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Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom.
Toxicon. 2020 Oct 15; 185:26-35.T

Abstract

The South American rattlesnake Crotalus durissus spp has a wide geographic distribution in Brazil. Although responsible for only a low proportion of ophidian accidents, it is considered one of the most medically important species of venomous snakes due to the high mortality rate (1.87%). Snake venom is a complex phenotype commonly subjected to individual intraspecific, ontogenetic and geographic variability. Compositional differences in pooled venom used in the immunization process may impact the efficacy of the antivenom. In order to assure standardized high-quality antivenom, the potency of each Brazilian crotalic antivenom batch is determined against the 'Brazilian Crotalic Reference Venom' (BCRV). BCRV is produced by Instituto Butantan using venom obtained from the first milking of recently wild-caught C. d. terrificus specimens brought to the Institute. The decrease in the number of snake donations experienced in recent years can become a threat to the production of future batches of BCRV. To evaluate the feasibility of using venom from long-term captive animals in the formulation of BCRV, we have compared the proteomic, biochemical and biological profiles of C. d. terrificus venom pooled from captive specimens (CVP- captive venom pool) and BCRV. Electrophoretic and venomics analyses revealed a very similar venom composition profile, but also certain differences in toxins abundance, with some low abundant protein families found only in BCRV. Enzymatic (L-amino acid oxidase, phospholipase A2 and proteolytic) and biological (myotoxic and coagulant) activities showed higher values in CVP than in BCRV. CVP also possessed slightly higher lethal effect, although the Instituto Butantan crotalic antivenom showed equivalent potency neutralizing BCRV and CVP. Our results strongly suggest that venom from long-term captive C. d. terrificus might be a valid alternative to generate an immunization mixture of equivalent quality to the currently in use reference venom.

Authors+Show Affiliations

Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, 05503-000, Brazil.Laboratorio de Venómica Evolutiva y Traslacional, Instituto de Biomedicina, Consejo Superior de Investigaciones Científicas, Jaime Roig 11, 46010, Valencia, Spain.Laboratorio de Venómica Evolutiva y Traslacional, Instituto de Biomedicina, Consejo Superior de Investigaciones Científicas, Jaime Roig 11, 46010, Valencia, Spain. Electronic address: jcalvete@ibv.csic.es.Laboratório de Herpetologia, Instituto Butantan, São Paulo, 05503-000, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, 05503-000, Brazil. Electronic address: anita.azevedo@butantan.gov.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32634448

Citation

Tasima, Lidia J., et al. "Comparative Proteomic Profiling and Functional Characterization of Venom Pooled From Captive Crotalus Durissus Terrificus Specimens and the Brazilian Crotalic Reference Venom." Toxicon : Official Journal of the International Society On Toxinology, vol. 185, 2020, pp. 26-35.
Tasima LJ, Hatakeyama DM, Serino-Silva C, et al. Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom. Toxicon. 2020;185:26-35.
Tasima, L. J., Hatakeyama, D. M., Serino-Silva, C., Rodrigues, C. F. B., de Lima, E. O. V., Sant'Anna, S. S., Grego, K. F., de Morais-Zani, K., Sanz, L., Calvete, J. J., & Tanaka-Azevedo, A. M. (2020). Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom. Toxicon : Official Journal of the International Society On Toxinology, 185, 26-35. https://doi.org/10.1016/j.toxicon.2020.07.001
Tasima LJ, et al. Comparative Proteomic Profiling and Functional Characterization of Venom Pooled From Captive Crotalus Durissus Terrificus Specimens and the Brazilian Crotalic Reference Venom. Toxicon. 2020 Oct 15;185:26-35. PubMed PMID: 32634448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom. AU - Tasima,Lidia J, AU - Hatakeyama,Daniela M, AU - Serino-Silva,Caroline, AU - Rodrigues,Caroline F B, AU - de Lima,Eduardo O V, AU - Sant'Anna,Sávio S, AU - Grego,Kathleen F, AU - de Morais-Zani,Karen, AU - Sanz,Libia, AU - Calvete,Juan J, AU - Tanaka-Azevedo,Anita M, Y1 - 2020/07/04/ PY - 2020/05/19/received PY - 2020/06/26/revised PY - 2020/07/02/accepted PY - 2020/7/8/pubmed PY - 2020/9/10/medline PY - 2020/7/8/entrez KW - Brazilian crotalic reference venom KW - Crotalus durissus terrificus venom KW - Snake venomics KW - Venom of wild caught versus captive snake SP - 26 EP - 35 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 185 N2 - The South American rattlesnake Crotalus durissus spp has a wide geographic distribution in Brazil. Although responsible for only a low proportion of ophidian accidents, it is considered one of the most medically important species of venomous snakes due to the high mortality rate (1.87%). Snake venom is a complex phenotype commonly subjected to individual intraspecific, ontogenetic and geographic variability. Compositional differences in pooled venom used in the immunization process may impact the efficacy of the antivenom. In order to assure standardized high-quality antivenom, the potency of each Brazilian crotalic antivenom batch is determined against the 'Brazilian Crotalic Reference Venom' (BCRV). BCRV is produced by Instituto Butantan using venom obtained from the first milking of recently wild-caught C. d. terrificus specimens brought to the Institute. The decrease in the number of snake donations experienced in recent years can become a threat to the production of future batches of BCRV. To evaluate the feasibility of using venom from long-term captive animals in the formulation of BCRV, we have compared the proteomic, biochemical and biological profiles of C. d. terrificus venom pooled from captive specimens (CVP- captive venom pool) and BCRV. Electrophoretic and venomics analyses revealed a very similar venom composition profile, but also certain differences in toxins abundance, with some low abundant protein families found only in BCRV. Enzymatic (L-amino acid oxidase, phospholipase A2 and proteolytic) and biological (myotoxic and coagulant) activities showed higher values in CVP than in BCRV. CVP also possessed slightly higher lethal effect, although the Instituto Butantan crotalic antivenom showed equivalent potency neutralizing BCRV and CVP. Our results strongly suggest that venom from long-term captive C. d. terrificus might be a valid alternative to generate an immunization mixture of equivalent quality to the currently in use reference venom. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/32634448/Comparative_proteomic_profiling_and_functional_characterization_of_venom_pooled_from_captive_Crotalus_durissus_terrificus_specimens_and_the_Brazilian_crotalic_reference_venom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(20)30305-6 DB - PRIME DP - Unbound Medicine ER -