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Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway.
Mar Drugs. 2020 Jun 25; 18(6)MD

Abstract

The main pathologic changes of the Parkinson's disease (PD) is dopaminergic (DA) neurons lost. Apoptosis was one of the important reasons involved in the DA lost. Our previous study found a fucoidan fraction sulfated heterosaccharide (UF) had neuroprotective activity. The aim of this study was to clarify the mechanism of UF on DA neurons using human dopaminergic neuroblastoma (SH-SY5Y) cells a typical as a PD cellular model. Results showed that UF prevented MPP+-induced SH-SY5Y cells apoptosis and cell death. Additionally, UF pretreated cells increased phosphorylation of Akt, PI3K and NGF, which means UF-treated active PI3K-Akt pathway. Moreover, UF treated cells decreased the expression of apoptosis-associated protein, such as the ratio of Bax/Bcl-2, GSK3β, caspase-3 and p53 nuclear induced by MPP+. This effect was partially blocked by PI3K inhibitor LY294002. Our data suggested that protective effect of UF against MPP+-induced SH-SY5Y cells death by affecting the PI3K-Akt pathway. These findings contribute to a better understanding of the critical roles of UF in treating PD and may elucidate the molecular mechanisms of UF effects in PD.

Authors+Show Affiliations

School of Life Sciences, Nantong University, Seyuan Road 9, Nantong 226019, China.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Wenhai Road, Aoshanwei, Jimo, Qingdao 266237, China.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Wenhai Road, Aoshanwei, Jimo, Qingdao 266237, China.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Wenhai Road, Aoshanwei, Jimo, Qingdao 266237, China.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Wenhai Road, Aoshanwei, Jimo, Qingdao 266237, China.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Wenhai Road, Aoshanwei, Jimo, Qingdao 266237, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32630523

Citation

Liu, Huaide, et al. "Protective Effect of Fucoidan Against MPP+-Induced SH-SY5Y Cells Apoptosis By Affecting the PI3K/Akt Pathway." Marine Drugs, vol. 18, no. 6, 2020.
Liu H, Wang J, Zhang Q, et al. Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway. Mar Drugs. 2020;18(6).
Liu, H., Wang, J., Zhang, Q., Geng, L., Yang, Y., & Wu, N. (2020). Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway. Marine Drugs, 18(6). https://doi.org/10.3390/md18060333
Liu H, et al. Protective Effect of Fucoidan Against MPP+-Induced SH-SY5Y Cells Apoptosis By Affecting the PI3K/Akt Pathway. Mar Drugs. 2020 Jun 25;18(6) PubMed PMID: 32630523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway. AU - Liu,Huaide, AU - Wang,Jing, AU - Zhang,Quanbin, AU - Geng,Lihua, AU - Yang,Yue, AU - Wu,Ning, Y1 - 2020/06/25/ PY - 2020/05/09/received PY - 2020/06/08/revised PY - 2020/06/23/accepted PY - 2020/7/8/entrez PY - 2020/7/8/pubmed PY - 2021/4/28/medline KW - PI3K–Akt KW - dopamine neurons apoptosis KW - fucoidan KW - sulfated heterosaccharide JF - Marine drugs JO - Mar Drugs VL - 18 IS - 6 N2 - The main pathologic changes of the Parkinson's disease (PD) is dopaminergic (DA) neurons lost. Apoptosis was one of the important reasons involved in the DA lost. Our previous study found a fucoidan fraction sulfated heterosaccharide (UF) had neuroprotective activity. The aim of this study was to clarify the mechanism of UF on DA neurons using human dopaminergic neuroblastoma (SH-SY5Y) cells a typical as a PD cellular model. Results showed that UF prevented MPP+-induced SH-SY5Y cells apoptosis and cell death. Additionally, UF pretreated cells increased phosphorylation of Akt, PI3K and NGF, which means UF-treated active PI3K-Akt pathway. Moreover, UF treated cells decreased the expression of apoptosis-associated protein, such as the ratio of Bax/Bcl-2, GSK3β, caspase-3 and p53 nuclear induced by MPP+. This effect was partially blocked by PI3K inhibitor LY294002. Our data suggested that protective effect of UF against MPP+-induced SH-SY5Y cells death by affecting the PI3K-Akt pathway. These findings contribute to a better understanding of the critical roles of UF in treating PD and may elucidate the molecular mechanisms of UF effects in PD. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/32630523/Protective_Effect_of_Fucoidan_against_MPP+_Induced_SH_SY5Y_Cells_Apoptosis_by_Affecting_the_PI3K/Akt_Pathway_ L2 - https://www.mdpi.com/resolver?pii=md18060333 DB - PRIME DP - Unbound Medicine ER -