Tags

Type your tag names separated by a space and hit enter

Analysis of adjunctive serological detection to nucleic acid test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection diagnosis.
Int Immunopharmacol. 2020 Sep; 86:106746.II

Abstract

BACKGROUND

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused coronavirus disease 2019 (COVID-19) epidemic in China, December 2019. The clinical features and treatment of COVID-19 patients remain largely elusive. However, accurate detection is required for SARS-CoV-2 infection diagnosis. We aimed to evaluate the antibodies-based test and nucleic acid-based test for SARS-CoV-2-infected patients.

METHODS

We retrospectively studied 133 patients diagnosed with SARS-CoV-2 and admitted to Renmin Hospital of Wuhan University, China, from January 23 to March 1, 2020. Demographic data, clinical records, laboratory tests, and outcomes were collected. Data were accessed by SARS-CoV-2 IgM-IgG antibody test and real-time reverse transcriptase PCR (RT-PCR) detection for SARS-CoV-2 nucleic acid in COVID-19 patients.

RESULTS

Of 133 COVID-19 patients, there were 44 moderate cases, 52 severe cases, and 37 critical cases with no differences in gender and age among three subgroups. In RT-PCR detection, the positive rate was 65.9%, 71.2%, and 67.6% in moderate, severe, and critical cases, respectively. Whereas the positive rate of IgM/IgG antibody detection in patients was 79.5%/93.2%, 82.7%/100%, and 73.0%/97.3% in moderate, severe, and critical cases, respectively. Moreover, the IgM and IgG antibodies concentrations were also examined with no differences among three subgroups.

CONCLUSION

The IgM-IgG antibody test exhibited a useful adjunct to RT-PCR detection, and improved the accuracy in COVID-19 diagnosis regardless of the severity of illness, which provides an effective complement to the false-negative results from a nucleic acid test for SARS-CoV-2 infection diagnosis after onsets.

Authors+Show Affiliations

Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.Department of Clinical Laboratory, Shanghai Gongli Hospital, the Second Military Medical University, Pudong New Area, Shanghai 200135, PR China.Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China.State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Wuhan Institute of Biotechnology, Wuhan 430075, PR China.State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, PR China. Electronic address: zhluo18@jnu.edu.cn.Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, PR China. Electronic address: xinchengzhu@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32619956

Citation

Liu, Rui, et al. "Analysis of Adjunctive Serological Detection to Nucleic Acid Test for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection Diagnosis." International Immunopharmacology, vol. 86, 2020, p. 106746.
Liu R, Liu X, Yuan L, et al. Analysis of adjunctive serological detection to nucleic acid test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection diagnosis. Int Immunopharmacol. 2020;86:106746.
Liu, R., Liu, X., Yuan, L., Han, H., Shereen, M. A., Zhen, J., Niu, Z., Li, D., Liu, F., Wu, K., Luo, Z., & Zhu, C. (2020). Analysis of adjunctive serological detection to nucleic acid test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection diagnosis. International Immunopharmacology, 86, 106746. https://doi.org/10.1016/j.intimp.2020.106746
Liu R, et al. Analysis of Adjunctive Serological Detection to Nucleic Acid Test for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection Diagnosis. Int Immunopharmacol. 2020;86:106746. PubMed PMID: 32619956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of adjunctive serological detection to nucleic acid test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection diagnosis. AU - Liu,Rui, AU - Liu,Xinghui, AU - Yuan,Li, AU - Han,Huan, AU - Shereen,Muhammad Adnan, AU - Zhen,Jiesheng, AU - Niu,Zhili, AU - Li,Dong, AU - Liu,Fang, AU - Wu,Kailang, AU - Luo,Zhen, AU - Zhu,Chengliang, Y1 - 2020/06/26/ PY - 2020/04/30/received PY - 2020/06/08/revised PY - 2020/06/24/accepted PY - 2020/7/4/pubmed PY - 2020/8/25/medline PY - 2020/7/4/entrez KW - COVID-19 KW - IgM-IgG antibody test KW - Nucleic acid test KW - SARS-CoV-2 KW - Severity of illness SP - 106746 EP - 106746 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 86 N2 - BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused coronavirus disease 2019 (COVID-19) epidemic in China, December 2019. The clinical features and treatment of COVID-19 patients remain largely elusive. However, accurate detection is required for SARS-CoV-2 infection diagnosis. We aimed to evaluate the antibodies-based test and nucleic acid-based test for SARS-CoV-2-infected patients. METHODS: We retrospectively studied 133 patients diagnosed with SARS-CoV-2 and admitted to Renmin Hospital of Wuhan University, China, from January 23 to March 1, 2020. Demographic data, clinical records, laboratory tests, and outcomes were collected. Data were accessed by SARS-CoV-2 IgM-IgG antibody test and real-time reverse transcriptase PCR (RT-PCR) detection for SARS-CoV-2 nucleic acid in COVID-19 patients. RESULTS: Of 133 COVID-19 patients, there were 44 moderate cases, 52 severe cases, and 37 critical cases with no differences in gender and age among three subgroups. In RT-PCR detection, the positive rate was 65.9%, 71.2%, and 67.6% in moderate, severe, and critical cases, respectively. Whereas the positive rate of IgM/IgG antibody detection in patients was 79.5%/93.2%, 82.7%/100%, and 73.0%/97.3% in moderate, severe, and critical cases, respectively. Moreover, the IgM and IgG antibodies concentrations were also examined with no differences among three subgroups. CONCLUSION: The IgM-IgG antibody test exhibited a useful adjunct to RT-PCR detection, and improved the accuracy in COVID-19 diagnosis regardless of the severity of illness, which provides an effective complement to the false-negative results from a nucleic acid test for SARS-CoV-2 infection diagnosis after onsets. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/32619956/Analysis_of_adjunctive_serological_detection_to_nucleic_acid_test_for_severe_acute_respiratory_syndrome_coronavirus_2__SARS_CoV_2__infection_diagnosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(20)31369-2 DB - PRIME DP - Unbound Medicine ER -