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Visualising virulence factors: Trichophyton benhamiaes subtilisins demonstrated in a guinea pig skin ex vivo model.
Mycoses. 2020 Jul 03 [Online ahead of print]M

Abstract

BACKGROUND

Dermatophytoses rank among the most frequent communicable diseases in humans, and the zoonotic transmission is increasing. The zoophilic dermatophyte Trichophyton (T.) benhamiae is nowadays one of the main causes of tinea faciei et corporis in children. However, scientific data on molecular pathomechanisms and specific virulence factors enabling this ubiquitous occurrence are scarce.

OBJECTIVES

To study tissue invasion and the expression of important virulence factors of T. benhamiae, isolates that were recovered from two groups of hosts (humans vs. guinea pigs (GP)) using an ex vivo skin model.

METHODS

After confirmation of species identity by ITS sequencing, CFU suspensions of dermatophyte isolates (n = 20) were applied to the skin infection model and cultured. Employing specific immunofluorescence staining techniques, the expression of subtilisin 3 and 6 and metallocarboxypeptidase A was analysed. The general mode of invasion was explored. Results were compared with biopsies of naturally infected GP.

RESULTS

All isolates were successfully recovered and proliferated well after application to the infection model. Progressive invasion of hyphae through all skin structures and destruction of explants were observed with early events being comparable to natural infection. An increasing expression of the examined virulence factors towards the end of culture was noticed but no difference between the two groups of isolates.

CONCLUSIONS

For the first time, important in vivo markers of dermatophytosis were visualised immunohistochemically in an ex vivo skin infection model and in skin biopsies of GP naturally infected with T. benhamiae. More research on the underlying pathomechanisms of dermatophyte infection is urgently needed.

Authors+Show Affiliations

Institute of Bacteriology and Mycology, Centre of Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.Institute of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.Laboratory for Medical Microbiology, Mölbis, Germany.Laboratory for Medical Microbiology, Mölbis, Germany.Institute of Bacteriology and Mycology, Centre of Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32620041

Citation

Baumbach, Christina-Marie, et al. "Visualising Virulence Factors: Trichophyton Benhamiaes Subtilisins Demonstrated in a Guinea Pig Skin Ex Vivo Model." Mycoses, 2020.
Baumbach CM, Michler JK, Nenoff P, et al. Visualising virulence factors: Trichophyton benhamiaes subtilisins demonstrated in a guinea pig skin ex vivo model. Mycoses. 2020.
Baumbach, C. M., Michler, J. K., Nenoff, P., Uhrlaβ, S., & Schrödl, W. (2020). Visualising virulence factors: Trichophyton benhamiaes subtilisins demonstrated in a guinea pig skin ex vivo model. Mycoses. https://doi.org/10.1111/myc.13136
Baumbach CM, et al. Visualising Virulence Factors: Trichophyton Benhamiaes Subtilisins Demonstrated in a Guinea Pig Skin Ex Vivo Model. Mycoses. 2020 Jul 3; PubMed PMID: 32620041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visualising virulence factors: Trichophyton benhamiaes subtilisins demonstrated in a guinea pig skin ex vivo model. AU - Baumbach,Christina-Marie, AU - Michler,Jule Kristin, AU - Nenoff,Pietro, AU - Uhrlaβ,Silke, AU - Schrödl,Wieland, Y1 - 2020/07/03/ PY - 2019/12/28/received PY - 2020/06/22/revised PY - 2020/06/26/accepted PY - 2020/7/4/pubmed PY - 2020/7/4/medline PY - 2020/7/4/entrez KW - Trichophyton benhamiae KW - ex vivo model KW - guinea pig skin explants KW - immunofluorescence KW - metallocarboxypeptidase A KW - subtilisins KW - virulence factors KW - zoonotic dermatophytes JF - Mycoses JO - Mycoses N2 - BACKGROUND: Dermatophytoses rank among the most frequent communicable diseases in humans, and the zoonotic transmission is increasing. The zoophilic dermatophyte Trichophyton (T.) benhamiae is nowadays one of the main causes of tinea faciei et corporis in children. However, scientific data on molecular pathomechanisms and specific virulence factors enabling this ubiquitous occurrence are scarce. OBJECTIVES: To study tissue invasion and the expression of important virulence factors of T. benhamiae, isolates that were recovered from two groups of hosts (humans vs. guinea pigs (GP)) using an ex vivo skin model. METHODS: After confirmation of species identity by ITS sequencing, CFU suspensions of dermatophyte isolates (n = 20) were applied to the skin infection model and cultured. Employing specific immunofluorescence staining techniques, the expression of subtilisin 3 and 6 and metallocarboxypeptidase A was analysed. The general mode of invasion was explored. Results were compared with biopsies of naturally infected GP. RESULTS: All isolates were successfully recovered and proliferated well after application to the infection model. Progressive invasion of hyphae through all skin structures and destruction of explants were observed with early events being comparable to natural infection. An increasing expression of the examined virulence factors towards the end of culture was noticed but no difference between the two groups of isolates. CONCLUSIONS: For the first time, important in vivo markers of dermatophytosis were visualised immunohistochemically in an ex vivo skin infection model and in skin biopsies of GP naturally infected with T. benhamiae. More research on the underlying pathomechanisms of dermatophyte infection is urgently needed. SN - 1439-0507 UR - https://www.unboundmedicine.com/medline/citation/32620041/Visualising_Virulence_Factors:_Trichophyton_benhamiaes_Subtilisins_demonstrated_in_a_Guinea_Pig_Skin_ex_vivo_Model L2 - https://doi.org/10.1111/myc.13136 DB - PRIME DP - Unbound Medicine ER -
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