Tags

Type your tag names separated by a space and hit enter

Effects of Botulinum Toxin Type A on Microvessels in Hypertrophic Scar Models on Rabbit Ears.
Biomed Res Int. 2020; 2020:2170750.BR

Abstract

Background

Although Botulinum Toxin Type A (BTXA) has been applied to scar prevention and treatment, the mechanisms still require further exploration.

Objective

To investigate the effects of BTXA on microvessels in the hypertrophic scar models on rabbit ears.

Methods

Eight big-eared New Zealand rabbits (males or females) were selected to establish scar models. One ear of each rabbit (4 models in each ear) was selected randomly to be injected with BTXA immediately after modeling and included in the treated group, while the opposite ear was untreated and included in the control group. The growth of scars in each group was observed and recorded, and 4 rabbits were sacrificed on days 30 and 45 after modeling. Then, scar height was measured by hematoxylin-eosin (HE) staining, vascular endothelial growth factor (VEGF) expression was detected by immunohistochemical (IHC) testing, and microvessel density (MVD) was calculated based on CD34 (human hematopoietic progenitor cell antigen).

Results

The wounds in each group were well healed and free from infection or necrosis. On days 30 and 45, the scar height, MVD value, and VEGF expression in the treated group were lower than those in the control group (P < 0.05). For the treated group, the above indicators on day 45 were lower than on day 30 (P > 0.05). Besides, there was a positive correlation between the MVD value and the VEGF expression in the treated group (P < 0.05).

Conclusion

The injection of BTXA immediately after modeling inhibits VEGF expression and reduces angiogenesis, thereby inhibiting hypertrophic scar formation.

Authors+Show Affiliations

Aier School of Ophthalmology, Central South University, Changsha, China.Department of Oculoplastic Surgery, Hankou Aier Eye Hospital, Wuhan, China.Aier School of Ophthalmology, Central South University, Changsha, China.Aier School of Ophthalmology, Central South University, Changsha, China.Department of Oculoplastic Surgery, Hankou Aier Eye Hospital, Wuhan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32626735

Citation

Zhou, Na, et al. "Effects of Botulinum Toxin Type a On Microvessels in Hypertrophic Scar Models On Rabbit Ears." BioMed Research International, vol. 2020, 2020, p. 2170750.
Zhou N, Li D, Luo Y, et al. Effects of Botulinum Toxin Type A on Microvessels in Hypertrophic Scar Models on Rabbit Ears. Biomed Res Int. 2020;2020:2170750.
Zhou, N., Li, D., Luo, Y., Li, J., & Wang, Y. (2020). Effects of Botulinum Toxin Type A on Microvessels in Hypertrophic Scar Models on Rabbit Ears. BioMed Research International, 2020, 2170750. https://doi.org/10.1155/2020/2170750
Zhou N, et al. Effects of Botulinum Toxin Type a On Microvessels in Hypertrophic Scar Models On Rabbit Ears. Biomed Res Int. 2020;2020:2170750. PubMed PMID: 32626735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Botulinum Toxin Type A on Microvessels in Hypertrophic Scar Models on Rabbit Ears. AU - Zhou,Na, AU - Li,Dongping, AU - Luo,Yanzhu, AU - Li,Junping, AU - Wang,Yuhong, Y1 - 2020/06/16/ PY - 2020/02/17/received PY - 2020/05/26/accepted PY - 2020/7/7/entrez PY - 2020/7/7/pubmed PY - 2020/7/7/medline SP - 2170750 EP - 2170750 JF - BioMed research international JO - Biomed Res Int VL - 2020 N2 - Background: Although Botulinum Toxin Type A (BTXA) has been applied to scar prevention and treatment, the mechanisms still require further exploration. Objective: To investigate the effects of BTXA on microvessels in the hypertrophic scar models on rabbit ears. Methods: Eight big-eared New Zealand rabbits (males or females) were selected to establish scar models. One ear of each rabbit (4 models in each ear) was selected randomly to be injected with BTXA immediately after modeling and included in the treated group, while the opposite ear was untreated and included in the control group. The growth of scars in each group was observed and recorded, and 4 rabbits were sacrificed on days 30 and 45 after modeling. Then, scar height was measured by hematoxylin-eosin (HE) staining, vascular endothelial growth factor (VEGF) expression was detected by immunohistochemical (IHC) testing, and microvessel density (MVD) was calculated based on CD34 (human hematopoietic progenitor cell antigen). Results: The wounds in each group were well healed and free from infection or necrosis. On days 30 and 45, the scar height, MVD value, and VEGF expression in the treated group were lower than those in the control group (P < 0.05). For the treated group, the above indicators on day 45 were lower than on day 30 (P > 0.05). Besides, there was a positive correlation between the MVD value and the VEGF expression in the treated group (P < 0.05). Conclusion: The injection of BTXA immediately after modeling inhibits VEGF expression and reduces angiogenesis, thereby inhibiting hypertrophic scar formation. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/32626735/Effects_of_Botulinum_Toxin_Type_A_on_Microvessels_in_Hypertrophic_Scar_Models_on_Rabbit_Ears L2 - https://doi.org/10.1155/2020/2170750 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.