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The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046.
Int J Biol Macromol. 2020 Jul 03; 163:240-250.IJ

Abstract

Reconstruction of genome-based metabolic model is a useful approach for the assessment of metabolic pathways, genes and proteins involved in the environmental fitness capabilities or pathogenic potential as well as for biotechnological processes development. Pseudomonas sp. LFM046 was selected as a good polyhydroxyalkanoates (PHA) producer from carbohydrates and plant oils. Its complete genome sequence and metabolic model were obtained. Analysis revealed that the gnd gene, encoding 6-phosphogluconate dehydrogenase, is absent in Pseudomonas sp. LFM046 genome. In order to improve the knowledge about LFM046 metabolism, the coenzyme specificities of different enzymes was evaluated. Furthermore, the heterologous expression of gnd genes from Pseudomonas putida KT2440 (NAD+ dependent) and Escherichia coli MG1655 (NADP+ dependent) in LFM046 was carried out and provoke a delay on cell growth and a reduction in PHA yield, respectively. The results indicate that the adjustment in cyclic Entner-Doudoroff pathway may be an interesting strategy for it and other bacteria to simultaneously meet divergent cell needs during cultivation phases of growth and PHA production.

Authors+Show Affiliations

University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil; Westfalische Wilhelms-Universitat Münster, Institut für Molekulare Mikrobiologie und Biotechnologie, Corrensstrasse 3, D-48149 Münster, Germany. Electronic address: jcardinali@usp.br.ERABLE Team, Inria Grenoble Rhône-Alpes, Université de Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR 5558, F-69622 Villeurbanne, France.University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil.Westfalische Wilhelms-Universitat Münster, Institut für Molekulare Mikrobiologie und Biotechnologie, Corrensstrasse 3, D-48149 Münster, Germany; Environmental Sciences Department, King Abdulaziz University, Jeddah, Saudi Arabia.ERABLE Team, Inria Grenoble Rhône-Alpes, Université de Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR 5558, F-69622 Villeurbanne, France.IDMEC, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal; REQUIMTE/LAQV, Department of Chemistry, Faculty of Science and Technology, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil.University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil.University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil.University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil. Electronic address: jgcgomez@usp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32622773

Citation

Cardinali-Rezende, Juliana, et al. "The Relevance of Enzyme Specificity for Coenzymes and the Presence of 6-phosphogluconate Dehydrogenase for Polyhydroxyalkanoates Production in the Metabolism of Pseudomonas Sp. LFM046." International Journal of Biological Macromolecules, vol. 163, 2020, pp. 240-250.
Cardinali-Rezende J, Di Genova A, Nahat RATPS, et al. The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046. Int J Biol Macromol. 2020;163:240-250.
Cardinali-Rezende, J., Di Genova, A., Nahat, R. A. T. P. S., Steinbüchel, A., Sagot, M. F., Costa, R. S., Oliveira, H. C., Taciro, M. K., Silva, L. F., & Gomez, J. G. C. (2020). The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046. International Journal of Biological Macromolecules, 163, 240-250. https://doi.org/10.1016/j.ijbiomac.2020.06.226
Cardinali-Rezende J, et al. The Relevance of Enzyme Specificity for Coenzymes and the Presence of 6-phosphogluconate Dehydrogenase for Polyhydroxyalkanoates Production in the Metabolism of Pseudomonas Sp. LFM046. Int J Biol Macromol. 2020 Jul 3;163:240-250. PubMed PMID: 32622773.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046. AU - Cardinali-Rezende,Juliana, AU - Di Genova,Alex, AU - Nahat,Rafael A T P S, AU - Steinbüchel,Alexander, AU - Sagot,Marie-France, AU - Costa,Rafael S, AU - Oliveira,Henrique C, AU - Taciro,Marilda K, AU - Silva,Luiziana F, AU - Gomez,José Gregório C, Y1 - 2020/07/03/ PY - 2020/03/19/received PY - 2020/06/14/revised PY - 2020/06/24/accepted PY - 2020/7/6/pubmed PY - 2020/7/6/medline PY - 2020/7/6/entrez KW - 6-phosphogluconate dehydrogenase KW - Entner-Doudoroff pathway KW - PHA production KW - Pentose phosphate pathway KW - Pseudomonas sp. KW - Reconstruction of genome-based metabolic model SP - 240 EP - 250 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 163 N2 - Reconstruction of genome-based metabolic model is a useful approach for the assessment of metabolic pathways, genes and proteins involved in the environmental fitness capabilities or pathogenic potential as well as for biotechnological processes development. Pseudomonas sp. LFM046 was selected as a good polyhydroxyalkanoates (PHA) producer from carbohydrates and plant oils. Its complete genome sequence and metabolic model were obtained. Analysis revealed that the gnd gene, encoding 6-phosphogluconate dehydrogenase, is absent in Pseudomonas sp. LFM046 genome. In order to improve the knowledge about LFM046 metabolism, the coenzyme specificities of different enzymes was evaluated. Furthermore, the heterologous expression of gnd genes from Pseudomonas putida KT2440 (NAD+ dependent) and Escherichia coli MG1655 (NADP+ dependent) in LFM046 was carried out and provoke a delay on cell growth and a reduction in PHA yield, respectively. The results indicate that the adjustment in cyclic Entner-Doudoroff pathway may be an interesting strategy for it and other bacteria to simultaneously meet divergent cell needs during cultivation phases of growth and PHA production. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/32622773/The_relevance_of_enzyme_specificity_for_coenzymes_and_the_presence_of_6-phosphogluconate_dehydrogenase_for_polyhydroxyalkanoates_production_in_the_metabolism_of_Pseudomonas_sp._LFM046 L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(20)33680-1 DB - PRIME DP - Unbound Medicine ER -
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