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[New immunohistochemistry markers to determine presence of vas deferens in vasectomy specimen.]
Arch Esp Urol. 2020 07; 73(6):534-540.AE

Abstract

OBJECTIVES

The pathological examination of vasectomy specimens to confirm the presence of vas deferens is usually simple and is done by routine hematoxylinand eosin staining. Use of immunohistochemical techniques can aid to the diagnosis in those cases with artifacts of the epithelium, and they are also useful to differentiate vas deferens from blood vessel. We have investigated the usefulness of CD31, CD34, ERG and PAX8 for these purposes.

MATERIAL AND METHODS

81 sections from vasectomy specimens in which any section showed some kind of epithelial artifact were analyzed. Immunohistochemistry was performed with monoclonal antibodies for CD31 (clone JC70), CD34 (clone QBEnd/10), ERG (clone EPR3864) and PAX8 (clone MRQ-50). Evaluation of the vas deferens and vascular endothelial staining was done.

RESULTS

Histologically, vas deferens epithelium was well-preserved in 18 sections (22.2%), denuded in 6 (7.4%), crushed or distorted in 48 sections (59.3%), detached in 5 (6,2%), and misplaced out of the vas deferens lumen in 4 (4.9%). In most of the sections the epithelium showed weak (86.4%) or moderate (9.9%) CD31 cytoplasmic staining, as well as strong nuclear PAX8 reactivity in all of the sections, exhibiting a granular pattern in the detached or artifacted epithelium. CD34 and ERG were negative in the epithelium. Capillary vessel endothelium in the vas deferens wall showed strong cytoplasmic positivity for CD31 and CD34, as well as nuclear ERG reactivity, being PAX8 negative.

CONCLUSIONS

PAX8 is a useful antibody to confirm the presence of vas deferens in artifacted vasectomy specimens. CD34 and ERG are negative in the epithelium,and, otherwise, they are expressed by vascular endothelium, with the advantage of nuclear staining pattern for ERG. CD31, a classic endothelial marker, is not so specific as it had been stated as it shows weak or moderate expression in the vas deferens epithelium.

Authors+Show Affiliations

Grupo de Investigación en Uropatología. Instituto de Investigación Sanitaria Galicia Sur. SERGAS-UVIGO. Vigo. Pontevedra. España. Servicio de Anatomía Patológica. Complexo Hospitalario Universitario de Vigo. Vigo. Pontevedra. España.Servicio de Anatomía Patológica. Complexo Hospitalario Universitario de Vigo. Vigo. Pontevedra. España.Servicio de Urología. Complexo Hospitalario Universitario de Vigo. Vigo. Pontevedra. España.Grupo de Investigación en Uropatología. Instituto de Investigación Sanitaria Galicia Sur. SERGAS-UVIGO. Vigo. Pontevedra. España. Servicio de Anatomía Patológica. Complexo Hospitalario Universitario de Vigo. Vigo. Pontevedra. España.Servicio de Anatomía Patológica. Hospital POVISA. Vigo. Pontevedra. España.

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

32633248

Citation

Ortiz-Rey, José Antonio, et al. "[New Immunohistochemistry Markers to Determine Presence of Vas Deferens in Vasectomy Specimen.]." Archivos Espanoles De Urologia, vol. 73, no. 6, 2020, pp. 534-540.
Ortiz-Rey JA, Domínguez-de Dios J, Pérez-Schoch M, et al. [New immunohistochemistry markers to determine presence of vas deferens in vasectomy specimen.]. Arch Esp Urol. 2020;73(6):534-540.
Ortiz-Rey, J. A., Domínguez-de Dios, J., Pérez-Schoch, M., San Miguel-Fraile, P., & Gómez-de María, C. (2020). [New immunohistochemistry markers to determine presence of vas deferens in vasectomy specimen.]. Archivos Espanoles De Urologia, 73(6), 534-540.
Ortiz-Rey JA, et al. [New Immunohistochemistry Markers to Determine Presence of Vas Deferens in Vasectomy Specimen.]. Arch Esp Urol. 2020;73(6):534-540. PubMed PMID: 32633248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [New immunohistochemistry markers to determine presence of vas deferens in vasectomy specimen.] AU - Ortiz-Rey,José Antonio, AU - Domínguez-de Dios,Julia, AU - Pérez-Schoch,Miguel, AU - San Miguel-Fraile,Pilar, AU - Gómez-de María,Carolina, PY - 2020/7/8/entrez PY - 2020/7/8/pubmed PY - 2020/7/8/medline KW - Conducto deferente KW - ERG KW - Immunohistochemistry KW - Inmunohistoquímica KW - PAX8 KW - Vas deferens KW - Vasectomy KW - Vasectomía SP - 534 EP - 540 JF - Archivos espanoles de urologia JO - Arch. Esp. Urol. VL - 73 IS - 6 N2 - OBJECTIVES: The pathological examination of vasectomy specimens to confirm the presence of vas deferens is usually simple and is done by routine hematoxylinand eosin staining. Use of immunohistochemical techniques can aid to the diagnosis in those cases with artifacts of the epithelium, and they are also useful to differentiate vas deferens from blood vessel. We have investigated the usefulness of CD31, CD34, ERG and PAX8 for these purposes. MATERIAL AND METHODS: 81 sections from vasectomy specimens in which any section showed some kind of epithelial artifact were analyzed. Immunohistochemistry was performed with monoclonal antibodies for CD31 (clone JC70), CD34 (clone QBEnd/10), ERG (clone EPR3864) and PAX8 (clone MRQ-50). Evaluation of the vas deferens and vascular endothelial staining was done. RESULTS: Histologically, vas deferens epithelium was well-preserved in 18 sections (22.2%), denuded in 6 (7.4%), crushed or distorted in 48 sections (59.3%), detached in 5 (6,2%), and misplaced out of the vas deferens lumen in 4 (4.9%). In most of the sections the epithelium showed weak (86.4%) or moderate (9.9%) CD31 cytoplasmic staining, as well as strong nuclear PAX8 reactivity in all of the sections, exhibiting a granular pattern in the detached or artifacted epithelium. CD34 and ERG were negative in the epithelium. Capillary vessel endothelium in the vas deferens wall showed strong cytoplasmic positivity for CD31 and CD34, as well as nuclear ERG reactivity, being PAX8 negative. CONCLUSIONS: PAX8 is a useful antibody to confirm the presence of vas deferens in artifacted vasectomy specimens. CD34 and ERG are negative in the epithelium,and, otherwise, they are expressed by vascular endothelium, with the advantage of nuclear staining pattern for ERG. CD31, a classic endothelial marker, is not so specific as it had been stated as it shows weak or moderate expression in the vas deferens epithelium. SN - 0004-0614 UR - https://www.unboundmedicine.com/medline/citation/32633248/[New_immunohistochemistry_markers_to_determine_presence_of_vas_deferens_in_vasectomy_specimen.] L2 - http://www.aeurologia.com/article_detail.php?aid=ad7b6999ebcf3c025668dde834cc8c5e9d6fb603 DB - PRIME DP - Unbound Medicine ER -
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