Tags

Type your tag names separated by a space and hit enter

Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease.
Sci Immunol. 2020 Jul 03; 5(49)SI

Abstract

Enterovirus D68 (EV-D68) causes outbreaks of respiratory illness, and there is increasing evidence that it causes outbreaks of acute flaccid myelitis (AFM). There are no licensed therapies to prevent or treat EV-D68 infection or AFM disease. We isolated a panel of EV-D68-reactive human monoclonal antibodies that recognize diverse antigenic variants from participants with prior infection. One potently neutralizing cross-reactive antibody, EV68-228, protected mice from respiratory and neurologic disease when given either before or after infection. Cryo-electron microscopy studies revealed that EV68-228 and another potently neutralizing antibody (EV68-159) bound around the fivefold or threefold axes of symmetry on virion particles, respectively. The structures suggest diverse mechanisms of action by these antibodies. The high potency and effectiveness observed in vivo suggest that antibodies are a mechanistic correlate of protection against AFM disease and are candidates for clinical use in humans with EV-D68 infection.

Authors+Show Affiliations

Department of Pediatrics (Infectious Diseases), Vanderbilt University Medical Center, Nashville, TN, USA.Department of Biological Sciences and Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA.Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA.Program in Chemical and Physical Biology, Vanderbilt University, Nashville, TN, USA.Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA. Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.Department of Biological Sciences and Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.Department of Biological Sciences and Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA.Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA. Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA.Department of Biological Sciences and Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.Department of Pediatrics (Infectious Diseases), Vanderbilt University Medical Center, Nashville, TN, USA. james.crowe@vumc.org. Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA. Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA. Program in Chemical and Physical Biology, Vanderbilt University, Nashville, TN, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32620559

Citation

Vogt, Matthew R., et al. "Human Antibodies Neutralize Enterovirus D68 and Protect Against Infection and Paralytic Disease." Science Immunology, vol. 5, no. 49, 2020.
Vogt MR, Fu J, Kose N, et al. Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease. Sci Immunol. 2020;5(49).
Vogt, M. R., Fu, J., Kose, N., Williamson, L. E., Bombardi, R., Setliff, I., Georgiev, I. S., Klose, T., Rossmann, M. G., Bochkov, Y. A., Gern, J. E., Kuhn, R. J., & Crowe, J. E. (2020). Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease. Science Immunology, 5(49). https://doi.org/10.1126/sciimmunol.aba4902
Vogt MR, et al. Human Antibodies Neutralize Enterovirus D68 and Protect Against Infection and Paralytic Disease. Sci Immunol. 2020 Jul 3;5(49) PubMed PMID: 32620559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease. AU - Vogt,Matthew R, AU - Fu,Jianing, AU - Kose,Nurgun, AU - Williamson,Lauren E, AU - Bombardi,Robin, AU - Setliff,Ian, AU - Georgiev,Ivelin S, AU - Klose,Thomas, AU - Rossmann,Michael G, AU - Bochkov,Yury A, AU - Gern,James E, AU - Kuhn,Richard J, AU - Crowe,James E,Jr PY - 2019/12/08/received PY - 2020/06/12/accepted PY - 2020/7/5/entrez PY - 2020/7/6/pubmed PY - 2020/7/6/medline JF - Science immunology JO - Sci Immunol VL - 5 IS - 49 N2 - Enterovirus D68 (EV-D68) causes outbreaks of respiratory illness, and there is increasing evidence that it causes outbreaks of acute flaccid myelitis (AFM). There are no licensed therapies to prevent or treat EV-D68 infection or AFM disease. We isolated a panel of EV-D68-reactive human monoclonal antibodies that recognize diverse antigenic variants from participants with prior infection. One potently neutralizing cross-reactive antibody, EV68-228, protected mice from respiratory and neurologic disease when given either before or after infection. Cryo-electron microscopy studies revealed that EV68-228 and another potently neutralizing antibody (EV68-159) bound around the fivefold or threefold axes of symmetry on virion particles, respectively. The structures suggest diverse mechanisms of action by these antibodies. The high potency and effectiveness observed in vivo suggest that antibodies are a mechanistic correlate of protection against AFM disease and are candidates for clinical use in humans with EV-D68 infection. SN - 2470-9468 UR - https://www.unboundmedicine.com/medline/citation/32620559/Human_antibodies_neutralize_enterovirus_D68_and_protect_against_infection_and_paralytic_disease L2 - http://immunology.sciencemag.org/cgi/pmidlookup?view=long&pmid=32620559 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.