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Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients.
Dis Markers. 2020; 2020:9369341.DM

Abstract

Background

Thyroid carcinoma (THCA) is one of the most common malignancies of the endocrine system, which is usually treated by surgery combined with iodine-131 (I131) radiotherapy.

Aims

This study is aimed at exploring the potential targets of I131 radiotherapy in THCA.

Methods

The RNA-sequencing data of THCA in The Cancer Genome Atlas database (including 568 THCA samples) was downloaded. The differentially expressed genes (DEGs) between the tumour samples whether or not subjected to I131 radiotherapy were identified using edgeR package. Using the WGCNA package, the module that was relevant with I131 radiotherapy was selected. The intersection genes of the hub module nodes and the DEGs were obtained as hub genes, followed by the function and pathway enrichment analyses using the clusterProfiler package. Moreover, the protein-protein interaction (PPI) network for the hub genes was constructed using Cytoscape software. In addition, more important hub genes were analysed with function mining using the GenCLiP2 online tool. The qPCR analysis was used to verify the mRNA expression of more important hub genes in THCA tissues.

Results

There were 500 DEGs (167 upregulated and 333 downregulated) between the two groups. WGCNA analysis showed that the green module (428 nodes) exhibited the most significant correlation with I131 radiotherapy. A PPI network was built after the identification of 53 hub genes. In the PPI network, CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 exhibited higher degrees, which were mainly implicated in the vascular function. The relative expression of nine mRNAs in the THCA tissues treated with I131 was lower.

Conclusion

I131 radiotherapy might exert therapeutic effects by targeting CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 in THCA patients.

Authors+Show Affiliations

Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hosp Huzhou University, 198 Hongqi Rd, Huzhou, Zhejiang, China.Department of Oncology, Huzhou Central Hospital, 198 Hongqi Road, Huzhou, Zhejiang Province 313000, China.Department of Nursing, Huzhou Third Municipal Hospital, No. 2088 East Tiaoxi Road, Huzhou, Zhejiang Province 313000, China.Graduate School of Nursing, Huzhou University, No. 1 Bachelor Road, Huzhou, Zhejiang Province 313000, China.Graduate School of Nursing, Huzhou University, No. 1 Bachelor Road, Huzhou, Zhejiang Province 313000, China.Department of Thyroid Surgery, Huzhou Central Hospital, No. 198 Hongqi Road, Huzhou, Zhejiang Province 313000, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32626543

Citation

Shuwen, Han, et al. "Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients." Disease Markers, vol. 2020, 2020, p. 9369341.
Shuwen H, Xi Y, Miao D, et al. Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients. Dis Markers. 2020;2020:9369341.
Shuwen, H., Xi, Y., Miao, D., Jiamin, X., Jing, Z., & Weili, G. (2020). Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients. Disease Markers, 2020, 9369341. https://doi.org/10.1155/2020/9369341
Shuwen H, et al. Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients. Dis Markers. 2020;2020:9369341. PubMed PMID: 32626543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients. AU - Shuwen,Han, AU - Xi,Yang, AU - Miao,Da, AU - Jiamin,Xu, AU - Jing,Zhuang, AU - Weili,Gao, Y1 - 2020/06/16/ PY - 2019/08/19/received PY - 2020/06/02/accepted PY - 2020/7/7/entrez PY - 2020/7/7/pubmed PY - 2020/7/7/medline SP - 9369341 EP - 9369341 JF - Disease markers JO - Dis. Markers VL - 2020 N2 - Background: Thyroid carcinoma (THCA) is one of the most common malignancies of the endocrine system, which is usually treated by surgery combined with iodine-131 (I131) radiotherapy. Aims: This study is aimed at exploring the potential targets of I131 radiotherapy in THCA. Methods: The RNA-sequencing data of THCA in The Cancer Genome Atlas database (including 568 THCA samples) was downloaded. The differentially expressed genes (DEGs) between the tumour samples whether or not subjected to I131 radiotherapy were identified using edgeR package. Using the WGCNA package, the module that was relevant with I131 radiotherapy was selected. The intersection genes of the hub module nodes and the DEGs were obtained as hub genes, followed by the function and pathway enrichment analyses using the clusterProfiler package. Moreover, the protein-protein interaction (PPI) network for the hub genes was constructed using Cytoscape software. In addition, more important hub genes were analysed with function mining using the GenCLiP2 online tool. The qPCR analysis was used to verify the mRNA expression of more important hub genes in THCA tissues. Results: There were 500 DEGs (167 upregulated and 333 downregulated) between the two groups. WGCNA analysis showed that the green module (428 nodes) exhibited the most significant correlation with I131 radiotherapy. A PPI network was built after the identification of 53 hub genes. In the PPI network, CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 exhibited higher degrees, which were mainly implicated in the vascular function. The relative expression of nine mRNAs in the THCA tissues treated with I131 was lower. Conclusion: I131 radiotherapy might exert therapeutic effects by targeting CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 in THCA patients. SN - 1875-8630 UR - https://www.unboundmedicine.com/medline/citation/32626543/Nine_Genes_Mediate_the_Therapeutic_Effects_of_Iodine-131_Radiotherapy_in_Thyroid_Carcinoma_Patients L2 - https://doi.org/10.1155/2020/9369341 DB - PRIME DP - Unbound Medicine ER -
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