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Proposal for a microcosm biofilm model for the study of vulvovaginal candidiasis.
Biofouling. 2020 May; 36(5):610-620.B

Abstract

This study proposes a microcosm biofilm (MiB) model for the study of vulvovaginal candidiasis (VVC). Different conditions that mimic the vaginal environment were tested for MiB formation. The best growth conditions were obtained with samples incubated in vaginal fluid simulator medium pH 4.5 at 35 °C under a microaerophilic atmosphere. MiBs were evaluated for growth kinetics, fluconazole susceptibility and morphology. Samples containing high numbers of bacteria were analyzed for metagenomics. At 48 h, MiBs presented a higher cell density (CFU ml-1), a higher biomass and tolerance to fluconazole than their corresponding monospecies biofilms. Morphological analysis of MiBs revealed blastoconidia preferentially adhered to epithelial cells. Abundant Lactobacillus spp. were detected in two clinical samples; their MiBs showed a lower biomass and a higher fluconazole susceptibility. The proposed model proved to be a useful tool for the study of the complex microbial relationship in the vaginal environment, and may help to find new strategies for VVC control.

Authors+Show Affiliations

Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Instituto de Prevenção do Câncer, Fortaleza, Ceará, Brazil.Unichristus Centro Universitário Christus, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Tropical Medicine Department, Federal University of Pernambuco, Recife, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil.Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619353

Citation

Cordeiro, Rossana de Aguiar, et al. "Proposal for a Microcosm Biofilm Model for the Study of Vulvovaginal Candidiasis." Biofouling, vol. 36, no. 5, 2020, pp. 610-620.
Cordeiro RA, de Andrade ARC, Portela FVM, et al. Proposal for a microcosm biofilm model for the study of vulvovaginal candidiasis. Biofouling. 2020;36(5):610-620.
Cordeiro, R. A., de Andrade, A. R. C., Portela, F. V. M., Pereira, L. M. G., Moura, S. G. B., Sampaio, M. D., Pereira, E. M. A., de Melo Guedes, G. M., Bandeira, S. P., de Lima-Neto, R. G., Melo, V. M. M., Brilhante, R. S. N., Castelo-Branco, D. S. C. M., Rocha, M. F. G., & Sidrim, J. J. C. (2020). Proposal for a microcosm biofilm model for the study of vulvovaginal candidiasis. Biofouling, 36(5), 610-620. https://doi.org/10.1080/08927014.2020.1785435
Cordeiro RA, et al. Proposal for a Microcosm Biofilm Model for the Study of Vulvovaginal Candidiasis. Biofouling. 2020;36(5):610-620. PubMed PMID: 32619353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proposal for a microcosm biofilm model for the study of vulvovaginal candidiasis. AU - Cordeiro,Rossana de Aguiar, AU - de Andrade,Ana Raquel Colares, AU - Portela,Fernando Victor Monteiro, AU - Pereira,Lívia Maria Galdino, AU - Moura,Santiago Gonçalves Bezerra, AU - Sampaio,Mônica Dantas, AU - Pereira,Estefania Mota Araripe, AU - de Melo Guedes,Gláucia Morgana, AU - Bandeira,Silviane Praciano, AU - de Lima-Neto,Reginaldo Gonçalves, AU - Melo,Vânia Maria Maciel, AU - Brilhante,Raimunda Sâmia Nogueira, AU - Castelo-Branco,Débora Souza Collares Maia, AU - Rocha,Marcos Fábio Gadelha, AU - Sidrim,José Júlio Costa, Y1 - 2020/07/03/ PY - 2020/7/4/pubmed PY - 2020/7/4/medline PY - 2020/7/4/entrez KW - Candida KW - antifungal susceptibility KW - multiespecies biofilm KW - vaginitis SP - 610 EP - 620 JF - Biofouling JO - Biofouling VL - 36 IS - 5 N2 - This study proposes a microcosm biofilm (MiB) model for the study of vulvovaginal candidiasis (VVC). Different conditions that mimic the vaginal environment were tested for MiB formation. The best growth conditions were obtained with samples incubated in vaginal fluid simulator medium pH 4.5 at 35 °C under a microaerophilic atmosphere. MiBs were evaluated for growth kinetics, fluconazole susceptibility and morphology. Samples containing high numbers of bacteria were analyzed for metagenomics. At 48 h, MiBs presented a higher cell density (CFU ml-1), a higher biomass and tolerance to fluconazole than their corresponding monospecies biofilms. Morphological analysis of MiBs revealed blastoconidia preferentially adhered to epithelial cells. Abundant Lactobacillus spp. were detected in two clinical samples; their MiBs showed a lower biomass and a higher fluconazole susceptibility. The proposed model proved to be a useful tool for the study of the complex microbial relationship in the vaginal environment, and may help to find new strategies for VVC control. SN - 1029-2454 UR - https://www.unboundmedicine.com/medline/citation/32619353/Proposal_for_a_microcosm_biofilm_model_for_the_study_of_vulvovaginal_candidiasis L2 - http://www.tandfonline.com/doi/full/10.1080/08927014.2020.1785435 DB - PRIME DP - Unbound Medicine ER -
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