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Analysis of lncRNAs-miRNAs-mRNAs networks in periodontal ligament stem cells under mechanical force.
Oral Dis. 2020 Jul 03 [Online ahead of print]OD

Abstract

OBJECTIVES

Our study aims to analyze the expression profiles of long non-coding RNAs (lncRNAs) and investigate the potential regulatory networks among lncRNAs, microRNAs (miRNAs), and mRNAs in periodontal ligament stem cells (PDLSCs) under mechanical force (MF).

MATERIALS AND METHODS

PDLSCs were isolated from human periodontal ligament tissues and identified by flow cytometry analysis. Multidirectional differentiation potential of PDLSCs was obtained by osteogenic and adipogenic induction. High-throughput RNA sequencing was used to identify the expression patterns of lncRNAs and mRNAs in PDLSCs under MF. MF-responsive miRNAs were obtained from the previous microarray data. LncRNAs-miRNAs-mRNAs networks were constructed by Cytoscape.

RESULTS

PDLSCs cultured from the periodontal ligament tissues were positive for STRO-1, CD146 and negative for CD45, CD34. Alizarin red staining and Oil Red O staining showed that PDLSCs had the ability of osteogenic and adipogenic differentiation. Then, a total of 1,339 and 1,426 differentially expressed lncRNAs and mRNAs were identified, respectively, in PDLSCs under MF. Based on the previous miRNA microarray analysis, the potential interaction networks of lncRNAs-miRNAs-mRNAs were constructed. It was found that lncRNAs and mRNAs could competitively interact with the same miRNA.

CONCLUSIONS

LncRNAs-miRNAs-mRNAs networks were involved in PDLSCs under MF, which might provide a novel mechanism in the regulation of clinical orthodontic tooth movement process.

Authors+Show Affiliations

Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.Jinan Hospital for Nationalities, Jinan, China.Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32620026

Citation

Wang, Hong, et al. "Analysis of lncRNAs-miRNAs-mRNAs Networks in Periodontal Ligament Stem Cells Under Mechanical Force." Oral Diseases, 2020.
Wang H, Feng C, Li M, et al. Analysis of lncRNAs-miRNAs-mRNAs networks in periodontal ligament stem cells under mechanical force. Oral Dis. 2020.
Wang, H., Feng, C., Li, M., Zhang, Z., Liu, J., & Wei, F. (2020). Analysis of lncRNAs-miRNAs-mRNAs networks in periodontal ligament stem cells under mechanical force. Oral Diseases. https://doi.org/10.1111/odi.13530
Wang H, et al. Analysis of lncRNAs-miRNAs-mRNAs Networks in Periodontal Ligament Stem Cells Under Mechanical Force. Oral Dis. 2020 Jul 3; PubMed PMID: 32620026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of lncRNAs-miRNAs-mRNAs networks in periodontal ligament stem cells under mechanical force. AU - Wang,Hong, AU - Feng,Cheng, AU - Li,Mengying, AU - Zhang,Zijie, AU - Liu,Jiani, AU - Wei,Fulan, Y1 - 2020/07/03/ PY - 2019/09/21/received PY - 2020/03/15/revised PY - 2020/06/19/accepted PY - 2020/7/4/pubmed PY - 2020/7/4/medline PY - 2020/7/4/entrez KW - long non-coding RNAs KW - mechanical force KW - orthodontic tooth movement KW - periodontal ligament stem cells JF - Oral diseases JO - Oral Dis N2 - OBJECTIVES: Our study aims to analyze the expression profiles of long non-coding RNAs (lncRNAs) and investigate the potential regulatory networks among lncRNAs, microRNAs (miRNAs), and mRNAs in periodontal ligament stem cells (PDLSCs) under mechanical force (MF). MATERIALS AND METHODS: PDLSCs were isolated from human periodontal ligament tissues and identified by flow cytometry analysis. Multidirectional differentiation potential of PDLSCs was obtained by osteogenic and adipogenic induction. High-throughput RNA sequencing was used to identify the expression patterns of lncRNAs and mRNAs in PDLSCs under MF. MF-responsive miRNAs were obtained from the previous microarray data. LncRNAs-miRNAs-mRNAs networks were constructed by Cytoscape. RESULTS: PDLSCs cultured from the periodontal ligament tissues were positive for STRO-1, CD146 and negative for CD45, CD34. Alizarin red staining and Oil Red O staining showed that PDLSCs had the ability of osteogenic and adipogenic differentiation. Then, a total of 1,339 and 1,426 differentially expressed lncRNAs and mRNAs were identified, respectively, in PDLSCs under MF. Based on the previous miRNA microarray analysis, the potential interaction networks of lncRNAs-miRNAs-mRNAs were constructed. It was found that lncRNAs and mRNAs could competitively interact with the same miRNA. CONCLUSIONS: LncRNAs-miRNAs-mRNAs networks were involved in PDLSCs under MF, which might provide a novel mechanism in the regulation of clinical orthodontic tooth movement process. SN - 1601-0825 UR - https://www.unboundmedicine.com/medline/citation/32620026/Analysis_of_lncRNAs-miRNAs-mRNAs_networks_in_periodontal_ligament_stem_cells_under_mechanical_force L2 - https://doi.org/10.1111/odi.13530 DB - PRIME DP - Unbound Medicine ER -
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