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Immature, double negative (CD4-,CD8-) rat thymocytes do not express IL-2 receptors.
J Immunol. 1988 Dec 01; 141(11):3810-8.JI

Abstract

IL-2R alpha-chain is expressed on a subset of mouse CD4- and CD8-, double negative (DN) thymocytes. This expression of IL-2R alpha-chain on some DN thymocytes in the mouse has led to the proposal that IL-2 might serve as a principal growth and/or differentiation factor for immature thymocytes. However, previous histologic observations have indicated that IL-2R alpha-chain is not expressed on the subcapsular thymic blasts (an area rich in DN cells) in either huma or rat thymus, whereas all three species display IL-2R expression on a few cells in the thymic medulla. Therefore, we characterized rat DN thymocytes to determine whether they contained an IL-2R+ population. The results show that rat thymic DN cells share several characteristics with mouse DN cells. However, most of the rat strains do not express the IL-2R on DN cells as shown either by immunofluorescence or by IL-2 binding and receptor cross-linking. Thus, the rare medullary IL-2R+ cells were not found in the DN cells. Only in the exceptional F344 rat strain is the IL-2R alpha-chain expressed on a major proportion of thymocytes, including both DN cells and small cortical-type thymocytes. Furthermore, rat DN cells do not contain detectable IL-2 mRNA or cytoplasmic IL-2 activity, thus supporting the conclusion that it is unlikely that IL-2 and IL-2R serve to maintain the proliferation of rat DN thymocytes in vivo. The possible significance of in vivo expression of IL-2R alpha-chain on immature thymocytes in the mouse and in a single rat strain is discussed.

Authors+Show Affiliations

Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701-1013.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3263434

Citation

Takács, L, et al. "Immature, Double Negative (CD4-,CD8-) Rat Thymocytes Do Not Express IL-2 Receptors." Journal of Immunology (Baltimore, Md. : 1950), vol. 141, no. 11, 1988, pp. 3810-8.
Takács L, Ruscetti FW, Kovacs EJ, et al. Immature, double negative (CD4-,CD8-) rat thymocytes do not express IL-2 receptors. J Immunol. 1988;141(11):3810-8.
Takács, L., Ruscetti, F. W., Kovacs, E. J., Rocha, B., Brocke, S., Diamantstein, T., & Mathieson, B. J. (1988). Immature, double negative (CD4-,CD8-) rat thymocytes do not express IL-2 receptors. Journal of Immunology (Baltimore, Md. : 1950), 141(11), 3810-8.
Takács L, et al. Immature, Double Negative (CD4-,CD8-) Rat Thymocytes Do Not Express IL-2 Receptors. J Immunol. 1988 Dec 1;141(11):3810-8. PubMed PMID: 3263434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immature, double negative (CD4-,CD8-) rat thymocytes do not express IL-2 receptors. AU - Takács,L, AU - Ruscetti,F W, AU - Kovacs,E J, AU - Rocha,B, AU - Brocke,S, AU - Diamantstein,T, AU - Mathieson,B J, PY - 1988/12/1/pubmed PY - 1988/12/1/medline PY - 1988/12/1/entrez SP - 3810 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 141 IS - 11 N2 - IL-2R alpha-chain is expressed on a subset of mouse CD4- and CD8-, double negative (DN) thymocytes. This expression of IL-2R alpha-chain on some DN thymocytes in the mouse has led to the proposal that IL-2 might serve as a principal growth and/or differentiation factor for immature thymocytes. However, previous histologic observations have indicated that IL-2R alpha-chain is not expressed on the subcapsular thymic blasts (an area rich in DN cells) in either huma or rat thymus, whereas all three species display IL-2R expression on a few cells in the thymic medulla. Therefore, we characterized rat DN thymocytes to determine whether they contained an IL-2R+ population. The results show that rat thymic DN cells share several characteristics with mouse DN cells. However, most of the rat strains do not express the IL-2R on DN cells as shown either by immunofluorescence or by IL-2 binding and receptor cross-linking. Thus, the rare medullary IL-2R+ cells were not found in the DN cells. Only in the exceptional F344 rat strain is the IL-2R alpha-chain expressed on a major proportion of thymocytes, including both DN cells and small cortical-type thymocytes. Furthermore, rat DN cells do not contain detectable IL-2 mRNA or cytoplasmic IL-2 activity, thus supporting the conclusion that it is unlikely that IL-2 and IL-2R serve to maintain the proliferation of rat DN thymocytes in vivo. The possible significance of in vivo expression of IL-2R alpha-chain on immature thymocytes in the mouse and in a single rat strain is discussed. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3263434/Immature_double_negative__CD4_CD8___rat_thymocytes_do_not_express_IL_2_receptors_ DB - PRIME DP - Unbound Medicine ER -