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Anti-nociceptive effect of Euphorbia hirta leaf extract: Involvement of adenosine, cholinergic, and opioid receptors.
Cent Nerv Syst Agents Med Chem. 2020 07 05 [Online ahead of print]CN

Abstract

OBJECTIVE

The study was designed to investigate the anti-nociceptive activity of Euphorbia hirta leaf and its possible mechanism of action.

METHODS

The extract of Euphorbia hirta obtained from the leaf was prepared as per standard procedures and evaluated at the three doses (300, 600, and 1200 mg/kg, i.p). The extract was screened for anti-nociceptive activity using heat-induced (tail-flick) and chemical-induced (acetic acid-induced writhing and formalin-induced paw lick) nociception models in mice. The possible mechanism of action of the extract was evaluated using antagonists of notable nociceptive pathways.

RESULTS

Intraperitoneal administration of Euphorbia hirta extract at the doses of 600 and 1200 mg/kg significantly (p < 0.05) reduced the formalin-induced paw licking in both neurogenic and inflammatory phases of the test. While administration of the extract at the dose of 300 mg/kg significantly inhibited the pain due to formalin in the inflammatory phase but not in the neurogenic phase. The anti-nociceptive effect of Euphorbia hirta extract increased the reaction time to thermal stimulus, also inhibited the acetic acid-induced writhing dose-dependently. The anti-nociceptive effect exhibited by Euphorbia hirta extract in the formalin test was reversed by the administration of naloxone, theophylline, and atropine. Glibenclamide, nifedipine, and yohimbine, however, did not significantly block the anti-nociceptive effect of the extract. Meanwhile, methylene blue administration enhanced the anti-nociceptive effect of the extract.

CONCLUSION

The results indicated that Euphorbia hirta extract produces a dose-related anti-nociceptive effect in several models of chemical and thermal pain, through mechanisms that might involve interaction with adenosine, cholinergic, and opioid receptors.

Authors+Show Affiliations

Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Health Sciences, Osogbo. Ladoke Akintola University of Technology Ogbomoso. Nigeria.Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Health Sciences, Osogbo. Ladoke Akintola University of Technology Ogbomoso. Nigeria.Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Health Sciences, Osogbo. Ladoke Akintola University of Technology Ogbomoso. Nigeria.Department of Microbiology and Parasitology, Ladoke Akintola University of Technology Ogbomoso. Nigeria.Department of Microbiology and Parasitology, Ladoke Akintola University of Technology Ogbomoso. Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32628599

Citation

Olatoyan-Layonu, T J., et al. "Anti-nociceptive Effect of Euphorbia Hirta Leaf Extract: Involvement of Adenosine, Cholinergic, and Opioid Receptors." Central Nervous System Agents in Medicinal Chemistry, 2020.
Olatoyan-Layonu TJ, Wakeel OK, Abe AI, et al. Anti-nociceptive effect of Euphorbia hirta leaf extract: Involvement of adenosine, cholinergic, and opioid receptors. Cent Nerv Syst Agents Med Chem. 2020.
Olatoyan-Layonu, T. J., Wakeel, O. K., Abe, A. I., Ojurongbe, O., & Adeyeba, O. A. (2020). Anti-nociceptive effect of Euphorbia hirta leaf extract: Involvement of adenosine, cholinergic, and opioid receptors. Central Nervous System Agents in Medicinal Chemistry. https://doi.org/10.2174/1871524920666200705221956
Olatoyan-Layonu TJ, et al. Anti-nociceptive Effect of Euphorbia Hirta Leaf Extract: Involvement of Adenosine, Cholinergic, and Opioid Receptors. Cent Nerv Syst Agents Med Chem. 2020 07 5; PubMed PMID: 32628599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-nociceptive effect of Euphorbia hirta leaf extract: Involvement of adenosine, cholinergic, and opioid receptors. AU - Olatoyan-Layonu,T J, AU - Wakeel,O K, AU - Abe,A I, AU - Ojurongbe,Olusola, AU - Adeyeba,O A, Y1 - 2020/07/05/ PY - 2020/03/05/received PY - 2020/06/08/revised PY - 2020/06/16/accepted PY - 2020/7/7/entrez PY - 2020/7/7/pubmed PY - 2020/7/7/medline KW - Euphorbia hirta KW - adenosine KW - and opioid. KW - anti-nociceptive KW - cholinergic KW - formalin JF - Central nervous system agents in medicinal chemistry JO - Cent Nerv Syst Agents Med Chem N2 - OBJECTIVE: The study was designed to investigate the anti-nociceptive activity of Euphorbia hirta leaf and its possible mechanism of action. METHODS: The extract of Euphorbia hirta obtained from the leaf was prepared as per standard procedures and evaluated at the three doses (300, 600, and 1200 mg/kg, i.p). The extract was screened for anti-nociceptive activity using heat-induced (tail-flick) and chemical-induced (acetic acid-induced writhing and formalin-induced paw lick) nociception models in mice. The possible mechanism of action of the extract was evaluated using antagonists of notable nociceptive pathways. RESULTS: Intraperitoneal administration of Euphorbia hirta extract at the doses of 600 and 1200 mg/kg significantly (p < 0.05) reduced the formalin-induced paw licking in both neurogenic and inflammatory phases of the test. While administration of the extract at the dose of 300 mg/kg significantly inhibited the pain due to formalin in the inflammatory phase but not in the neurogenic phase. The anti-nociceptive effect of Euphorbia hirta extract increased the reaction time to thermal stimulus, also inhibited the acetic acid-induced writhing dose-dependently. The anti-nociceptive effect exhibited by Euphorbia hirta extract in the formalin test was reversed by the administration of naloxone, theophylline, and atropine. Glibenclamide, nifedipine, and yohimbine, however, did not significantly block the anti-nociceptive effect of the extract. Meanwhile, methylene blue administration enhanced the anti-nociceptive effect of the extract. CONCLUSION: The results indicated that Euphorbia hirta extract produces a dose-related anti-nociceptive effect in several models of chemical and thermal pain, through mechanisms that might involve interaction with adenosine, cholinergic, and opioid receptors. SN - 1875-6166 UR - https://www.unboundmedicine.com/medline/citation/32628599/Anti-nociceptive_effect_of_Euphorbia_hirta_leaf_extract:_Involvement_of_adenosine,_cholinergic,_and_opioid_receptors L2 - https://www.eurekaselect.com/183488/article DB - PRIME DP - Unbound Medicine ER -
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