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Transcriptome analysis of testis reveals the effects of developmental exposure to bisphenol a or 17α-ethinylestradiol in medaka (Oryzias latipes).
Aquat Toxicol. 2020 Aug; 225:105553.AT

Abstract

Endocrine disrupting chemicals (EDCs) can induce abnormalities in organisms via alteration of molecular pathways and subsequent disruption of endocrine functions. Bisphenol A (BPA) and 17α-ethinylestradiol (EE2) are ubiquitous EDCs in the environment. Many aquatic organisms, including fish, are often exposed to varying concentrations of BPA and EE2 throughout their lifespan. Both BPA and EE2 can activate estrogenic signaling pathways and cause adverse effects on reproduction via alteration of pathways associated with steroidogenesis. However, transcriptional pathways that are affected by chronic exposure to these two ubiquitous environmental estrogens during embryonic, larval, and juvenile stages are not clearly understood. In the present study, we examined transcriptional alterations in the testis of medaka fish (Oryzias latipes) chronically exposed to a low concentration of BPA or EE2. Medaka were exposed to BPA (10 μg/L) or EE2 (0.01 μg/L) from 8 h post-fertilization (as embryos) to adulthood 50 days post fertilization (dpf), and transcriptional alterations in the testis were examined by RNA sequencing (RNA-seq). Transcriptomic profiling revealed 651 differentially expressed genes (DEGs) between BPA-exposed and control testes, while 1475 DEGs were found between EE2-exposed and control testes. Gene ontology (GO) analysis showed a significant enrichment of "intracellular receptor signaling pathway", "response to steroid hormone" and "hormone-mediated signaling pathway" in the BPA-induced DEGs, and of "cilium organization", "microtubule-based process" and "organelle assembly" in the EE2-induced DEGs. Pathway analysis showed significant enrichment of "integrin signaling pathway" in both treatment groups, and of "cadherin signaling pathway", "Alzheimer disease-presenilin pathway" in EE2-induced DEGs. Single nucleotide polymorphism (SNP) and insertion-deletion (Indel) analysis found no significant differences in mutation rates with either BPA or EE2 treatments. Taken together, global gene expression differences in testes of medaka during early stages of gametogenesis were responsive to chronic BPA and EE2 exposure.

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Authors+Show Affiliations

Bhandari RK
Biology Department, University of North Carolina Greensboro, Greensboro, NC 27412, United States; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, United States; United States Geological Survey, Columbia Environmental Research Center, Columbia, MO 65201, United States. Electronic address: rkbhanda@uncg.edu.
Wang X
Biology Department, University of North Carolina Greensboro, Greensboro, NC 27412, United States.
Saal FSV
Division of Biological Sciences, University of Missouri, Columbia, MO 65211, United States.
Tillitt DE
United States Geological Survey, Columbia Environmental Research Center, Columbia, MO 65201, United States.

MeSH

AnimalsBenzhydryl CompoundsEndocrine DisruptorsEstrogensEthinyl EstradiolFemaleGene Expression ProfilingLarvaMaleOryziasPhenolsReproductionTestisWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32622090

Citation

Bhandari, Ramji K., et al. "Transcriptome Analysis of Testis Reveals the Effects of Developmental Exposure to Bisphenol a or 17α-ethinylestradiol in Medaka (Oryzias Latipes)." Aquatic Toxicology (Amsterdam, Netherlands), vol. 225, 2020, p. 105553.
Bhandari RK, Wang X, Saal FSV, et al. Transcriptome analysis of testis reveals the effects of developmental exposure to bisphenol a or 17α-ethinylestradiol in medaka (Oryzias latipes). Aquat Toxicol. 2020;225:105553.
Bhandari, R. K., Wang, X., Saal, F. S. V., & Tillitt, D. E. (2020). Transcriptome analysis of testis reveals the effects of developmental exposure to bisphenol a or 17α-ethinylestradiol in medaka (Oryzias latipes). Aquatic Toxicology (Amsterdam, Netherlands), 225, 105553. https://doi.org/10.1016/j.aquatox.2020.105553
Bhandari RK, et al. Transcriptome Analysis of Testis Reveals the Effects of Developmental Exposure to Bisphenol a or 17α-ethinylestradiol in Medaka (Oryzias Latipes). Aquat Toxicol. 2020;225:105553. PubMed PMID: 32622090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis of testis reveals the effects of developmental exposure to bisphenol a or 17α-ethinylestradiol in medaka (Oryzias latipes). AU - Bhandari,Ramji K, AU - Wang,Xuegeng, AU - Saal,Frederick S Vom, AU - Tillitt,Donald E, Y1 - 2020/06/24/ PY - 2019/11/20/received PY - 2020/05/19/revised PY - 2020/06/21/accepted PY - 2020/7/6/pubmed PY - 2020/8/6/medline PY - 2020/7/5/entrez KW - BPA KW - Chronic exposure KW - EE2 KW - Gametogenesis KW - Male KW - Medaka fish KW - RNA Sequencing SP - 105553 EP - 105553 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 225 N2 - Endocrine disrupting chemicals (EDCs) can induce abnormalities in organisms via alteration of molecular pathways and subsequent disruption of endocrine functions. Bisphenol A (BPA) and 17α-ethinylestradiol (EE2) are ubiquitous EDCs in the environment. Many aquatic organisms, including fish, are often exposed to varying concentrations of BPA and EE2 throughout their lifespan. Both BPA and EE2 can activate estrogenic signaling pathways and cause adverse effects on reproduction via alteration of pathways associated with steroidogenesis. However, transcriptional pathways that are affected by chronic exposure to these two ubiquitous environmental estrogens during embryonic, larval, and juvenile stages are not clearly understood. In the present study, we examined transcriptional alterations in the testis of medaka fish (Oryzias latipes) chronically exposed to a low concentration of BPA or EE2. Medaka were exposed to BPA (10 μg/L) or EE2 (0.01 μg/L) from 8 h post-fertilization (as embryos) to adulthood 50 days post fertilization (dpf), and transcriptional alterations in the testis were examined by RNA sequencing (RNA-seq). Transcriptomic profiling revealed 651 differentially expressed genes (DEGs) between BPA-exposed and control testes, while 1475 DEGs were found between EE2-exposed and control testes. Gene ontology (GO) analysis showed a significant enrichment of "intracellular receptor signaling pathway", "response to steroid hormone" and "hormone-mediated signaling pathway" in the BPA-induced DEGs, and of "cilium organization", "microtubule-based process" and "organelle assembly" in the EE2-induced DEGs. Pathway analysis showed significant enrichment of "integrin signaling pathway" in both treatment groups, and of "cadherin signaling pathway", "Alzheimer disease-presenilin pathway" in EE2-induced DEGs. Single nucleotide polymorphism (SNP) and insertion-deletion (Indel) analysis found no significant differences in mutation rates with either BPA or EE2 treatments. Taken together, global gene expression differences in testes of medaka during early stages of gametogenesis were responsive to chronic BPA and EE2 exposure. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/32622090/Transcriptome_analysis_of_testis_reveals_the_effects_of_developmental_exposure_to_bisphenol_a_or_17α_ethinylestradiol_in_medaka__Oryzias_latipes__ DB - PRIME DP - Unbound Medicine ER -