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Dynamic conjugate F-SHARP microscopy.
Light Sci Appl. 2020; 9:110.LS

Abstract

Optical microscopy is an indispensable tool in biomedical sciences, but its reach in deep tissues is limited due to aberrations and scattering. This problem can be overcome by wavefront-shaping techniques, albeit at limited fields of view (FOVs). Inspired by astronomical imaging, conjugate wavefront shaping can lead to an increased field of view in microscopy, but this correction is limited to a set depth and cannot be dynamically adapted. Here, we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing (F-SHARP). We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes. We demonstrate the power of the method by imaging with high resolution over extended FOV (>80 µm) deeper than 400 μm inside a mouse brain through a thinned skull.

Authors+Show Affiliations

Charité - Universitätsmedizin Berlin, Einstein Center for Neurosciences, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany.Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13092 Berlin, Germany.Institute for Biology, Humboldt University, Charitéplatz 1, 10117 Berlin, Germany.Institute for Biology, Humboldt University, Charitéplatz 1, 10117 Berlin, Germany.Institute for Biology, Humboldt University, Charitéplatz 1, 10117 Berlin, Germany.Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13092 Berlin, Germany.Charité - Universitätsmedizin Berlin, Einstein Center for Neurosciences, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32637077

Citation

Papadopoulos, Ioannis N., et al. "Dynamic Conjugate F-SHARP Microscopy." Light, Science & Applications, vol. 9, 2020, p. 110.
Papadopoulos IN, Jouhanneau JS, Takahashi N, et al. Dynamic conjugate F-SHARP microscopy. Light Sci Appl. 2020;9:110.
Papadopoulos, I. N., Jouhanneau, J. S., Takahashi, N., Kaplan, D., Larkum, M., Poulet, J., & Judkewitz, B. (2020). Dynamic conjugate F-SHARP microscopy. Light, Science & Applications, 9, 110. https://doi.org/10.1038/s41377-020-00348-x
Papadopoulos IN, et al. Dynamic Conjugate F-SHARP Microscopy. Light Sci Appl. 2020;9:110. PubMed PMID: 32637077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic conjugate F-SHARP microscopy. AU - Papadopoulos,Ioannis N, AU - Jouhanneau,Jean-Sebastien, AU - Takahashi,Naoya, AU - Kaplan,David, AU - Larkum,Matthew, AU - Poulet,James, AU - Judkewitz,Benjamin, Y1 - 2020/06/30/ PY - 2019/12/30/received PY - 2020/06/01/revised PY - 2020/06/15/accepted PY - 2020/7/9/entrez PY - 2020/7/9/pubmed PY - 2020/7/9/medline KW - Interference microscopy KW - Multiphoton microscopy SP - 110 EP - 110 JF - Light, science & applications JO - Light Sci Appl VL - 9 N2 - Optical microscopy is an indispensable tool in biomedical sciences, but its reach in deep tissues is limited due to aberrations and scattering. This problem can be overcome by wavefront-shaping techniques, albeit at limited fields of view (FOVs). Inspired by astronomical imaging, conjugate wavefront shaping can lead to an increased field of view in microscopy, but this correction is limited to a set depth and cannot be dynamically adapted. Here, we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing (F-SHARP). We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes. We demonstrate the power of the method by imaging with high resolution over extended FOV (>80 µm) deeper than 400 μm inside a mouse brain through a thinned skull. SN - 2047-7538 UR - https://www.unboundmedicine.com/medline/citation/32637077/Dynamic_conjugate_F-SHARP_microscopy DB - PRIME DP - Unbound Medicine ER -
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