Tags

Type your tag names separated by a space and hit enter

SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy.
Sci Total Environ. 2020 Nov 10; 742:140540.ST

Abstract

The COVID-19 outbreak has rapidly progressed worldwide finding the health system, scientists and society unprepared to face a little-known, fast spreading, and extremely deadly virus. Italy is one of the countries hardest hit by the pandemic, resulting in healthcare facilities bearing heavy burdens and severe restrictive measures. Despite efforts to clarify the virus transmission, especially in indoor scenarios, several aspects of SARS-CoV-2 spread are still rudimentary. This study evaluated the contamination of the air and surfaces by SARS-CoV-2 RNA in the COVID-19 isolation ward of a hospital in Milan, Italy. A total of 42 air and surface samples were collected inside five different zones of the ward including contaminated (COVID-19 patients' area), semi-contaminated (undressing room), and clean areas. SARS-CoV-2 RNA detection was performed using real time reverse transcription polymerase chain reaction. Overall, 24.3% of swab samples were positive, but none of these were collected in the clean area. Thus, the positivity rate was higher in contaminated (35.0%) and semi-contaminated (50.0%) areas than in clean areas (0.0%; P<0.05). The most contaminated surfaces were hand sanitizer dispensers (100.0%), medical equipment (50.0%), medical equipment touch screens (50.0%), shelves for medical equipment (40.0%), bedrails (33.3%), and door handles (25.0%). All the air samples collected from the contaminated area, namely the intensive care unit and corridor, were positive while viral RNA was not detected in either semi-contaminated or clean areas. These results showed that environmental contamination did not involve clean areas, but the results also support the need for strict disinfection, hand hygiene and protective measures for healthcare workers as well as the need for airborne isolation precautions.

Authors+Show Affiliations

Saints Paolo and Carlo Hospital, Via Antonio di Rudinì, 8, 20142 Milan, Italy.Department of Health, Animal Science and Food Safety "Carlo Cantoni", University of Milan, Via Celoria, 10, 20133 Milan, Italy. Electronic address: marta.castrica@unimi.it.Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy. Electronic address: laura.menchetti7@gmail.com.Chemservice S.r.l. - Lab Analysis Group, Via F. lli Beltrami, 15, 20026 Novate Milanese, Milan, Italy.Saints Paolo and Carlo Hospital, Via Antonio di Rudinì, 8, 20142 Milan, Italy.Saints Paolo and Carlo Hospital, Via Antonio di Rudinì, 8, 20142 Milan, Italy.Saints Paolo and Carlo Hospital, Via Antonio di Rudinì, 8, 20142 Milan, Italy.Saints Paolo and Carlo Hospital, Via Antonio di Rudinì, 8, 20142 Milan, Italy.Saints Paolo and Carlo Hospital, Via Antonio di Rudinì, 8, 20142 Milan, Italy.Department of Health, Animal Science and Food Safety "Carlo Cantoni", University of Milan, Via Celoria, 10, 20133 Milan, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619843

Citation

Razzini, Katia, et al. "SARS-CoV-2 RNA Detection in the Air and On Surfaces in the COVID-19 Ward of a Hospital in Milan, Italy." The Science of the Total Environment, vol. 742, 2020, p. 140540.
Razzini K, Castrica M, Menchetti L, et al. SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy. Sci Total Environ. 2020;742:140540.
Razzini, K., Castrica, M., Menchetti, L., Maggi, L., Negroni, L., Orfeo, N. V., Pizzoccheri, A., Stocco, M., Muttini, S., & Balzaretti, C. M. (2020). SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy. The Science of the Total Environment, 742, 140540. https://doi.org/10.1016/j.scitotenv.2020.140540
Razzini K, et al. SARS-CoV-2 RNA Detection in the Air and On Surfaces in the COVID-19 Ward of a Hospital in Milan, Italy. Sci Total Environ. 2020 Nov 10;742:140540. PubMed PMID: 32619843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy. AU - Razzini,Katia, AU - Castrica,Marta, AU - Menchetti,Laura, AU - Maggi,Lorenzo, AU - Negroni,Lucia, AU - Orfeo,Nicola V, AU - Pizzoccheri,Alice, AU - Stocco,Matteo, AU - Muttini,Stefano, AU - Balzaretti,Claudia M, Y1 - 2020/06/26/ PY - 2020/05/27/received PY - 2020/06/24/revised PY - 2020/06/24/accepted PY - 2020/7/4/pubmed PY - 2020/9/12/medline PY - 2020/7/4/entrez KW - Airborne transmission KW - COVID-19 KW - Coronavirus KW - Environmental contamination KW - Infection control KW - Outbreak SP - 140540 EP - 140540 JF - The Science of the total environment JO - Sci Total Environ VL - 742 N2 - The COVID-19 outbreak has rapidly progressed worldwide finding the health system, scientists and society unprepared to face a little-known, fast spreading, and extremely deadly virus. Italy is one of the countries hardest hit by the pandemic, resulting in healthcare facilities bearing heavy burdens and severe restrictive measures. Despite efforts to clarify the virus transmission, especially in indoor scenarios, several aspects of SARS-CoV-2 spread are still rudimentary. This study evaluated the contamination of the air and surfaces by SARS-CoV-2 RNA in the COVID-19 isolation ward of a hospital in Milan, Italy. A total of 42 air and surface samples were collected inside five different zones of the ward including contaminated (COVID-19 patients' area), semi-contaminated (undressing room), and clean areas. SARS-CoV-2 RNA detection was performed using real time reverse transcription polymerase chain reaction. Overall, 24.3% of swab samples were positive, but none of these were collected in the clean area. Thus, the positivity rate was higher in contaminated (35.0%) and semi-contaminated (50.0%) areas than in clean areas (0.0%; P<0.05). The most contaminated surfaces were hand sanitizer dispensers (100.0%), medical equipment (50.0%), medical equipment touch screens (50.0%), shelves for medical equipment (40.0%), bedrails (33.3%), and door handles (25.0%). All the air samples collected from the contaminated area, namely the intensive care unit and corridor, were positive while viral RNA was not detected in either semi-contaminated or clean areas. These results showed that environmental contamination did not involve clean areas, but the results also support the need for strict disinfection, hand hygiene and protective measures for healthcare workers as well as the need for airborne isolation precautions. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/32619843/SARS_CoV_2_RNA_detection_in_the_air_and_on_surfaces_in_the_COVID_19_ward_of_a_hospital_in_Milan_Italy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(20)34062-6 DB - PRIME DP - Unbound Medicine ER -