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Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis.
J Proteomics. 2020 Jul 04; 226:103891.JP

Abstract

Lysine crotonylation (Kcr) is a recently-discovered type of post-translational modification. Although Kcr has been reported in many species, little is known about this process in crustaceans. In this study, pan anti-lysine crotonylation antibody enrichment and high-resolution liquid chromatogram-mass spectrometry analysis were employed to characterize Kcr in testis of the Chinese mitten crab Eriocheir sinensis testis. Overall, 2799 Kcr sites were identified on 908 proteins with 14 conserved motifs. Bioinformatics analysis showed that Kcr was predominant on proteins found in cytoplasm, mitochondria and nucleus, and those involved in ribosome, proteasome, carbon metabolism and protein processing in endoplasmic reticulum. In total, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. These differentially expressed proteins were enriched in protein processing in endoplasmic reticulum pathway during formation of acrosome. In contrast, histone Kcr associated with mammalian spermatogenesis. These results provide foundational knowledge on the role of non-histone Kcr in spermiogenesis of E. sinensis.

SIGNIFICANCE:

Lysine crotonylation (Kcr) is a recently-identified post-translational modification, and histone Kcr was found to associate with mammalian spermatogenesis. However, crotonylation of non-histone proteins has not been reported in spermatogenesis regulation. Further, there is no information on crotonylation in crustaceans. This study was the first large-scale Kcr proteome characterization in crustaceans. A total of 2799 Kcr sites on 908 proteins with 14 conserved motifs were identified from Eriocheir sinensis testis. Of which, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. Our results provide the basic information for further functional validation of Kcr proteins and revealed new roles of Kcr in spermiogenesis of E. sinensis.

Authors+Show Affiliations

School of Marine Science, Ningbo University, Ningbo 315020, China.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.School of Marine Science, Ningbo University, Ningbo 315020, China.School of Marine Science, Ningbo University, Ningbo 315020, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: cuizhaoxia@nbu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32629196

Citation

Bao, Chenchang, et al. "Large-scale Lysine Crotonylation Analysis Reveals Its Potential Role in Spermiogenesis in the Chinese Mitten Crab Eriocheir Sinensis." Journal of Proteomics, vol. 226, 2020, p. 103891.
Bao C, Song C, Liu Y, et al. Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis. J Proteomics. 2020;226:103891.
Bao, C., Song, C., Liu, Y., Yang, Y., & Cui, Z. (2020). Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis. Journal of Proteomics, 226, 103891. https://doi.org/10.1016/j.jprot.2020.103891
Bao C, et al. Large-scale Lysine Crotonylation Analysis Reveals Its Potential Role in Spermiogenesis in the Chinese Mitten Crab Eriocheir Sinensis. J Proteomics. 2020 Jul 4;226:103891. PubMed PMID: 32629196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis. AU - Bao,Chenchang, AU - Song,Chengwen, AU - Liu,Yuan, AU - Yang,Yanan, AU - Cui,Zhaoxia, Y1 - 2020/07/04/ PY - 2019/12/09/received PY - 2020/03/15/revised PY - 2020/06/26/accepted PY - 2020/7/7/pubmed PY - 2020/7/7/medline PY - 2020/7/7/entrez KW - Eriocheir sinensis KW - Lysine crotonylation KW - Proteomics KW - Spermiogenesis SP - 103891 EP - 103891 JF - Journal of proteomics JO - J Proteomics VL - 226 N2 - Lysine crotonylation (Kcr) is a recently-discovered type of post-translational modification. Although Kcr has been reported in many species, little is known about this process in crustaceans. In this study, pan anti-lysine crotonylation antibody enrichment and high-resolution liquid chromatogram-mass spectrometry analysis were employed to characterize Kcr in testis of the Chinese mitten crab Eriocheir sinensis testis. Overall, 2799 Kcr sites were identified on 908 proteins with 14 conserved motifs. Bioinformatics analysis showed that Kcr was predominant on proteins found in cytoplasm, mitochondria and nucleus, and those involved in ribosome, proteasome, carbon metabolism and protein processing in endoplasmic reticulum. In total, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. These differentially expressed proteins were enriched in protein processing in endoplasmic reticulum pathway during formation of acrosome. In contrast, histone Kcr associated with mammalian spermatogenesis. These results provide foundational knowledge on the role of non-histone Kcr in spermiogenesis of E. sinensis. SIGNIFICANCE: Lysine crotonylation (Kcr) is a recently-identified post-translational modification, and histone Kcr was found to associate with mammalian spermatogenesis. However, crotonylation of non-histone proteins has not been reported in spermatogenesis regulation. Further, there is no information on crotonylation in crustaceans. This study was the first large-scale Kcr proteome characterization in crustaceans. A total of 2799 Kcr sites on 908 proteins with 14 conserved motifs were identified from Eriocheir sinensis testis. Of which, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. Our results provide the basic information for further functional validation of Kcr proteins and revealed new roles of Kcr in spermiogenesis of E. sinensis. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/32629196/Large-scale_lysine_crotonylation_analysis_reveals_its_potential_role_in_spermiogenesis_in_the_Chinese_mitten_crab_Eriocheir_sinensis L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(20)30259-1 DB - PRIME DP - Unbound Medicine ER -
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