Impact of CD44 gene single nucleotide polymorphism (rs 13347) in mobilization of autologous HSCT.Transfus Apher Sci. 2020 Jun 27 [Online ahead of print]TA
MOBILIZATION: of stem cells into peripheral blood is a crucial step in the procedure of autologous stem cell transplantation. Mobilization can be affected by many variables;underlying diseases, prior treatment and age. Many genetic polymorphisms mainly in adhesion molecules are thought to affect mobilization success. The CD44 is a cell adhesion molecule which is highly heterogeneous structurally. Polymorphisms in this molecule may impair HSC lodgment in the bone marrow.
THE AIM OF THE WORK
was to assess the impact of CD44 single nucleotide polymorphism (rs13347) on the efficacy of mobilization of CD34+ hematopoietic progenitor cells in a cohort of Egyptian patients treated for lymphoma and multiple myeloma and scheduled for autologous stem cell transplantation.
PATIENTS AND METHODS
the study was conducted on 92 patients scheduled for autologous HSC mobilization and transplantation. SNP genotyping was done by 5 ꞌ nuclease assay on rotor gene.
A statistical significant difference was detected between the patients with genotypes (CT + CC) and patients with (TT) genotype regarding the number of apheresis sessions required to gather the target CD34 count (p = 0.006).T allele is implicated in poorer mobilization.
CD44 SNPs analysis may be helpful for predicting the poor mobilizers. These patients may benefit from newer modalities like adhesion molecules inhibitors.