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YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora.
Int J Mol Sci. 2020 Jul 03; 21(13)IJ

Abstract

Despite the existence of considerable research on somatic embryogenesis (SE), the molecular mechanism that regulates the biosynthesis of auxins during the SE induction process remains unknown. Indole-3-acetic acid (IAA) is an auxin that is synthesized in plants through five pathways. The biosynthetic pathway most frequently used in this synthesis is the conversion of tryptophan to indol-3-pyruvic acid (IPA) by tryptophan aminotransferase of Arabidopsis (TAA) followed by the conversion of IPA to IAA by enzymes encoded by YUCCA (YUC) genes of the flavin monooxygenase family; however, it is unclear whether YUC-mediated IAA biosynthesis is involved in SE induction. In this study, we report that the increase of IAA observed during SE pre-treatment (plants in MS medium supplemented with 1-naphthaleneacetic acid (NAA) 0.54 µM and kinetin (Kin) 2.32 µM for 14 days) was due to its de novo biosynthesis. By qRT-PCR, we demonstrated that YUC gene expression was consistent with the free IAA signal found in the explants during the induction of SE. In addition, the use of yucasin to inhibit the activity of YUC enzymes reduced the signal of free IAA in the leaf explants and dramatically decreased the induction of SE. The exogenous addition of IAA restored the SE process in explants treated with yucasin. Our findings suggest that the biosynthesis and localization of IAA play an essential role during the induction process of SE in Coffea canephora.

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Authors+Show Affiliations

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 × 32 y 344 Col. Chuburná de Hidalgo, Mérida C.P. 97205, Mexico.

Pérez-Hernández C

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 × 32 y 344 Col. Chuburná de Hidalgo, Mérida C.P. 97205, Mexico.

Galaz-Ávalos RM

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 × 32 y 344 Col. Chuburná de Hidalgo, Mérida C.P. 97205, Mexico.

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 × 32 y 344 Col. Chuburná de Hidalgo, Mérida C.P. 97205, Mexico.

Aguilar-Hernández V

Catedrático CONACYT, Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida C.P. 97205, Mexico.

Loyola-Vargas VM

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 × 32 y 344 Col. Chuburná de Hidalgo, Mérida C.P. 97205, Mexico.

MeSH

Biosynthetic PathwaysCoffeaEnzyme InhibitorsGene Expression ProfilingGenes, PlantIndoleacetic AcidsMixed Function OxygenasesMultigene FamilyPlant Growth RegulatorsPlant ProteinsPlant Somatic Embryogenesis TechniquesTriazoles

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32635392

Citation

Uc-Chuc, Miguel A., et al. "YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required During the Somatic Embryogenic Induction Process in Coffea Canephora." International Journal of Molecular Sciences, vol. 21, no. 13, 2020.

Uc-Chuc MA, Pérez-Hernández C, Galaz-Ávalos RM, et al. YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora. Int J Mol Sci. 2020;21(13).

Uc-Chuc, M. A., Pérez-Hernández, C., Galaz-Ávalos, R. M., Brito-Argaez, L., Aguilar-Hernández, V., & Loyola-Vargas, V. M. (2020). YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora. International Journal of Molecular Sciences, 21(13). https://doi.org/10.3390/ijms21134751

Uc-Chuc MA, et al. YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required During the Somatic Embryogenic Induction Process in Coffea Canephora. Int J Mol Sci. 2020 Jul 3;21(13) PubMed PMID: 32635392.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora. AU - Uc-Chuc,Miguel A, AU - Pérez-Hernández,Cleyre, AU - Galaz-Ávalos,Rosa M, AU - Brito-Argaez,Ligia, AU - Aguilar-Hernández,Víctor, AU - Loyola-Vargas,Víctor M, Y1 - 2020/07/03/ PY - 2020/06/18/received PY - 2020/06/24/revised PY - 2020/06/28/accepted PY - 2020/7/9/entrez PY - 2020/7/9/pubmed PY - 2021/2/26/medline KW - Coffea canephora KW - YUCCA KW - auxin KW - localization KW - somatic embryogenesis KW - yucasin JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 13 N2 - Despite the existence of considerable research on somatic embryogenesis (SE), the molecular mechanism that regulates the biosynthesis of auxins during the SE induction process remains unknown. Indole-3-acetic acid (IAA) is an auxin that is synthesized in plants through five pathways. The biosynthetic pathway most frequently used in this synthesis is the conversion of tryptophan to indol-3-pyruvic acid (IPA) by tryptophan aminotransferase of Arabidopsis (TAA) followed by the conversion of IPA to IAA by enzymes encoded by YUCCA (YUC) genes of the flavin monooxygenase family; however, it is unclear whether YUC-mediated IAA biosynthesis is involved in SE induction. In this study, we report that the increase of IAA observed during SE pre-treatment (plants in MS medium supplemented with 1-naphthaleneacetic acid (NAA) 0.54 µM and kinetin (Kin) 2.32 µM for 14 days) was due to its de novo biosynthesis. By qRT-PCR, we demonstrated that YUC gene expression was consistent with the free IAA signal found in the explants during the induction of SE. In addition, the use of yucasin to inhibit the activity of YUC enzymes reduced the signal of free IAA in the leaf explants and dramatically decreased the induction of SE. The exogenous addition of IAA restored the SE process in explants treated with yucasin. Our findings suggest that the biosynthesis and localization of IAA play an essential role during the induction process of SE in Coffea canephora. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32635392/YUCCA_Mediated_Biosynthesis_of_the_Auxin_IAA_Is_Required_during_the_Somatic_Embryogenic_Induction_Process_in_Coffea_canephora_ DB - PRIME DP - Unbound Medicine ER -