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Disinfection of N95 masks artificially contaminated with SARS-CoV-2 and ESKAPE bacteria using hydrogen peroxide plasma: Impact on the reutilization of disposable devices.
Am J Infect Control. 2020 09; 48(9):1037-1041.AJ

Abstract

INTRODUCTION

One of the serious consequences of the SARS-CoV-2 pandemic is the shortage of protective equipment for health personnel. N95 masks are considered one of the essential protective equipment in the management of patients with COVID-19. The shortage of N95 masks implies potential health risks for health personnel and significant economic losses for the health institution. The objective of this work was to investigate the disinfection of N95 masks artificially contaminated with SARS-CoV-2 and ESKAPE bacteria by using hydrogen peroxide plasma.

MATERIAL AND METHODS

We examined the disinfection capacity of hydrogen peroxide plasma against the SARS-CoV-2 and 2 members of the ESKAPE bacteria (Acinetobacter baumannii and Staphylococcus aureus) through a study of artificial contamination in situ of N95 masks. Amplification of specific genes by real-time reverse transcription polymerase chain reaction of SARS-CoV-2 and microbiological culture of ESKAPE bacteria was performed before and after the disinfection process.

RESULTS

SARS-CoV-2 was not detected in all assays using 5 different concentrations of the virus, and A baumannii and S aureus were not cultivable with inoculums of 102 to 106 CFU after disinfection tests of N95 masks with hydrogen peroxide plasma.

CONCLUSION

Disinfection of N95 masks by using the hydrogen peroxide plasma technology can be an alternative for their reuse in a shortage situation. Implications for the use of disinfection technologies of N95 masks and the safety of health personnel are discussed.

Authors+Show Affiliations

División de Investigación, Hospital Juárez de México, Ciudad de México, Mexico.Laboratorio de Diagnóstico Molecular, Hospital Juárez de México, Ciudad de México, Mexico.Laboratorio de Diagnóstico Molecular, Hospital Juárez de México, Ciudad de México, Mexico.Servicio Central de Esterilización y Reprocesamiento, Hospital Juárez de México, Ciudad de México, Mexico.División de Investigación, Hospital Juárez de México, Ciudad de México, Mexico.División de Investigación, Hospital Juárez de México, Ciudad de México, Mexico.División de Investigación, Hospital Juárez de México, Ciudad de México, Mexico.Dirección de planeación estratégica, Hospital Juárez de México, Ciudad de México, Mexico.División Gineco Pediátrica, Hospital Juárez de México, Ciudad de México, Mexico.Laboratorio de Diagnóstico Molecular, Hospital Juárez de México, Ciudad de México, Mexico.Hospital General de Zona 2 Benigno Arriaga, Instituto Mexicano del Seguro Social (IMSS), Tequisquiapan, San Luis Potosí, México.Unidad de Vigilancia Epidemiologica Hospitalaria, Hospital Juárez de México, Ciudad de México, Mexico.División de Investigación, Hospital Juárez de México, Ciudad de México, Mexico. Electronic address: juanmanuelbello81@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32645473

Citation

Ibáñez-Cervantes, Gabriela, et al. "Disinfection of N95 Masks Artificially Contaminated With SARS-CoV-2 and ESKAPE Bacteria Using Hydrogen Peroxide Plasma: Impact On the Reutilization of Disposable Devices." American Journal of Infection Control, vol. 48, no. 9, 2020, pp. 1037-1041.
Ibáñez-Cervantes G, Bravata-Alcántara JC, Nájera-Cortés AS, et al. Disinfection of N95 masks artificially contaminated with SARS-CoV-2 and ESKAPE bacteria using hydrogen peroxide plasma: Impact on the reutilization of disposable devices. Am J Infect Control. 2020;48(9):1037-1041.
Ibáñez-Cervantes, G., Bravata-Alcántara, J. C., Nájera-Cortés, A. S., Meneses-Cruz, S., Delgado-Balbuena, L., Cruz-Cruz, C., Durán-Manuel, E. M., Cureño-Díaz, M. A., Gómez-Zamora, E., Chávez-Ocaña, S., Sosa-Hernández, O., Aguilar-Rojas, A., & Bello-López, J. M. (2020). Disinfection of N95 masks artificially contaminated with SARS-CoV-2 and ESKAPE bacteria using hydrogen peroxide plasma: Impact on the reutilization of disposable devices. American Journal of Infection Control, 48(9), 1037-1041. https://doi.org/10.1016/j.ajic.2020.06.216
Ibáñez-Cervantes G, et al. Disinfection of N95 Masks Artificially Contaminated With SARS-CoV-2 and ESKAPE Bacteria Using Hydrogen Peroxide Plasma: Impact On the Reutilization of Disposable Devices. Am J Infect Control. 2020;48(9):1037-1041. PubMed PMID: 32645473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disinfection of N95 masks artificially contaminated with SARS-CoV-2 and ESKAPE bacteria using hydrogen peroxide plasma: Impact on the reutilization of disposable devices. AU - Ibáñez-Cervantes,Gabriela, AU - Bravata-Alcántara,Juan Carlos, AU - Nájera-Cortés,Alan Steve, AU - Meneses-Cruz,Sergio, AU - Delgado-Balbuena,Laura, AU - Cruz-Cruz,Clemente, AU - Durán-Manuel,Emilio Mariano, AU - Cureño-Díaz,Monica Alethia, AU - Gómez-Zamora,Erika, AU - Chávez-Ocaña,Sonia, AU - Sosa-Hernández,Oscar, AU - Aguilar-Rojas,Antonio, AU - Bello-López,Juan Manuel, Y1 - 2020/07/06/ PY - 2020/05/11/received PY - 2020/06/22/revised PY - 2020/06/23/accepted PY - 2020/7/10/pubmed PY - 2020/9/12/medline PY - 2020/7/10/entrez KW - COVID-19 KW - Device reuse KW - Medical security KW - Protective equipment SP - 1037 EP - 1041 JF - American journal of infection control JO - Am J Infect Control VL - 48 IS - 9 N2 - INTRODUCTION: One of the serious consequences of the SARS-CoV-2 pandemic is the shortage of protective equipment for health personnel. N95 masks are considered one of the essential protective equipment in the management of patients with COVID-19. The shortage of N95 masks implies potential health risks for health personnel and significant economic losses for the health institution. The objective of this work was to investigate the disinfection of N95 masks artificially contaminated with SARS-CoV-2 and ESKAPE bacteria by using hydrogen peroxide plasma. MATERIAL AND METHODS: We examined the disinfection capacity of hydrogen peroxide plasma against the SARS-CoV-2 and 2 members of the ESKAPE bacteria (Acinetobacter baumannii and Staphylococcus aureus) through a study of artificial contamination in situ of N95 masks. Amplification of specific genes by real-time reverse transcription polymerase chain reaction of SARS-CoV-2 and microbiological culture of ESKAPE bacteria was performed before and after the disinfection process. RESULTS: SARS-CoV-2 was not detected in all assays using 5 different concentrations of the virus, and A baumannii and S aureus were not cultivable with inoculums of 102 to 106 CFU after disinfection tests of N95 masks with hydrogen peroxide plasma. CONCLUSION: Disinfection of N95 masks by using the hydrogen peroxide plasma technology can be an alternative for their reuse in a shortage situation. Implications for the use of disinfection technologies of N95 masks and the safety of health personnel are discussed. SN - 1527-3296 UR - https://www.unboundmedicine.com/medline/citation/32645473/Disinfection_of_N95_masks_artificially_contaminated_with_SARS_CoV_2_and_ESKAPE_bacteria_using_hydrogen_peroxide_plasma:_Impact_on_the_reutilization_of_disposable_devices_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-6553(20)30639-8 DB - PRIME DP - Unbound Medicine ER -