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Low prevalence of hypervirulent Klebsiella pneumoniae in Anatolia, screened via phenotypic and genotypic testing.
Acta Microbiol Immunol Hung. 2020 Jul 02 [Online ahead of print]AM

Abstract

Hypervirulent Klebsiella pneumoniae (hvKP) strains are associated with vigorous clinical presentation and relapses. Initially reported from Asia, these variants have spread globally and become an emerging agent of significant health threat. This study was carried out to identify hvKP strains in a previously uninvestigated region and to evaluate the impact of commonly-employed phenotypic and genotypic markers as diagnostic assays. A total of 111 blood culture isolates, collected at a tertiary care center was investigated. The hvKP strains were sought by a string test and the amplification of partial magA, rmpA, iucA and peg344. All products were characterized via sequencing. Evidence for hvKP was observed in 10.8% via iucA amplification (7.2%), string test (2.7%) and magA amplification (0.9%). Specific products were not produced by assays targeting rmpA and peg344 genes. Antibiotic susceptibility patterns compatible with possible extensive or pan-antimicrobial resistance was noted in 66.7% of the hvKP candidate strains. Capsule type in the magA positive strain was characterized as K5. We have detected hvKP in low prevalence at a region with no prior documentation. Targetting the aerobactin gene via iucA amplification provided the most accurate detection in this setting. The epidemiology of hvKP in Anatolia requires elucidation for effective control and management.

Authors+Show Affiliations

1Vocational School of Health Services, Hacettepe University, Ankara, Turkey.2Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.2Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.2Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.2Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.2Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.2Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619189

Citation

Örsten, Serra, et al. "Low Prevalence of Hypervirulent Klebsiella Pneumoniae in Anatolia, Screened Via Phenotypic and Genotypic Testing." Acta Microbiologica Et Immunologica Hungarica, 2020.
Örsten S, Demirci-Duarte S, Ünalan-Altıntop T, et al. Low prevalence of hypervirulent Klebsiella pneumoniae in Anatolia, screened via phenotypic and genotypic testing. Acta Microbiol Immunol Hung. 2020.
Örsten, S., Demirci-Duarte, S., Ünalan-Altıntop, T., Çakar, A., Sancak, B., ErgÜnay, K., & Özkuyumcu, C. (2020). Low prevalence of hypervirulent Klebsiella pneumoniae in Anatolia, screened via phenotypic and genotypic testing. Acta Microbiologica Et Immunologica Hungarica. https://doi.org/10.1556/030.2020.01143
Örsten S, et al. Low Prevalence of Hypervirulent Klebsiella Pneumoniae in Anatolia, Screened Via Phenotypic and Genotypic Testing. Acta Microbiol Immunol Hung. 2020 Jul 2; PubMed PMID: 32619189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low prevalence of hypervirulent Klebsiella pneumoniae in Anatolia, screened via phenotypic and genotypic testing. AU - Örsten,Serra, AU - Demirci-Duarte,Selay, AU - Ünalan-Altıntop,TuĞçe, AU - Çakar,Aslı, AU - Sancak,Banu, AU - ErgÜnay,Koray, AU - Özkuyumcu,Cumhur, Y1 - 2020/07/02/ PY - 2020/01/19/received PY - 2020/02/03/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - Klebsiella pneumoniae KW - capsule KW - hypermucoviscous KW - hypervirulent JF - Acta microbiologica et immunologica Hungarica JO - Acta Microbiol Immunol Hung N2 - Hypervirulent Klebsiella pneumoniae (hvKP) strains are associated with vigorous clinical presentation and relapses. Initially reported from Asia, these variants have spread globally and become an emerging agent of significant health threat. This study was carried out to identify hvKP strains in a previously uninvestigated region and to evaluate the impact of commonly-employed phenotypic and genotypic markers as diagnostic assays. A total of 111 blood culture isolates, collected at a tertiary care center was investigated. The hvKP strains were sought by a string test and the amplification of partial magA, rmpA, iucA and peg344. All products were characterized via sequencing. Evidence for hvKP was observed in 10.8% via iucA amplification (7.2%), string test (2.7%) and magA amplification (0.9%). Specific products were not produced by assays targeting rmpA and peg344 genes. Antibiotic susceptibility patterns compatible with possible extensive or pan-antimicrobial resistance was noted in 66.7% of the hvKP candidate strains. Capsule type in the magA positive strain was characterized as K5. We have detected hvKP in low prevalence at a region with no prior documentation. Targetting the aerobactin gene via iucA amplification provided the most accurate detection in this setting. The epidemiology of hvKP in Anatolia requires elucidation for effective control and management. SN - 1588-2640 UR - https://www.unboundmedicine.com/medline/citation/32619189/Low_prevalence_of_hypervirulent_Klebsiella_pneumoniae_in_Anatolia,_screened_via_phenotypic_and_genotypic_testing L2 - https://akjournals.com/doi/10.1556/030.2020.01143 DB - PRIME DP - Unbound Medicine ER -
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