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Increased expression of RBMS3 predicts a favorable prognosis in human gallbladder carcinoma.
Oncol Rep. 2020 Jul; 44(1):55-68.OR

Abstract

Multiple regions in the short arm of chromosome 3 are frequently deleted in a variety of solid tumors including gallbladder carcinoma (GBC). RNA binding motif, single‑stranded interacting protein 3 (RBMS3), a tumor suppressor gene (TSG), is located in this region. However, the role of RBMS3 in GBC remains unclear. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting were performed to evaluate the mRNA and protein expression levels of RBMS3 in 41 fresh frozen GBC tissues and paired normal tissues. An immunohistochemical assay was performed on a tissue microarray (TMA, consisting of 125 cases GBC and 47 normal controls). Microvessel density (MVD) counts were determined using CD34 immunohistochemical staining. Moreover, univariate and multivariate analyses were performed to determine the correlations between RBMS3 expression, MVD and patient prognosis. Cellular functions including proliferation, clonogenicity and apoptosis, were assessed to further identify in vitro roles of RBMS3. It was revealed that both mRNA and protein expression levels of RBMS3 were significantly lower in GBC tissues than in normal controls. Multivariate Cox regression analyses demonstrated cytoplasmic RBMS3 expression as an independent prognostic factor correlated with GBC angiogenesis, histopathological differentiation and TNM stage. Kaplan‑Meier curves revealed that patients with lower cytoplasmic RBMS3 levels had a significantly worse OS than patients with higher cytoplasmic RBMS3 expression. Additionally, ectopic expression of RBMS3 markedly suppressed GBC cell proliferation and clonogenicity and promoted apoptosis in vitro. These findings indicated the potential of cytoplasmic RBMS3 as a tumor prognostic biomarker and a promising therapeutic target for GBC.

Authors+Show Affiliations

Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.Department of General Surgery, The Armed Police Corps Hospital of Anhui, Hefei, Anhui 230041, P.R. China.Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.Department of General Surgery, The Armed Police Corps Hospital of Anhui, Hefei, Anhui 230041, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32627033

Citation

Wu, Youliang, et al. "Increased Expression of RBMS3 Predicts a Favorable Prognosis in Human Gallbladder Carcinoma." Oncology Reports, vol. 44, no. 1, 2020, pp. 55-68.
Wu Y, Meng D, You Y, et al. Increased expression of RBMS3 predicts a favorable prognosis in human gallbladder carcinoma. Oncol Rep. 2020;44(1):55-68.
Wu, Y., Meng, D., You, Y., Sun, R., Yan, Q., Bao, J., Sun, Y., Yun, D., Li, Y., & Sun, D. (2020). Increased expression of RBMS3 predicts a favorable prognosis in human gallbladder carcinoma. Oncology Reports, 44(1), 55-68. https://doi.org/10.3892/or.2020.7594
Wu Y, et al. Increased Expression of RBMS3 Predicts a Favorable Prognosis in Human Gallbladder Carcinoma. Oncol Rep. 2020;44(1):55-68. PubMed PMID: 32627033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased expression of RBMS3 predicts a favorable prognosis in human gallbladder carcinoma. AU - Wu,Youliang, AU - Meng,Delong, AU - You,Yexiang, AU - Sun,Ruochuan, AU - Yan,Qiang, AU - Bao,Junjun, AU - Sun,Yanjun, AU - Yun,Dapeng, AU - Li,Yongxiang, AU - Sun,Dengqun, Y1 - 2020/04/23/ PY - 2019/10/27/received PY - 2020/03/22/accepted PY - 2020/7/7/entrez PY - 2020/7/7/pubmed PY - 2020/7/7/medline SP - 55 EP - 68 JF - Oncology reports JO - Oncol. Rep. VL - 44 IS - 1 N2 - Multiple regions in the short arm of chromosome 3 are frequently deleted in a variety of solid tumors including gallbladder carcinoma (GBC). RNA binding motif, single‑stranded interacting protein 3 (RBMS3), a tumor suppressor gene (TSG), is located in this region. However, the role of RBMS3 in GBC remains unclear. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting were performed to evaluate the mRNA and protein expression levels of RBMS3 in 41 fresh frozen GBC tissues and paired normal tissues. An immunohistochemical assay was performed on a tissue microarray (TMA, consisting of 125 cases GBC and 47 normal controls). Microvessel density (MVD) counts were determined using CD34 immunohistochemical staining. Moreover, univariate and multivariate analyses were performed to determine the correlations between RBMS3 expression, MVD and patient prognosis. Cellular functions including proliferation, clonogenicity and apoptosis, were assessed to further identify in vitro roles of RBMS3. It was revealed that both mRNA and protein expression levels of RBMS3 were significantly lower in GBC tissues than in normal controls. Multivariate Cox regression analyses demonstrated cytoplasmic RBMS3 expression as an independent prognostic factor correlated with GBC angiogenesis, histopathological differentiation and TNM stage. Kaplan‑Meier curves revealed that patients with lower cytoplasmic RBMS3 levels had a significantly worse OS than patients with higher cytoplasmic RBMS3 expression. Additionally, ectopic expression of RBMS3 markedly suppressed GBC cell proliferation and clonogenicity and promoted apoptosis in vitro. These findings indicated the potential of cytoplasmic RBMS3 as a tumor prognostic biomarker and a promising therapeutic target for GBC. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/32627033/Increased_expression_of_RBMS3_predicts_a_favorable_prognosis_in_human_gallbladder_carcinoma L2 - http://www.spandidos-publications.com/or/44/1/55 DB - PRIME DP - Unbound Medicine ER -
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