Tags

Type your tag names separated by a space and hit enter

Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples from Local Markets in Northern Thailand by Developed Immunoassay.
Int J Environ Res Public Health. 2020 06 30; 17(13)IJ

Abstract

Chlorpyrifos is an organophosphate pesticide that is wildly used among farmers for crop protection. However, there are concerns regarding its contamination in the environment and food chain. In the present study, an in-house indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) specific for detecting chlorpyrifos is developed and validated against gas chromatography-flame photometric detection (GC-FPD) as the conventional method. The developed ic-ELISA was used for detecting chlorpyrifos residue in vegetable samples. The developed ic-ELISA showed good sensitivity to chlorpyrifos at an IC50 of 0.80 µg/kg, with low cross-reactivity to other organophosphate pesticides. The 160 samples were collected from local markets located in the Chiang Rai, Chiang Mai, and Nan provinces in northern Thailand. The positive rate of chlorpyrifos residues in the vegetable samples was 33.8%, with the highest levels found in cucumbers, coriander, and morning glory, at 275, 145, and 35.3 µg/kg, respectively. The highest median levels of chlorpyrifos found in the detected samples were Chinese cabbage (332 μg/kg), cucumber (146.3 μg/kg) and Chinese Kale (26.95 μg/kg). The developed ic-ELISA is suitable for the rapid quantitation of chlorpyrifos residues.

Links

PMC Free PDF

Authors+Show Affiliations

Hongsibsong S
Research Institute for Health Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Prapamontol T
Research Institute for Health Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Xu T
Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Hammock BD
Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Wang H
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Chen ZJ
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Xu ZL
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

MeSH

ChlorpyrifosFood ContaminationInsecticidesPesticide ResiduesThailandVegetables

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32630084

Citation

Hongsibsong, Surat, et al. "Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples From Local Markets in Northern Thailand By Developed Immunoassay." International Journal of Environmental Research and Public Health, vol. 17, no. 13, 2020.
Hongsibsong S, Prapamontol T, Xu T, et al. Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples from Local Markets in Northern Thailand by Developed Immunoassay. Int J Environ Res Public Health. 2020;17(13).
Hongsibsong, S., Prapamontol, T., Xu, T., Hammock, B. D., Wang, H., Chen, Z. J., & Xu, Z. L. (2020). Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples from Local Markets in Northern Thailand by Developed Immunoassay. International Journal of Environmental Research and Public Health, 17(13). https://doi.org/10.3390/ijerph17134723
Hongsibsong S, et al. Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples From Local Markets in Northern Thailand By Developed Immunoassay. Int J Environ Res Public Health. 2020 06 30;17(13) PubMed PMID: 32630084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples from Local Markets in Northern Thailand by Developed Immunoassay. AU - Hongsibsong,Surat, AU - Prapamontol,Tippawan, AU - Xu,Ting, AU - Hammock,Bruce D, AU - Wang,Hong, AU - Chen,Zi-Jian, AU - Xu,Zhen-Lin, Y1 - 2020/06/30/ PY - 2020/05/16/received PY - 2020/06/24/revised PY - 2020/06/25/accepted PY - 2020/7/8/entrez PY - 2020/7/8/pubmed PY - 2020/11/24/medline KW - GC-FPD KW - chlorpyrifos residue KW - ic-ELISA KW - northern Thailand JF - International journal of environmental research and public health JO - Int J Environ Res Public Health VL - 17 IS - 13 N2 - Chlorpyrifos is an organophosphate pesticide that is wildly used among farmers for crop protection. However, there are concerns regarding its contamination in the environment and food chain. In the present study, an in-house indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) specific for detecting chlorpyrifos is developed and validated against gas chromatography-flame photometric detection (GC-FPD) as the conventional method. The developed ic-ELISA was used for detecting chlorpyrifos residue in vegetable samples. The developed ic-ELISA showed good sensitivity to chlorpyrifos at an IC50 of 0.80 µg/kg, with low cross-reactivity to other organophosphate pesticides. The 160 samples were collected from local markets located in the Chiang Rai, Chiang Mai, and Nan provinces in northern Thailand. The positive rate of chlorpyrifos residues in the vegetable samples was 33.8%, with the highest levels found in cucumbers, coriander, and morning glory, at 275, 145, and 35.3 µg/kg, respectively. The highest median levels of chlorpyrifos found in the detected samples were Chinese cabbage (332 μg/kg), cucumber (146.3 μg/kg) and Chinese Kale (26.95 μg/kg). The developed ic-ELISA is suitable for the rapid quantitation of chlorpyrifos residues. SN - 1660-4601 UR - https://www.unboundmedicine.com/medline/citation/32630084/Monitoring_of_the_Organophosphate_Pesticide_Chlorpyrifos_in_Vegetable_Samples_from_Local_Markets_in_Northern_Thailand_by_Developed_Immunoassay_ DB - PRIME DP - Unbound Medicine ER -