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Tuning intermediate filament mechanics by variation of pH and ion charges.
Nanoscale. 2020 Jul 23; 12(28):15236-15245.N

Abstract

The cytoskeleton is formed by three types of filamentous proteins - microtubules, actin filaments, and intermediate filaments (IFs) - and enables cells to withstand external and internal forces. Vimentin is the most abundant IF protein in humans and assembles into 10 nm diameter filaments with remarkable mechanical properties, such as high extensibility and stability. It is, however, unclear to which extent these properties are influenced by the electrostatic environment. Here, we study the mechanical properties of single vimentin filaments by employing optical trapping combined with microfluidics. Force-strain curves, recorded at varying ion concentrations and pH values, reveal that the mechanical properties of single vimentin IFs are influenced by pH and ion concentration. By combination with Monte Carlo simulations, we relate these altered mechanics to electrostatic interactions of subunits within the filaments. We thus suggest possible mechanisms that allow cells to locally tune their stiffness without remodeling the entire cytoskeleton.

Authors+Show Affiliations

Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany. sarah.koester@phys.uni-goettingen.de.Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany. sarah.koester@phys.uni-goettingen.de.Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany. sarah.koester@phys.uni-goettingen.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32642745

Citation

Schepers, Anna V., et al. "Tuning Intermediate Filament Mechanics By Variation of pH and Ion Charges." Nanoscale, vol. 12, no. 28, 2020, pp. 15236-15245.
Schepers AV, Lorenz C, Köster S. Tuning intermediate filament mechanics by variation of pH and ion charges. Nanoscale. 2020;12(28):15236-15245.
Schepers, A. V., Lorenz, C., & Köster, S. (2020). Tuning intermediate filament mechanics by variation of pH and ion charges. Nanoscale, 12(28), 15236-15245. https://doi.org/10.1039/d0nr02778b
Schepers AV, Lorenz C, Köster S. Tuning Intermediate Filament Mechanics By Variation of pH and Ion Charges. Nanoscale. 2020 Jul 23;12(28):15236-15245. PubMed PMID: 32642745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tuning intermediate filament mechanics by variation of pH and ion charges. AU - Schepers,Anna V, AU - Lorenz,Charlotta, AU - Köster,Sarah, PY - 2020/7/10/pubmed PY - 2020/7/10/medline PY - 2020/7/10/entrez SP - 15236 EP - 15245 JF - Nanoscale JO - Nanoscale VL - 12 IS - 28 N2 - The cytoskeleton is formed by three types of filamentous proteins - microtubules, actin filaments, and intermediate filaments (IFs) - and enables cells to withstand external and internal forces. Vimentin is the most abundant IF protein in humans and assembles into 10 nm diameter filaments with remarkable mechanical properties, such as high extensibility and stability. It is, however, unclear to which extent these properties are influenced by the electrostatic environment. Here, we study the mechanical properties of single vimentin filaments by employing optical trapping combined with microfluidics. Force-strain curves, recorded at varying ion concentrations and pH values, reveal that the mechanical properties of single vimentin IFs are influenced by pH and ion concentration. By combination with Monte Carlo simulations, we relate these altered mechanics to electrostatic interactions of subunits within the filaments. We thus suggest possible mechanisms that allow cells to locally tune their stiffness without remodeling the entire cytoskeleton. SN - 2040-3372 UR - https://www.unboundmedicine.com/medline/citation/32642745/Tuning_intermediate_filament_mechanics_by_variation_of_pH_and_ion_charges L2 - https://doi.org/10.1039/d0nr02778b DB - PRIME DP - Unbound Medicine ER -
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