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Comprehensive two-dimensional liquid chromatography-based quali-quantitative screening of aqueous phases from pyrolysis bio-oils.
Electrophoresis. 2020 Jul 06 [Online ahead of print]E

Abstract

Pyrolysis processes are an alternative to minimize the environmental problem associated to agrifood industrial wastes. The main product resulting from these processes is a high-value liquid product, called bio-oil. Recently, the use of comprehensive two-dimensional liquid chromatography (LC × LC) has been demonstrated as a useful tool to improve the characterization of the water-soluble phases of bio-oils, considering their complexity and high water content. However, the precise composition of bio-oils from different agrifood byproducts is still unknown. In the present study, the qualitative and quantitative screening of eight aqueous phases from different biomasses, not yet reported in the literature, using LC × LC is presented. The two-dimensional approach was based on the use of two reverse phase separations. An amide column in the first dimension together with a C18 column in the second dimension were employed. Thanks to the use of diode array and mass spectrometry detection, 28 compounds were identified and quantified in the aqueous phase samples with good figures of merit. Samples showed a distinct quali-quantitative composition and a great predominance of compounds belonging to aldehydes, ketones and phenols, most of them with high polarity.

Authors+Show Affiliations

Institute of Chemistry, Porto Alegre, Rio Grande do Sul, Brazil.Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina, Italy.Institute of Chemistry, Porto Alegre, Rio Grande do Sul, Brazil. Department of Industrial Biotechnology, Tiradentes University, Sergipe, Brazil.Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina, Italy. Unit of Food Science and Nutrition, Department of Medicine, University Campus Bio-Medico, Rome, Italy. Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina, Italy. Unit of Food Science and Nutrition, Department of Medicine, University Campus Bio-Medico, Rome, Italy. Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC-UAM), Madrid, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32628775

Citation

Lazzari, Eliane, et al. "Comprehensive Two-dimensional Liquid Chromatography-based Quali-quantitative Screening of Aqueous Phases From Pyrolysis Bio-oils." Electrophoresis, 2020.
Lazzari E, Arena K, Caramão EB, et al. Comprehensive two-dimensional liquid chromatography-based quali-quantitative screening of aqueous phases from pyrolysis bio-oils. Electrophoresis. 2020.
Lazzari, E., Arena, K., Caramão, E. B., Dugo, P., Mondello, L., & Herrero, M. (2020). Comprehensive two-dimensional liquid chromatography-based quali-quantitative screening of aqueous phases from pyrolysis bio-oils. Electrophoresis. https://doi.org/10.1002/elps.202000119
Lazzari E, et al. Comprehensive Two-dimensional Liquid Chromatography-based Quali-quantitative Screening of Aqueous Phases From Pyrolysis Bio-oils. Electrophoresis. 2020 Jul 6; PubMed PMID: 32628775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive two-dimensional liquid chromatography-based quali-quantitative screening of aqueous phases from pyrolysis bio-oils. AU - Lazzari,Eliane, AU - Arena,Katia, AU - Caramão,Elina B, AU - Dugo,Paola, AU - Mondello,Luigi, AU - Herrero,Miguel, Y1 - 2020/07/06/ PY - 2020/05/08/received PY - 2020/06/23/revised PY - 2020/06/25/accepted PY - 2020/7/7/pubmed PY - 2020/7/7/medline PY - 2020/7/7/entrez KW - Aqueous phase KW - Bio-oil KW - LC × LC-DAD-ESI-MS KW - Pyrolysis KW - Quantification JF - Electrophoresis JO - Electrophoresis N2 - Pyrolysis processes are an alternative to minimize the environmental problem associated to agrifood industrial wastes. The main product resulting from these processes is a high-value liquid product, called bio-oil. Recently, the use of comprehensive two-dimensional liquid chromatography (LC × LC) has been demonstrated as a useful tool to improve the characterization of the water-soluble phases of bio-oils, considering their complexity and high water content. However, the precise composition of bio-oils from different agrifood byproducts is still unknown. In the present study, the qualitative and quantitative screening of eight aqueous phases from different biomasses, not yet reported in the literature, using LC × LC is presented. The two-dimensional approach was based on the use of two reverse phase separations. An amide column in the first dimension together with a C18 column in the second dimension were employed. Thanks to the use of diode array and mass spectrometry detection, 28 compounds were identified and quantified in the aqueous phase samples with good figures of merit. Samples showed a distinct quali-quantitative composition and a great predominance of compounds belonging to aldehydes, ketones and phenols, most of them with high polarity. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/32628775/Comprehensive_two-dimensional_liquid_chromatography-based_quali-quantitative_screening_of_aqueous_phases_from_pyrolysis_bio-oils L2 - https://doi.org/10.1002/elps.202000119 DB - PRIME DP - Unbound Medicine ER -
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