis. In addition, analysis of the targeting relations of miR-134-3p and PRSS57 was conducted using online software and dual-luciferase reporter gene assay. Furthermore, neuronal functions, inflammatory response, proliferation, and apoptosis of CD34+ cells were assayed by flow cytometry, enzyme-linked immunosorbent assay, and methyl thiazolyl tetrazolium. IL-17 and PRSS57 expression increased while miR-134-3p expression decreased in the spinal cord from MS rats. miR-134-3p could target PRSS57. miR-134-3p overexpression or PRSS57 silencing enhanced mitochondrial activity of neurons, mitochondrial membrane potential content, CD34+ cell proliferation, while decreasing Cyt C content, inflammatory response, and cell apoptosis. Collectively, overexpression of miR-134-3p promotes CD34+ cell proliferation via inhibition of PRSS57 in MS, which may serve as a promising target for MS intervention. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/32619071/Protective_effect_of_microRNA-134-3p_on_multiple_sclerosis_through_inhibiting_PRSS57_and_promotion_of_CD34+_cell_proliferation_in_rats L2 - https://doi.org/10.1002/jcb.29643 DB - PRIME DP - Unbound Medicine ER -
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