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Chemical composition and antimicrobial activity of cuticular and internal lipids of the insect Rhynchophorus palmarum.
Arch Insect Biochem Physiol. 2020 Jul 05 [Online ahead of print]AI

Abstract

Insect cuticle lipids are involved in various types of chemical communication between species, and reduce the penetration of insecticides, chemicals, and toxins, as well as provide protection against the attack of microorganisms, parasitic insects, and predators. Ecological studies related to the insect Rhynchophorus palmarum are well-known; however, very little is known about its resistance mechanisms, which includes its lipid composition and its importance, specifically the cuticle layer. This study aimed to characterize the cuticle and internal lipid compounds of the male and female R. palmarum adult insects and to evaluate the presence of antimicrobial activity. We performed by gas chromatography coupled to mass spectrometry (GC-MS) analyzes of lipid extracts fractions and we identified 10 methyl esters of fatty acids esters of C14 to C23, with variation between the sexes of C22:0, C21:0, present only in male cuticle, and C20:2 in female. The lipid content of this insect showed relevant amount of C16:1, C18:1, and C18:2. The antimicrobial activity of the cuticular and internal fractions obtained was tested, which resulted in minimum inhibitory concentrations between 12.5 and 20 μg/ml against Gram-positive bacteria (Staphylococcus epidermidis, Enterococcus faecalis), Gram-negative (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia), and fungal species (Candida albicans e Candida tropicalis). The antimicrobial effect of the R. palmarum cuticle open perspectives for a new source to bioinsecticidal strategies, in addition to elucidating a bioactive mixture against bacteria and fungi.

Authors+Show Affiliations

Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.Agrarian Sciences Center, Federal University of Alagoas, Maceió, Alagoas, Brazil.Agrarian Sciences Center, Federal University of Alagoas, Maceió, Alagoas, Brazil.Laboratory of Cell Biochemistry, Federal University of São João del Rei, Dona Lindú Centro-Oeste Campus, Divinópolis, Minas Gerais, Brazil.Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32623787

Citation

Batalha, Mariana de M C., et al. "Chemical Composition and Antimicrobial Activity of Cuticular and Internal Lipids of the Insect Rhynchophorus Palmarum." Archives of Insect Biochemistry and Physiology, 2020, pp. e21723.
Batalha MMC, Goulart HF, Santana AEG, et al. Chemical composition and antimicrobial activity of cuticular and internal lipids of the insect Rhynchophorus palmarum. Arch Insect Biochem Physiol. 2020.
Batalha, M. M. C., Goulart, H. F., Santana, A. E. G., Barbosa, L. A. O., Nascimento, T. G., da Silva, M. K. H., Dornelas, C. B., & Grillo, L. A. M. (2020). Chemical composition and antimicrobial activity of cuticular and internal lipids of the insect Rhynchophorus palmarum. Archives of Insect Biochemistry and Physiology, e21723. https://doi.org/10.1002/arch.21723
Batalha MMC, et al. Chemical Composition and Antimicrobial Activity of Cuticular and Internal Lipids of the Insect Rhynchophorus Palmarum. Arch Insect Biochem Physiol. 2020 Jul 5;e21723. PubMed PMID: 32623787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical composition and antimicrobial activity of cuticular and internal lipids of the insect Rhynchophorus palmarum. AU - Batalha,Mariana de M C, AU - Goulart,Henrique F, AU - Santana,Antônio E G, AU - Barbosa,Leandro A O, AU - Nascimento,Ticiano G, AU - da Silva,Meirielly K H, AU - Dornelas,Camila B, AU - Grillo,Luciano A M, Y1 - 2020/07/05/ PY - 2020/03/22/received PY - 2020/06/09/revised PY - 2020/06/20/accepted PY - 2020/7/6/entrez PY - 2020/7/6/pubmed PY - 2020/7/6/medline KW - R. palmarum KW - antimicrobial potential KW - cuticle KW - lipid profile SP - e21723 EP - e21723 JF - Archives of insect biochemistry and physiology JO - Arch. Insect Biochem. Physiol. N2 - Insect cuticle lipids are involved in various types of chemical communication between species, and reduce the penetration of insecticides, chemicals, and toxins, as well as provide protection against the attack of microorganisms, parasitic insects, and predators. Ecological studies related to the insect Rhynchophorus palmarum are well-known; however, very little is known about its resistance mechanisms, which includes its lipid composition and its importance, specifically the cuticle layer. This study aimed to characterize the cuticle and internal lipid compounds of the male and female R. palmarum adult insects and to evaluate the presence of antimicrobial activity. We performed by gas chromatography coupled to mass spectrometry (GC-MS) analyzes of lipid extracts fractions and we identified 10 methyl esters of fatty acids esters of C14 to C23, with variation between the sexes of C22:0, C21:0, present only in male cuticle, and C20:2 in female. The lipid content of this insect showed relevant amount of C16:1, C18:1, and C18:2. The antimicrobial activity of the cuticular and internal fractions obtained was tested, which resulted in minimum inhibitory concentrations between 12.5 and 20 μg/ml against Gram-positive bacteria (Staphylococcus epidermidis, Enterococcus faecalis), Gram-negative (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia), and fungal species (Candida albicans e Candida tropicalis). The antimicrobial effect of the R. palmarum cuticle open perspectives for a new source to bioinsecticidal strategies, in addition to elucidating a bioactive mixture against bacteria and fungi. SN - 1520-6327 UR - https://www.unboundmedicine.com/medline/citation/32623787/Chemical_composition_and_antimicrobial_activity_of_cuticular_and_internal_lipids_of_the_insect_Rhynchophorus_palmarum L2 - https://doi.org/10.1002/arch.21723 DB - PRIME DP - Unbound Medicine ER -
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