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[Effect of electroacupuncture on recognition memory and levels of Aβ, inflammatory factor proteins and aquaporin 4 in hippocampus of APP/PS1 double transgenic mice].
Zhen Ci Yan Jiu. 2020 Jun 25; 45(6):431-7.ZC

Abstract

OBJECTIVE

To investigate the effect of electroacupuncture (EA) at "Baihui "(GV20) and "Shenshu "(BL23) on activation of glial cells, expression of inflammatory factor proteins and aquaporin 4 (AQP4)in the hippocampus of amyloid precursor protein/presenilin-1 (APP/PS1) transgenic mice, so as to explore its mechanisms underlying improvement of Alzheimer's disease(AD).

METHODS

Twenty C57/BL6 background male APP695/PS1-dE9(APP/PS1) double transgenic mice (model group) and 20 wild type (WT) C57/BL6 mice (blank group) were respectively randomized into control and EA groups. EA (2 Hz/15 Hz, 1-2 mA) was applied to GV20 and bilateral BL23 for 30 min, once daily, 6 days a week for 4 weeks. The recognition memory ability was detected by novel object recognition tests in a behavior test box. The percentage of time spent in close interaction with novel object (C) relative to the total time was used to generate preference index. The contents of hippocampal β amyloid protein (Aβ)1-40 and Aβ1-42 were assayed using ELISA, and the expression levels of glial fibrillary acidic protein (GFAP), ionic calcium binding receptor molecule-1 (Iba-1), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proteins in the hippocampus measured by Western blot. The activities of hippocampal astrocytes (GFAP-labelled cells), microglia (Iba-1-labelled cells) and the polarity expression of AQP4 (for removing Aβ) were measured by immunohistochemistry.

RESULTS

The preference index was significantly decreased in the model group relatively to the blank control group (P<0.05) and considerably increased in the model+EA group relatively to the model group (P<0.05), suggesting an improvement of the recognition memory after EA. The contents of Aβ1-40 and Aβ1-42, immunoactivity of GFAP and Iba-1, expression levels of GFAP, Iba-1, IL-1β, IL-6 and TNF-α proteins were significantly higher in the model group than in the blank control group (P<0.01,P<0.05), while the AQP4 immunoactivity was notably lower in the model group than in the blank control group (P<0.05). Compared with the model group, the levels of Aβ1-40 and Aβ1-42, GFAP, Iba-1, IL-1β, IL-6 and TNF-α proteins, and the percentage of Aβ plaque area were significantly decreased in the model+EA group (P<0.01,P<0.05), and the immunoactivity of AQP4 was significantly increased in the mo-del+EA group (P<0.05). No significant changes were found in the above-mentioned indexes in the blank+EA group relevant to the blank control group (P>0.05)..

CONCLUSION

EA at GV20 and BL23 can reduce inflammatory reaction and Aβ level, suppress activation of astrocytes and microglia, and up-regulate expression of AQP4 in the hippocampus tissue in APP/PS1 transgenic mice, which may contribute to its effect in improving recognition memory ability, suggesting a role of EA intervention in delaying the development of AD via promoting the drainage of Aβ by the glymphatic system in the brain.

Authors+Show Affiliations

School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32643878

Citation

Wang, Lin-Mei, et al. "[Effect of Electroacupuncture On Recognition Memory and Levels of Aβ, Inflammatory Factor Proteins and Aquaporin 4 in Hippocampus of APP/PS1 Double Transgenic Mice]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 6, 2020, pp. 431-7.
Wang LM, Zhao TT, Zhou HP, et al. [Effect of electroacupuncture on recognition memory and levels of Aβ, inflammatory factor proteins and aquaporin 4 in hippocampus of APP/PS1 double transgenic mice]. Zhen Ci Yan Jiu. 2020;45(6):431-7.
Wang, L. M., Zhao, T. T., Zhou, H. P., Zhou, Z. Y., Huang, S., Ling, Y. L., & Shao, S. J. (2020). [Effect of electroacupuncture on recognition memory and levels of Aβ, inflammatory factor proteins and aquaporin 4 in hippocampus of APP/PS1 double transgenic mice]. Zhen Ci Yan Jiu = Acupuncture Research, 45(6), 431-7. https://doi.org/10.13702/j.1000-0607.190923
Wang LM, et al. [Effect of Electroacupuncture On Recognition Memory and Levels of Aβ, Inflammatory Factor Proteins and Aquaporin 4 in Hippocampus of APP/PS1 Double Transgenic Mice]. Zhen Ci Yan Jiu. 2020 Jun 25;45(6):431-7. PubMed PMID: 32643878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on recognition memory and levels of Aβ, inflammatory factor proteins and aquaporin 4 in hippocampus of APP/PS1 double transgenic mice]. AU - Wang,Lin-Mei, AU - Zhao,Tian-Tian, AU - Zhou,Han-Pu, AU - Zhou,Zi-Yi, AU - Huang,Shuo, AU - Ling,Yin-Lu, AU - Shao,Shui-Jin, PY - 2020/7/10/entrez PY - 2020/7/10/pubmed PY - 2020/9/2/medline KW - Alzheimer’s disease KW - Aquaporin 4 KW - Electroacupuncture KW - Glial cells KW - Inflammatory factors SP - 431 EP - 7 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 6 N2 - OBJECTIVE: To investigate the effect of electroacupuncture (EA) at "Baihui "(GV20) and "Shenshu "(BL23) on activation of glial cells, expression of inflammatory factor proteins and aquaporin 4 (AQP4)in the hippocampus of amyloid precursor protein/presenilin-1 (APP/PS1) transgenic mice, so as to explore its mechanisms underlying improvement of Alzheimer's disease(AD). METHODS: Twenty C57/BL6 background male APP695/PS1-dE9(APP/PS1) double transgenic mice (model group) and 20 wild type (WT) C57/BL6 mice (blank group) were respectively randomized into control and EA groups. EA (2 Hz/15 Hz, 1-2 mA) was applied to GV20 and bilateral BL23 for 30 min, once daily, 6 days a week for 4 weeks. The recognition memory ability was detected by novel object recognition tests in a behavior test box. The percentage of time spent in close interaction with novel object (C) relative to the total time was used to generate preference index. The contents of hippocampal β amyloid protein (Aβ)1-40 and Aβ1-42 were assayed using ELISA, and the expression levels of glial fibrillary acidic protein (GFAP), ionic calcium binding receptor molecule-1 (Iba-1), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proteins in the hippocampus measured by Western blot. The activities of hippocampal astrocytes (GFAP-labelled cells), microglia (Iba-1-labelled cells) and the polarity expression of AQP4 (for removing Aβ) were measured by immunohistochemistry. RESULTS: The preference index was significantly decreased in the model group relatively to the blank control group (P<0.05) and considerably increased in the model+EA group relatively to the model group (P<0.05), suggesting an improvement of the recognition memory after EA. The contents of Aβ1-40 and Aβ1-42, immunoactivity of GFAP and Iba-1, expression levels of GFAP, Iba-1, IL-1β, IL-6 and TNF-α proteins were significantly higher in the model group than in the blank control group (P<0.01,P<0.05), while the AQP4 immunoactivity was notably lower in the model group than in the blank control group (P<0.05). Compared with the model group, the levels of Aβ1-40 and Aβ1-42, GFAP, Iba-1, IL-1β, IL-6 and TNF-α proteins, and the percentage of Aβ plaque area were significantly decreased in the model+EA group (P<0.01,P<0.05), and the immunoactivity of AQP4 was significantly increased in the mo-del+EA group (P<0.05). No significant changes were found in the above-mentioned indexes in the blank+EA group relevant to the blank control group (P>0.05).. CONCLUSION: EA at GV20 and BL23 can reduce inflammatory reaction and Aβ level, suppress activation of astrocytes and microglia, and up-regulate expression of AQP4 in the hippocampus tissue in APP/PS1 transgenic mice, which may contribute to its effect in improving recognition memory ability, suggesting a role of EA intervention in delaying the development of AD via promoting the drainage of Aβ by the glymphatic system in the brain. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32643878/[Effect_of_electroacupuncture_on_recognition_memory_and_levels_of_Aβ_inflammatory_factor_proteins_and_aquaporin_4_in_hippocampus_of_APP/PS1_double_transgenic_mice]_ L2 - https://medlineplus.gov/alzheimersdisease.html DB - PRIME DP - Unbound Medicine ER -