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Description of Cryptosporidium ornithophilus n. sp. (Apicomplexa: Cryptosporidiidae) in farmed ostriches.
Parasit Vectors. 2020 Jul 08; 13(1):340.PV

Abstract

BACKGROUND

Avian cryptosporidiosis is a common parasitic disease that is caused by five species, which are well characterised at the molecular and biological level, and more than 18 genotypes for which we have limited information. In this study, we determined the occurrence and molecular characteristics of Cryptosporidium spp. in farmed ostriches in the Czech Republic.

METHODS

The occurrence and genetic identity of Cryptosporidium spp. were analysed by microscopy and PCR/sequencing of the small subunit rRNA, actin, HSP70 and gp60 genes. Cryptosporidium avian genotype II was examined from naturally and experimentally infected hosts and measured using differential interference contrast. The localisation of the life-cycle stages was studied by electron microscopy and histologically. Infectivity of Cryptosporidium avian genotype II for cockatiels (Nymphicus hollandicus (Kerr)), chickens (Gallus gallus f. domestica (L.)), geese (Anser anser f. domestica (L.)), SCID and BALB/c mice (Mus musculus L.) was verified.

RESULTS

A total of 204 individual faecal samples were examined for Cryptosporidium spp. using differential staining and PCR/sequencing. Phylogenetic analysis of small subunit rRNA, actin, HSP70 and gp60 gene sequences showed the presence of Cryptosporidium avian genotype II (n = 7) and C. ubiquitum Fayer, Santín & Macarisin, 2010 IXa (n = 5). Only ostriches infected with Cryptosporidium avian genotype II shed oocysts that were detectable by microscopy. Oocysts were purified from a pooled sample of four birds, characterised morphometrically and used in experimental infections to determine biological characteristics. Oocysts of Cryptosporidium avian genotype II measure on average 6.13 × 5.15 μm, and are indistinguishable by size from C. baileyi Current, Upton & Haynes, 1986 and C. avium Holubová, Sak, Horčičková, Hlásková, Květoňová, Menchaca, McEvoy & Kváč, 2016. Cryptosporidium avian genotype II was experimentally infectious for geese, chickens and cockatiels, with a prepatent period of four, seven and eight days post-infection, respectively. The infection intensity ranged from 1000 to 16,000 oocysts per gram. None of the naturally or experimentally infected birds developed clinical signs in the present study.

CONCLUSIONS

The molecular and biological characteristics of Cryptosporidium avian genotype II, described here, support the establishment of a new species, Cryptosporidium ornithophilus n. sp.

Authors+Show Affiliations

Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i, České Budějovice, Czech Republic. Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i, České Budějovice, Czech Republic. Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i, České Budějovice, Czech Republic.Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.Veterinary and Microbiological Sciences Department, North Dakota State University, Fargo, USA.Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i, České Budějovice, Czech Republic. kvac@paru.cas.cz. Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic. kvac@paru.cas.cz.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32641157

Citation

Holubová, Nikola, et al. "Description of Cryptosporidium Ornithophilus N. Sp. (Apicomplexa: Cryptosporidiidae) in Farmed Ostriches." Parasites & Vectors, vol. 13, no. 1, 2020, p. 340.
Holubová N, Tůmová L, Sak B, et al. Description of Cryptosporidium ornithophilus n. sp. (Apicomplexa: Cryptosporidiidae) in farmed ostriches. Parasit Vectors. 2020;13(1):340.
Holubová, N., Tůmová, L., Sak, B., Hejzlarová, A., Konečný, R., McEvoy, J., & Kváč, M. (2020). Description of Cryptosporidium ornithophilus n. sp. (Apicomplexa: Cryptosporidiidae) in farmed ostriches. Parasites & Vectors, 13(1), 340. https://doi.org/10.1186/s13071-020-04191-2
Holubová N, et al. Description of Cryptosporidium Ornithophilus N. Sp. (Apicomplexa: Cryptosporidiidae) in Farmed Ostriches. Parasit Vectors. 2020 Jul 8;13(1):340. PubMed PMID: 32641157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Description of Cryptosporidium ornithophilus n. sp. (Apicomplexa: Cryptosporidiidae) in farmed ostriches. AU - Holubová,Nikola, AU - Tůmová,Lenka, AU - Sak,Bohumil, AU - Hejzlarová,Adéla, AU - Konečný,Roman, AU - McEvoy,John, AU - Kváč,Martin, Y1 - 2020/07/08/ PY - 2020/02/24/received PY - 2020/06/15/accepted PY - 2020/7/10/entrez PY - 2020/7/10/pubmed PY - 2020/7/10/medline KW - C. ubiquitum KW - Cryptosporidium avian genotype II KW - Cryptosporidium ornithophilus n. sp. KW - Experimental infections KW - Occurrence KW - Oocyst size KW - PCR SP - 340 EP - 340 JF - Parasites & vectors JO - Parasit Vectors VL - 13 IS - 1 N2 - BACKGROUND: Avian cryptosporidiosis is a common parasitic disease that is caused by five species, which are well characterised at the molecular and biological level, and more than 18 genotypes for which we have limited information. In this study, we determined the occurrence and molecular characteristics of Cryptosporidium spp. in farmed ostriches in the Czech Republic. METHODS: The occurrence and genetic identity of Cryptosporidium spp. were analysed by microscopy and PCR/sequencing of the small subunit rRNA, actin, HSP70 and gp60 genes. Cryptosporidium avian genotype II was examined from naturally and experimentally infected hosts and measured using differential interference contrast. The localisation of the life-cycle stages was studied by electron microscopy and histologically. Infectivity of Cryptosporidium avian genotype II for cockatiels (Nymphicus hollandicus (Kerr)), chickens (Gallus gallus f. domestica (L.)), geese (Anser anser f. domestica (L.)), SCID and BALB/c mice (Mus musculus L.) was verified. RESULTS: A total of 204 individual faecal samples were examined for Cryptosporidium spp. using differential staining and PCR/sequencing. Phylogenetic analysis of small subunit rRNA, actin, HSP70 and gp60 gene sequences showed the presence of Cryptosporidium avian genotype II (n = 7) and C. ubiquitum Fayer, Santín & Macarisin, 2010 IXa (n = 5). Only ostriches infected with Cryptosporidium avian genotype II shed oocysts that were detectable by microscopy. Oocysts were purified from a pooled sample of four birds, characterised morphometrically and used in experimental infections to determine biological characteristics. Oocysts of Cryptosporidium avian genotype II measure on average 6.13 × 5.15 μm, and are indistinguishable by size from C. baileyi Current, Upton & Haynes, 1986 and C. avium Holubová, Sak, Horčičková, Hlásková, Květoňová, Menchaca, McEvoy & Kváč, 2016. Cryptosporidium avian genotype II was experimentally infectious for geese, chickens and cockatiels, with a prepatent period of four, seven and eight days post-infection, respectively. The infection intensity ranged from 1000 to 16,000 oocysts per gram. None of the naturally or experimentally infected birds developed clinical signs in the present study. CONCLUSIONS: The molecular and biological characteristics of Cryptosporidium avian genotype II, described here, support the establishment of a new species, Cryptosporidium ornithophilus n. sp. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/32641157/Description_of_Cryptosporidium_ornithophilus_n._sp._(Apicomplexa:_Cryptosporidiidae)_in_farmed_ostriches L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04191-2 DB - PRIME DP - Unbound Medicine ER -
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