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Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273.
J Appl Microbiol. 2020 Jul 03 [Online ahead of print]JA

Abstract

AIMS

Relative few anti-biofilm polysaccharides against Pseudomonas aeruginosa were done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection of P. aeruginosa. This study aims to investigate its anti-biofilm and anti-infection mechanism on transcriptional level.

METHODS AND RESULTS

Herein we used an RNA-Seq transcriptomic approach to investigate the underlying anti-biofilm and anti-infection mechanism of EPS273. The expression levels of a large number of genes were changed after P. aeruginosa PAO1 was treated with EPS273. Especially, the genes related to biofilm formation, such as gene involved in production of extracellular matrix and virulence factor, genes involved in flagella and cell motility and genes involved in iron acquisition. Notably, the expression levels of genes involved in regulatory and signal transduction were markedly downregulated, such as two-component system PhoP-PhoQ and quorum sensing (QS) system LasI/ LasR and RhlI/ RhlR. Furthermore, when gene phoP and phoQ was disrupted respectively, the reduction of biofilm formation and cell motility in mutant △phoP or △phoQ was also detected.

CONCLUSION

EPS273 may exert its anti-biofilm and anti-infection function by downregulating gene expression of two-component system PhoP-PhoQ and QS systems LasI/ LasR and RhlI/ RhlR of P. aeruginosa, which further regulated expression of genes involved in biofilm formation.

SIGNIFICANCE AND IMPACT OF THE STUDY

Our data will expand understanding of anti-biofilm mechanisms of polysaccharides on transcriptomic level.

Authors+Show Affiliations

College of Life Sciences, Qingdao University, Qingdao, Shandong, China.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, China.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, China.College of Life Sciences, Qingdao University, Qingdao, Shandong, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619289

Citation

Wu, Zuodong, et al. "Transcriptional Profiling of Pseudomonas Aeruginosa PAO1 in Response to Anti-biofilm and Anti-infection Agent Exopolysaccharide EPS273." Journal of Applied Microbiology, 2020.
Wu Z, Zheng R, Zhang J, et al. Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273. J Appl Microbiol. 2020.
Wu, Z., Zheng, R., Zhang, J., & Wu, S. (2020). Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273. Journal of Applied Microbiology. https://doi.org/10.1111/jam.14764
Wu Z, et al. Transcriptional Profiling of Pseudomonas Aeruginosa PAO1 in Response to Anti-biofilm and Anti-infection Agent Exopolysaccharide EPS273. J Appl Microbiol. 2020 Jul 3; PubMed PMID: 32619289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273. AU - Wu,Zuodong, AU - Zheng,Rikuan, AU - Zhang,Jing, AU - Wu,Shimei, Y1 - 2020/07/03/ PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - Pseudomonas aeruginosa KW - biofilm KW - polysaccharide KW - transcriptomic KW - virulence factor JF - Journal of applied microbiology JO - J. Appl. Microbiol. N2 - AIMS: Relative few anti-biofilm polysaccharides against Pseudomonas aeruginosa were done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection of P. aeruginosa. This study aims to investigate its anti-biofilm and anti-infection mechanism on transcriptional level. METHODS AND RESULTS: Herein we used an RNA-Seq transcriptomic approach to investigate the underlying anti-biofilm and anti-infection mechanism of EPS273. The expression levels of a large number of genes were changed after P. aeruginosa PAO1 was treated with EPS273. Especially, the genes related to biofilm formation, such as gene involved in production of extracellular matrix and virulence factor, genes involved in flagella and cell motility and genes involved in iron acquisition. Notably, the expression levels of genes involved in regulatory and signal transduction were markedly downregulated, such as two-component system PhoP-PhoQ and quorum sensing (QS) system LasI/ LasR and RhlI/ RhlR. Furthermore, when gene phoP and phoQ was disrupted respectively, the reduction of biofilm formation and cell motility in mutant △phoP or △phoQ was also detected. CONCLUSION: EPS273 may exert its anti-biofilm and anti-infection function by downregulating gene expression of two-component system PhoP-PhoQ and QS systems LasI/ LasR and RhlI/ RhlR of P. aeruginosa, which further regulated expression of genes involved in biofilm formation. SIGNIFICANCE AND IMPACT OF THE STUDY: Our data will expand understanding of anti-biofilm mechanisms of polysaccharides on transcriptomic level. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/32619289/Transcriptional_profiling_of_Pseudomonas_aeruginosa_PAO1_in_response_to_anti-biofilm_and_anti-infection_agent_exopolysaccharide_EPS273 L2 - https://doi.org/10.1111/jam.14764 DB - PRIME DP - Unbound Medicine ER -
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