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Modeling the cornea in 3-dimensions: Current and future perspectives.
Exp Eye Res. 2020 Jun 30 [Online ahead of print]EE

Abstract

The cornea is an avascular, transparent ocular tissue that serves as a refractive and protective structure for the eye. Over 90% of the cornea is composed of a collagenous-rich extracellular matrix within the stroma with the other 10% composed by the corneal epithelium and endothelium layers and their corresponding supporting collagen layers (e.g., Bowman's and Descemet's membranes) at the anterior and posterior cornea, respectively. Due to its prominent role in corneal structure, tissue engineering approaches to model the human cornea in vitro have focused heavily on the cellular and functional properties of the corneal stroma. In this review, we discuss model development in the context of culture dimensionality (e.g., 2-dimensional versus 3-dimensional) and expand on the optical, biomechanical, and cellular functions promoted by the culture microenvironment. We describe current methods to model the human cornea with focus on organotypic approaches, compressed collagen, bioprinting, and self-assembled stromal models. We also expand on co-culture applications with the inclusion of relevant corneal cell types, such as epithelial, stromal keratocyte or fibroblast, endothelial, and neuronal cells. Further advancements in corneal tissue model development will markedly improve our current understanding of corneal wound healing and regeneration.

Authors+Show Affiliations

Schepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, 02114, USA.Schepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, 02114, USA.Schepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, 02114, USA.Schepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, 02114, USA.North Texas Eye Research Institute, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA; Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA. Electronic address: Dimitrios.Karamichos@unthsc.edu.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

32619578

Citation

McKay, Tina B., et al. "Modeling the Cornea in 3-dimensions: Current and Future Perspectives." Experimental Eye Research, 2020, p. 108127.
McKay TB, Hutcheon AEK, Guo X, et al. Modeling the cornea in 3-dimensions: Current and future perspectives. Exp Eye Res. 2020.
McKay, T. B., Hutcheon, A. E. K., Guo, X., Zieske, J. D., & Karamichos, D. (2020). Modeling the cornea in 3-dimensions: Current and future perspectives. Experimental Eye Research, 108127. https://doi.org/10.1016/j.exer.2020.108127
McKay TB, et al. Modeling the Cornea in 3-dimensions: Current and Future Perspectives. Exp Eye Res. 2020 Jun 30;108127. PubMed PMID: 32619578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modeling the cornea in 3-dimensions: Current and future perspectives. AU - McKay,Tina B, AU - Hutcheon,Audrey E K, AU - Guo,Xiaoqing, AU - Zieske,James D, AU - Karamichos,Dimitrios, Y1 - 2020/06/30/ PY - 2020/03/22/received PY - 2020/06/17/revised PY - 2020/06/23/accepted PY - 2020/7/4/entrez PY - 2020/7/4/pubmed PY - 2020/7/4/medline KW - Collagen KW - Cornea KW - Extracellular matrix KW - Stroma KW - Tissue-equivalents KW - Vitamin C SP - 108127 EP - 108127 JF - Experimental eye research JO - Exp. Eye Res. N2 - The cornea is an avascular, transparent ocular tissue that serves as a refractive and protective structure for the eye. Over 90% of the cornea is composed of a collagenous-rich extracellular matrix within the stroma with the other 10% composed by the corneal epithelium and endothelium layers and their corresponding supporting collagen layers (e.g., Bowman's and Descemet's membranes) at the anterior and posterior cornea, respectively. Due to its prominent role in corneal structure, tissue engineering approaches to model the human cornea in vitro have focused heavily on the cellular and functional properties of the corneal stroma. In this review, we discuss model development in the context of culture dimensionality (e.g., 2-dimensional versus 3-dimensional) and expand on the optical, biomechanical, and cellular functions promoted by the culture microenvironment. We describe current methods to model the human cornea with focus on organotypic approaches, compressed collagen, bioprinting, and self-assembled stromal models. We also expand on co-culture applications with the inclusion of relevant corneal cell types, such as epithelial, stromal keratocyte or fibroblast, endothelial, and neuronal cells. Further advancements in corneal tissue model development will markedly improve our current understanding of corneal wound healing and regeneration. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/32619578/Modeling_the_cornea_in_3-dimensions:_Current_and_future_perspectives L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(20)30385-7 DB - PRIME DP - Unbound Medicine ER -
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