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Recovery of aerobic gram-negative bacteria from the Copan Eswab transport system after long-term storage.
Diagn Microbiol Infect Dis. 2020 Jun 06; 98(1):115100.DM

Abstract

We evaluated the Copan Eswab transport system for the quantitative recovery of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa after 1, 2, 3, 5, and 7 days of storage at room and refrigerator temperatures, and 7 and 30 days of storage at -80 °C and -20 °C using mono- and polymicrobial samples. The study was based on Clinical and Laboratory Standards Institute (CLSI) M40-A2 standard procedures on the quality control of microbiological transport systems. Eswab met the CLSI standards at room and refrigerator temperatures for all (combinations of) bacterial strains tested. At room temperature, after 24 h, bacterial growth was observed. At -80 °C, bacterial viability was maintained in monomicrobial samples; however, in polymicrobial samples, P. aeruginosa recovery was compromised. Storage at -20 °C was unsuitable. We conclude that specimens collected using Eswab should be transported to the laboratory as soon as possible. If transport or processing is delayed, specimens should preferably be stored at refrigerator temperatures.

Authors+Show Affiliations

Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands. Electronic address: sofie.tops@radboudumc.nl.Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands. Electronic address: kolmus94@gmail.com.Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands. Electronic address: Damy.Wulms@student.ru.nl.Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands. Electronic address: Jakko.vanIngen@radboudumc.nl.Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands. Electronic address: Heiman.Wertheim@radboudumc.nl.Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands. Electronic address: Eva.Kolwijck@radboudumc.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32622288

Citation

Tops, Sofie C M., et al. "Recovery of Aerobic Gram-negative Bacteria From the Copan Eswab Transport System After Long-term Storage." Diagnostic Microbiology and Infectious Disease, vol. 98, no. 1, 2020, p. 115100.
Tops SCM, Kolmus M, Wulms D, et al. Recovery of aerobic gram-negative bacteria from the Copan Eswab transport system after long-term storage. Diagn Microbiol Infect Dis. 2020;98(1):115100.
Tops, S. C. M., Kolmus, M., Wulms, D., van Ingen, J., Wertheim, H. F. L., & Kolwijck, E. (2020). Recovery of aerobic gram-negative bacteria from the Copan Eswab transport system after long-term storage. Diagnostic Microbiology and Infectious Disease, 98(1), 115100. https://doi.org/10.1016/j.diagmicrobio.2020.115100
Tops SCM, et al. Recovery of Aerobic Gram-negative Bacteria From the Copan Eswab Transport System After Long-term Storage. Diagn Microbiol Infect Dis. 2020 Jun 6;98(1):115100. PubMed PMID: 32622288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recovery of aerobic gram-negative bacteria from the Copan Eswab transport system after long-term storage. AU - Tops,Sofie C M, AU - Kolmus,Marlien, AU - Wulms,Damy, AU - van Ingen,Jakko, AU - Wertheim,Heiman F L, AU - Kolwijck,Eva, Y1 - 2020/06/06/ PY - 2020/02/12/received PY - 2020/05/27/revised PY - 2020/05/29/accepted PY - 2020/7/6/pubmed PY - 2020/7/6/medline PY - 2020/7/5/entrez KW - Bacterial recovery KW - Enterobacterales KW - Microbial colony count KW - Microbial viability KW - Pseudomonas aeruginosa KW - Swab transport system SP - 115100 EP - 115100 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 98 IS - 1 N2 - We evaluated the Copan Eswab transport system for the quantitative recovery of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa after 1, 2, 3, 5, and 7 days of storage at room and refrigerator temperatures, and 7 and 30 days of storage at -80 °C and -20 °C using mono- and polymicrobial samples. The study was based on Clinical and Laboratory Standards Institute (CLSI) M40-A2 standard procedures on the quality control of microbiological transport systems. Eswab met the CLSI standards at room and refrigerator temperatures for all (combinations of) bacterial strains tested. At room temperature, after 24 h, bacterial growth was observed. At -80 °C, bacterial viability was maintained in monomicrobial samples; however, in polymicrobial samples, P. aeruginosa recovery was compromised. Storage at -20 °C was unsuitable. We conclude that specimens collected using Eswab should be transported to the laboratory as soon as possible. If transport or processing is delayed, specimens should preferably be stored at refrigerator temperatures. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/32622288/Recovery_of_aerobic_gram-negative_bacteria_from_the_Copan_Eswab_transport_system_after_long-term_storage L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(20)30477-6 DB - PRIME DP - Unbound Medicine ER -
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