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Development and validation of an economical uric acid-Fe3+/Fe2+-ferrozine-based colorimetric assay to estimate uric acid level of pure and biological samples.
Biosci Biotechnol Biochem. 2020 Jul 03 [Online ahead of print]BB

Abstract

This work presents the development and validation of a simple, rapid, and cost-effective spectrophotometric method for quantitative analysis of uric acid in biological samples. The method relies upon uric acid-led reduction of Fe(III) to Fe(II) of sample/standard solutions which stoichiometrically engages ferrozine to form a magenta-colored complex. Different parameters including pH, metal and chelator concentrations, temperature, etc., were optimized for the maximum intensity and stability of the complex. The uric acid concentrations of synthetic/plasma solutions were determined by comparing the color intensity of Fe(ferrozine)3 2+ complex produced by test solution with the standard curve formed by known uric acid concentrations. The method was validated in accordance with ICH guidelines and subjected to human plasma analysis. The results obtained were compared with a reference (enzymatic) method which revealed that there was no significant difference between the two methods at 95% confidence level. The method is highly specific, precise, linear, accurate, and robust.

Authors+Show Affiliations

Department of Chemistry, Forman Christian College (A Chartered University) , Lahore, Pakistan.Department of Chemistry, Forman Christian College (A Chartered University) , Lahore, Pakistan.Department of Chemistry, Forman Christian College (A Chartered University) , Lahore, Pakistan.Department of Chemistry, School of Science and Technology, University of Management and Technology , Lahore, Pakistan.Department of Chemistry, Government College Women University , Sialkot, Pakistan.Department of Chemistry, COMSATS University Islamabad , Lahore Campus, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619142

Citation

Akram, Maryam, et al. "Development and Validation of an Economical Uric acid-Fe3+/Fe2+-ferrozine-based Colorimetric Assay to Estimate Uric Acid Level of Pure and Biological Samples." Bioscience, Biotechnology, and Biochemistry, 2020, pp. 1-8.
Akram M, Asghar MN, Saleem Khan M, et al. Development and validation of an economical uric acid-Fe3+/Fe2+-ferrozine-based colorimetric assay to estimate uric acid level of pure and biological samples. Biosci Biotechnol Biochem. 2020.
Akram, M., Asghar, M. N., Saleem Khan, M., Shahid, S., Abdur Rahman, H. M., & Nadeem, I. (2020). Development and validation of an economical uric acid-Fe3+/Fe2+-ferrozine-based colorimetric assay to estimate uric acid level of pure and biological samples. Bioscience, Biotechnology, and Biochemistry, 1-8. https://doi.org/10.1080/09168451.2020.1781593
Akram M, et al. Development and Validation of an Economical Uric acid-Fe3+/Fe2+-ferrozine-based Colorimetric Assay to Estimate Uric Acid Level of Pure and Biological Samples. Biosci Biotechnol Biochem. 2020 Jul 3;1-8. PubMed PMID: 32619142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of an economical uric acid-Fe3+/Fe2+-ferrozine-based colorimetric assay to estimate uric acid level of pure and biological samples. AU - Akram,Maryam, AU - Asghar,Muhammad Nadeem, AU - Saleem Khan,Maria, AU - Shahid,Sammia, AU - Abdur Rahman,Hafiz Muhammad, AU - Nadeem,Iram, Y1 - 2020/07/03/ PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - ICH guidelines KW - Uric Acid KW - ferrozine SP - 1 EP - 8 JF - Bioscience, biotechnology, and biochemistry JO - Biosci. Biotechnol. Biochem. N2 - This work presents the development and validation of a simple, rapid, and cost-effective spectrophotometric method for quantitative analysis of uric acid in biological samples. The method relies upon uric acid-led reduction of Fe(III) to Fe(II) of sample/standard solutions which stoichiometrically engages ferrozine to form a magenta-colored complex. Different parameters including pH, metal and chelator concentrations, temperature, etc., were optimized for the maximum intensity and stability of the complex. The uric acid concentrations of synthetic/plasma solutions were determined by comparing the color intensity of Fe(ferrozine)3 2+ complex produced by test solution with the standard curve formed by known uric acid concentrations. The method was validated in accordance with ICH guidelines and subjected to human plasma analysis. The results obtained were compared with a reference (enzymatic) method which revealed that there was no significant difference between the two methods at 95% confidence level. The method is highly specific, precise, linear, accurate, and robust. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/32619142/Development_and_validation_of_an_economical_uric_acid-Fe3+/Fe2+-ferrozine-based_colorimetric_assay_to_estimate_uric_acid_level_of_pure_and_biological_samples L2 - http://www.tandfonline.com/doi/full/10.1080/09168451.2020.1781593 DB - PRIME DP - Unbound Medicine ER -
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