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Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation.
Turk J Pharm Sci. 2020 Jun; 17(3):265-270.TJ

Abstract

Objectives

Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated.

Materials and Methods

After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h.

Results

Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content.

Conclusion

The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells.

Authors+Show Affiliations

Near East University Faculty of Pharmacy, Department of Biochemistry, Nicosia, North Cyprus.Near East University Faculty of Health Sciences, Department of Nutrition and Dietetics, Nicosia, North Cyprus.Manisa Celal Bayar University Faculty of Medicine, Department of Histology and Embryology, Manisa, Turkey.Near East University, Faculty of Medicine, Department of Pediatric Surgery, Nicosia, North Cyprus.Near East University Faculty of Pharmacy, Department of Pharmacognosy, Nicosia, North Cyprus.Manisa Celal Bayar University Faculty of Medicine, Department of Histology and Embryology, Manisa, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32636703

Citation

Becer, Eda, et al. "Obtaining Stem Cell Spheroids From Foreskin Tissue and the Effect of Corchorus Olitorius L. On Spheroid Proliferation." Turkish Journal of Pharmaceutical Sciences, vol. 17, no. 3, 2020, pp. 265-270.
Becer E, Soykut G, Kabadayi H, et al. Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation. Turk J Pharm Sci. 2020;17(3):265-270.
Becer, E., Soykut, G., Kabadayi, H., Mammadov, E., ÇaliŞ, İ., & Vatansever, S. (2020). Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation. Turkish Journal of Pharmaceutical Sciences, 17(3), 265-270. https://doi.org/10.4274/tjps.galenos.2019.05658
Becer E, et al. Obtaining Stem Cell Spheroids From Foreskin Tissue and the Effect of Corchorus Olitorius L. On Spheroid Proliferation. Turk J Pharm Sci. 2020;17(3):265-270. PubMed PMID: 32636703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of Corchorus olitorius L. on Spheroid Proliferation. AU - Becer,Eda, AU - Soykut,Günsu, AU - Kabadayi,Hilal, AU - Mammadov,Emil, AU - ÇaliŞ,İhsan, AU - Vatansever,Seda, Y1 - 2020/06/22/ PY - 2018/09/28/received PY - 2019/03/07/accepted PY - 2020/7/9/entrez PY - 2020/7/9/pubmed PY - 2020/7/9/medline KW - Corchorus olitorius KW - human foreskin KW - spheroid KW - stem cell SP - 265 EP - 270 JF - Turkish journal of pharmaceutical sciences JO - Turk J Pharm Sci VL - 17 IS - 3 N2 - Objectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells. SN - 2148-6247 UR - https://www.unboundmedicine.com/medline/citation/32636703/Obtaining_Stem_Cell_Spheroids_from_Foreskin_Tissue_and_the_Effect_of_Corchorus_olitorius_L._on_Spheroid_Proliferation L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32636703/ DB - PRIME DP - Unbound Medicine ER -
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