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Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China.
Mol Ecol Resour. 2020 Jul 03 [Online ahead of print]ME

Abstract

The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38Mb chromosome-level genome of fall armyworm derived from south-central Africa using Pacific Bioscience (PacBio) and Hi-C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bacillus thuringiensis (Bt) toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

Authors+Show Affiliations

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK.Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK.Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi.School of Forensic and Applied Sciences, University of Central Lancashire, Preston, PR1 2HE, UK.Zambia Agriculture Research Institute (ZARI), Lusaka, Zambia.Department of Agricultural Research Services (DARS), Bvumbwe Research Station, P. O. Box 5748, Limbe, Malawi.DGIMI, Univ. Montpellier, INRA, Montpellier, 34095, France.DGIMI, Univ. Montpellier, INRA, Montpellier, 34095, France.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.National Agro-Tech, Extension and Service Center, Beijing, 100125, China.National Agro-Tech, Extension and Service Center, Beijing, 100125, China.Novogene Bioinformatics Institute, Beijing, 100083, China.Grandomics Biosciences, Co., Ltd, Beijing, 102206, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619331

Citation

Zhang, Lei, et al. "Genetic Structure and Insecticide Resistance Characteristics of Fall Armyworm Populations Invading China." Molecular Ecology Resources, 2020.
Zhang L, Liu B, Zheng W, et al. Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Mol Ecol Resour. 2020.
Zhang, L., Liu, B., Zheng, W., Liu, C., Zhang, D., Zhao, S., Li, Z., Xu, P., Wilson, K., Withers, A., Jones, C. M., Smith, J. A., Chipabika, G., Kachigamba, D. L., Nam, K., d'Alençon, E., Liu, B., Liang, X., Jin, M., ... Xiao, Y. (2020). Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources. https://doi.org/10.1111/1755-0998.13219
Zhang L, et al. Genetic Structure and Insecticide Resistance Characteristics of Fall Armyworm Populations Invading China. Mol Ecol Resour. 2020 Jul 3; PubMed PMID: 32619331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. AU - Zhang,Lei, AU - Liu,Bo, AU - Zheng,Weigang, AU - Liu,Conghui, AU - Zhang,Dandan, AU - Zhao,Shengyuan, AU - Li,Zaiyuan, AU - Xu,Pengjun, AU - Wilson,Kenneth, AU - Withers,Amy, AU - Jones,Christopher M, AU - Smith,Judith A, AU - Chipabika,Gilson, AU - Kachigamba,Donald L, AU - Nam,Kiwoong, AU - d'Alençon,Emmanuelle, AU - Liu,Bei, AU - Liang,Xinyue, AU - Jin,Minghui, AU - Wu,Chao, AU - Chakrabarty,Swapan, AU - Yang,Xianming, AU - Jiang,Yuying, AU - Liu,Jie, AU - Liu,Xiaolin, AU - Quan,Weipeng, AU - Wang,Guirong, AU - Fan,Wei, AU - Qian,Wanqiang, AU - Wu,Kongming, AU - Xiao,Yutao, Y1 - 2020/07/03/ PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - Spodoptera frugiperda KW - gene insertion KW - population structure KW - resistance risk KW - subpopulations JF - Molecular ecology resources JO - Mol Ecol Resour N2 - The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38Mb chromosome-level genome of fall armyworm derived from south-central Africa using Pacific Bioscience (PacBio) and Hi-C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bacillus thuringiensis (Bt) toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm. SN - 1755-0998 UR - https://www.unboundmedicine.com/medline/citation/32619331/Genetic_structure_and_insecticide_resistance_characteristics_of_fall_armyworm_populations_invading_China L2 - https://doi.org/10.1111/1755-0998.13219 DB - PRIME DP - Unbound Medicine ER -
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